Published Papers
View all published papers related to VISQUE® and LUCEON.
Title
Year
System
Regulation of cerebral blood flow boosts precise brain targeting of vinpocetine-derived ionizable-lipidoid nanoparticles
2024
in vivo System
- Title
- Regulation of cerebral blood flow boosts precise brain targeting of vinpocetine-derived ionizable-lipidoid nanoparticles
- Description
- In this study, researchers demonstrated that vinpocetine-derived ionizable-lipidoid nanoparticles (VIP) effectively enhance brain targeting. In the brain-targeting experiment, LNP@DiD was administered to healthy mice via the tail vein. Fluorescence images were captured at specified time intervals (0.5 h, 1 h, 2 h, 4 h, and 8 h) using the VISQUE in vivo Smart-LF System. The nanoparticles containing VIP exhibited significantly higher fluorescence intensity in the brain compared to those without VIP, confirming their robust brain-targeting capability. Additionally, major organs were collected from the mice at each time point for ex vivo imaging. Mice treated with VIP showed stronger fluorescence signals in the brain compared to the positive control, GSH-LNP, which utilized glutathione (GSH) as the targeting agent.
- Journal
- Nat Commun. 2024; 15: 3987.
BMP7-Loaded Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorate Liver Fibrosis by Targeting Activated Hepatic Stellate Cells
2024
in vivo System
- Title
- BMP7-Loaded Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorate Liver Fibrosis by Targeting Activated Hepatic Stellate Cells
- Description
- "The study focuses on enhancing the healing properties of small extracellular vesicles derived from human umbilical cord mesenchymal stem cells (hucMSC-sEVs). Due to their tendency to non-specific distribution in vivo and be partially removed by monocular macrophages, natural hucMSC-sEVs are not fully effective when administered systemically. To address this, researchers have modified these vesicles at the genetic level to overexpress the anti-fibrotic gene bone morphogenic protein7(BMP7) in partner cell.
In study, two mice from each group received injections of different types of small extracellular vesicles (sEVs) into their tail veins. The researchers tracked the sEVs’ movement through the body using the VISQUE in vivo system at various intervals up to 72 hours after injection. The imaging revealed that the BMP7-loaded sEVs were effectively directed to the liver of the mice. These results demonstrated hucMSC-sEVs primarily accumulated in the liver and had a moderate ability to target activated hepatic stellate cells (aHSCs), which was further improved by incorporating BMP7 into the sEVs." - Journal
- Int J Nanomedicine. 2024; 19: 3475–3495.
Role of gut-derived bacterial lipopolysaccharide and peripheral TLR4 in immobilization stress-induced itch aggravation in a mouse model of atopic dermatitis
2024
in vivo System
- Title
- Role of gut-derived bacterial lipopolysaccharide and peripheral TLR4 in immobilization stress-induced itch aggravation in a mouse model of atopic dermatitis
- Description
- This study elucidates the mechanisms by which emotional stress exacerbates itch sensation and inflammation in the skin of patients with atopic dermatitis (AD). The researchers investigate the role of gut-derived bacterial components, specifically lipopolysaccharides (LPS), in this process, focusing on how chronic stress impacts intestinal barrier function and its subsequent effects on skin inflammation and itch in a mouse model of AD. VISQUE InVivo Smart LF was employed to visualize the whole-body distribution of Cy5.5-labeled bacterial LPS. On the final day of the daily immobilization stress (IMO) period, nude mice were anesthetized, and their rectums were isolated. Cy5.5-labeled LPS was injected into the proximal colon, and the abdominal wall was sutured. The imaging showed how bacterial LPS permeates and distributes in the body under chronic stress conditions, providing insights into the role of gut-derived LPS in exacerbating skin inflammation and itch in the context of chronic stress and AD.
- Journal
- Sci Rep. 2024; 14: 6263.
Modulation of chronic obstructive pulmonary disease progression by antioxidant metabolites from Pediococcus pentosaceus: enhancing gut probiotics abundance and the tryptophan-melatonin pathway
2024
in vivo System
- Title
- Modulation of chronic obstructive pulmonary disease progression by antioxidant metabolites from Pediococcus pentosaceus: enhancing gut probiotics abundance and the tryptophan-melatonin pathway
- Description
- "This study focused on evaluating the therapeutic potential of probiotics for Chronic obstructive pulmonary disease (COPD), a condition closely associated with oxidative stress. They tested the probiotic Pediococcus pentosaceus SMM914 could alleviate COPD symptoms and slow disease progression.
The study used VISQUE InVivo Smart and CleVue software for NIR fluorescence imaging to track the colonization of the probiotic SMM914 in the body. Using Cy5.5-d-lys labeled SMM914 mouse measured at 15 min, 45 min, and 4 hours after oral administration. The results showed that 45 minutes post-administration, most of the bacteria were located in the jejunum. After 2.5 hours, the bacteria concentrated in the ileum and colon, and by 4 hours, the fluorescence was primarily in the ileum, suggesting that the bacteria were nearing excretion. Additionally, the supplementation with SMM914 not only increased serotonin production but also improved the activity of the DDC enzyme in an inflammatory setting, supporting the probiotic's potential therapeutic effects in managing COPD." - Journal
- Gut Microbes. 2024; 16(1): 2320283.
Promoting collateral formation in type 2 diabetes mellitus using ultra-small nanodots with autophagy activation and ROS scavenging
2024
in vivo System
- Title
- Promoting collateral formation in type 2 diabetes mellitus using ultra-small nanodots with autophagy activation and ROS scavenging
- Description
- The study investigates the potential of molybdenum disulfide nanodots (MoS2 NDs) in promoting collateral formation and improving prognosis in type 2 diabetes mellitus (T2DM) by enhancing endothelial autophagy and scavenging reactive oxygen species (ROS). The Vieworks in vivo flourescence imaging system was used to assess the biodistribution of Cy5.5-labeled MoS2 NDs in ischemic muscles of a hindlimb ischemia mouse model to observe how these nanodots localize in the body post-injection. The imaging showed successful biodistribution of MoS2 NDs to the ischemic sites, indicating their effective delivery to targeted areas in the body
- Journal
- J Nanobiotechnology. 2024; 22: 85.
Pharmaceutical Manipulation of Mitochondrial F0F1‐ATP Synthase Enables Imaging and Protection of Myocardial Ischemia/Reperfusion Injury Through Stress‐induced Selective Enrichment
2023
in vivo System
- Title
- Pharmaceutical Manipulation of Mitochondrial F0F1‐ATP Synthase Enables Imaging and Protection of Myocardial Ischemia/Reperfusion Injury Through Stress‐induced Selective Enrichment
- Description
- This study aims to assess the ability of IR-780, a near-infrared dye, to image and protect myocardial tissue from ischemia/reperfusion (I/R) injury by selectively targeting the injured cardiomyocytes. VISQUE In Vivo Smart-LF was employed to visualize IR-780’s accumulation in myocardial tissue in a pig I/R model, aiding in the real-time imaging of the injury site. The imaging demonstrated selective retention of IR-780 in ischemic areas, allowing for effective visualization of at-risk tissue and showing a reduction in I/R-induced cell death, which suggests potential cardioprotective effects.
- Journal
- Adv Sci (Weinh). 2023 Dec 14;11(9):2307880.
Imaging assessment of photosensitizer emission induced by radionuclide-derived Cherenkov radiation using charge-coupled device optical imaging and long-pass filters
2023
in vivo System
- Title
- Imaging assessment of photosensitizer emission induced by radionuclide-derived Cherenkov radiation using charge-coupled device optical imaging and long-pass filters
- Description
- This study seeks to find a cost-effective method to confirm the excitation of photosensitizers (PS) by radionuclide-derived Cherenkov radiation (CR) and distinguish the resulting fluorescence emission. Tetrakis (4-carboxyphenyl) porphyrin (TCPP) was selected as a model PS, and the beta-emitting isotope Copper-64 was used as a CR-producing radionuclide. VISQUE InVivo Smart was used to confirm the inherent fluorescence characteristics of TCPP emission with filters for blue light (390-490 nm) excitation and red light (690-740 nm) emission. Combining other experiments, the researchers conclude that a simple method utilizing a CCD optical imaging system with different high-transmission long-pass filters and subtraction image processing can effectively assess if photosensitizer candidates are excited by radionuclide-derived Cherenkov radiation to generate fluorescence emission.
- Journal
- World J Radiol. 2023 Nov 28; 15(11): 315–323.
Development of a fibroblast activation protein-targeted PET/NIR dual-modality probe and its application in head and neck cancer
2023
in vivo System
- Title
- Development of a fibroblast activation protein-targeted PET/NIR dual-modality probe and its application in head and neck cancer
- Description
- This study developed the PET/NIR probe [68Ga]Ga-FAP-2286-ICG, targeting fibroblast activation protein (FAP) found in cancer-associated fibroblasts in solid tumors like head and neck squamous cell carcinoma (HNSCC). Fluorescence imaging using the VISQUE InVivo Smart-LF confirmed the probe's effectiveness in guiding surgical resections for precise tumor removal. U87MG tumor-bearing mice were imaged post-injection to identify the optimal timing for fluorescence imaging (FI), showing significant contrast as early as 0.5 hours, peaking at 24 hours and remaining elevated until 72 hours, indicating the ideal window for surgery. Unlike free ICG, which showed no tumor fluorescence, FAP-2286-ICG demonstrated enhanced targeting and retention. The imaging also revealed significant fluorescence in the liver and kidneys, indicating metabolic processing, and residual fluorescence post-surgery highlighted its potential for detecting remaining cancerous tissues.
- Journal
- Front Bioeng Biotechnol. 2023 Nov 3;11:1291824.
Rapid and Widespread Distribution of Intranasal Small Extracellular Vesicles Derived from Mesenchymal Stem Cells throughout the Brain Potentially via the Perivascular Pathway
2023
in vivo System
- Title
- Rapid and Widespread Distribution of Intranasal Small Extracellular Vesicles Derived from Mesenchymal Stem Cells throughout the Brain Potentially via the Perivascular Pathway
- Description
- The study aims to explore the distribution and potential therapeutic benefits of small extracellular vesicles (sEVs) derived from mesenchymal stem cells (MSCs) when administered intranasally, emphasizing their rapid and extensive delivery through the perivascular pathway. A VISQUE In Vivo Smart-LF system was utilized to image the distribution of Cy7-labeled MSC-sEVs in mice at 0.5, 2, 6, and 24 hours post-intranasal administration. Additionally, ex vivo imaging was performed to compare delivery to different organs. This data confirmed the fast and centralized distribution of intranasal MSC-sEVs.
- Journal
- Pharmaceutics. 2023 Nov; 15(11): 2578.
Exosomal mRNAs for Angiogenic–Osteogenic Coupled Bone Repair
2023
in vivo System
- Title
- Exosomal mRNAs for Angiogenic–Osteogenic Coupled Bone Repair
- Description
- The main goal of the study is to develop a scalable and efficient method for producing therapeutic small extracellular vesicles (sEVs) loaded with mRNAs for bone tissue regeneration. The Vieworks VISQUE in vivo Smarr-LF was employed to perform fluorescence imaging of PKH26-labeled sEVs within the PEGS-A hydrogel and standard scaffolds in a rat model. The imaging showed strong PKH26 signals, indicating successful delivery and distribution of the sEVs. Notably, the PEGS-A hydrogel demonstrated significantly higher fluorescence intensity compared to the standard scaffold after three days. This imaging confirmed that the sEVs were retained and released more effectively within the hydrogel, leading to sustained therapeutic presence at the defect site over a two-week period.
- Journal
- Adv Sci (Weinh). 2023 Oct 17;10(33):2302622.
Sustained delivery of triamcinolone acetonide from a thermosensitive microemulsion gel system for the treatment of sensorineural hearing loss
2023
in vivo System
- Title
- Sustained delivery of triamcinolone acetonide from a thermosensitive microemulsion gel system for the treatment of sensorineural hearing loss
- Description
- The study aims to develop and evaluate a thermosensitive microemulsion gel (MEG) system for the sustained delivery of triamcinolone acetonide (TA) to treat sensorineural hearing loss (SNHL) by improving drug retention and therapeutic efficacy in the inner ear. The VISQUE InVivo Smart-LF system was used to monitor the in vivo distribution and retention of Cy7-labeled TA-MEG in the inner ear of mice after intratympanic administration. This imaging system helped track the gel's localization and persistence over time. Imaging results demonstrated that the TA-MEG formulation exhibited sustained retention in the cochlea, with significant localization observed up to 6 days post-administration. This prolonged presence indicated effective gelation and slow degradation, validating the MEG system's potential for sustained drug delivery in SNHL treatment.
- Journal
- Drug Deliv. 2023; 30(1): 2242003.
Site-selective superassembly of biomimetic nanorobots enabling deep penetration into tumor with stiff stroma
2023
in vivo System
- Title
- Site-selective superassembly of biomimetic nanorobots enabling deep penetration into tumor with stiff stroma
- Description
- The primary objective of the study is to develop and evaluate a new class of nanorobots designed to improve tumor penetration and drug delivery for treating triple-negative breast cancer (TNBC). The VISQUE InVivo Elite was used to monitor tumor growth and evaluate the distribution of UHHTN(Urchin Head/Hollow Tail Nanostructures)/DOX(Doxorubicin) nanorobots in mouse models. Researchers conducted bioluminescence imaging to monitor tumor progression and performed ex vivo imaging of various organs at different time intervals after the nanorobot injection. This imaging approach provided detailed insights into the accumulation of the nanorobots in the tumor compared to other tissues, confirming their targeted delivery and enhancing understanding of their therapeutic efficacy. The results showed that the UHHTN/DOX nanorobots, combined with photothermal therapy, significantly reduced tumor growth and improved survival rates in treated mice, demonstrating their potential for effective cancer treatment.
- Journal
- Nat Commun. 2023 Aug 2;14:4628.
Activation of autophagy by in situ Zn2+ chelation reaction for enhanced tumor chemoimmunotherapy
2023
in vivo System
- Title
- Activation of autophagy by in situ Zn2+ chelation reaction for enhanced tumor chemoimmunotherapy
- Description
- The main goal of this study is to develop a nanoplatform that activates autophagy through in situ zinc ion (Zn²⁺) chelation, aiming to enhance the efficacy of tumor chemoimmunotherapy. VISQUE InVivo Elite was used to monitor the biodistribution and metabolism of Cy5.5-labeled DSF@Zn-DMSNs in tumor-bearing mice at various time points post-administration. Imaging results showed that the DSF@Zn-DMSNs had widespread and prolonged retention in tumor tissues. This retention confirmed the effective targeting and accumulation of the nanoparticles within the tumor, which correlated with the enhanced therapeutic outcomes observed in the study, including significant tumor growth inhibition and improved immune responses.
- Journal
- Bioact Mater. 2023 Nov; 29: 116–131.
Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics
2023
in vivo System
- Title
- Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics
- Description
- The study aimed to investigate the herapeutic potential of tumor-resident bacteria and their association with photosynthetic bacteria for targeted cancer immunotherapy. VISQUE InVivo Smart-LF was employed to monitor the near-infrared fluorescence (NIR FL) emitted by the bacteria in tumor-bearing mice, providing insights into their targeting efficacy and biodistribution. Mice received intravenous injections of bacterial suspensions, and imaging was performed five days post-injection to visualize the NIR FL intensity across the entire body, specifically focusing on tumor sites. The results indicated a strong tumor-targeting effect, with significant fluorescence observed in targeted tumors compared to other tissues, highlighting the effectiveness of the bacterial treatments in suppressing tumor growth and inducing durable antitumor immunity. Overall, the imaging demonstrated that these bacteria could serve as a promising therapeutic strategy for cancer treatment.
- Journal
- Adv Sci (Weinh). 2023 May 7;10(20):2301679.
Synergistic Encapsulation of Paclitaxel and Sorafenib by Methoxy Poly(Ethylene Glycol)-b-Poly(Caprolactone) Polymeric Micelles for Ovarian Cancer Therapy
2023
in vivo System
- Title
- Synergistic Encapsulation of Paclitaxel and Sorafenib by Methoxy Poly(Ethylene Glycol)-b-Poly(Caprolactone) Polymeric Micelles for Ovarian Cancer Therapy
- Description
- The main goal of this study is to develop and evaluate methoxy poly(ethylene glycol)-b-poly(caprolactone) (mPEG-b-PCL) polymeric micelles for the co-delivery of paclitaxel (PTX) and sorafenib (SRF) to enhance the treatment efficacy against ovarian cancer (OC) by exploiting the synergistic effects of these two drugs. Smart-LF was used in this study to monitor the in vivo bioluminescent imaging (BLI) of tumors in xenograft nude mice models. This system helped evaluate the tumor growth inhibition (TGI) percentage before and after drug administration. The imaging results using Smart-LF showed that the PTX/SRF micelle formulation exhibited significant tumor growth inhibition compared to the control and other treatment groups. The bioluminescence signal intensity measurements indicated effective suppression of tumor growth in the experimental groups treated with the micellar formulation.
- Journal
- Pharmaceutics. 2023 Apr; 15(4): 1206.
Efficacy of recombinant Bacillus Calmette-Guérin containing dltA in in vivo three-dimensional bio-printed bladder cancer-on-a-chip and ex vivo orthotopic mouse model
2023
in vivo System
- Title
- Efficacy of recombinant Bacillus Calmette-Guérin containing dltA in in vivo three-dimensional bio-printed bladder cancer-on-a-chip and ex vivo orthotopic mouse model
- Description
- The main goal of the paper is to evaluate the efficacy and optimal dosage of recombinant Bacillus Calmette-Guérin containing dltA (rBCG-dltA) in treating bladder cancer, using both a three-dimensional bio-printed bladder cancer-on-a-chip model and an orthotopic bladder cancer mouse model. The Vieworks VISQUE InVivio Smart-LF was used to monitor bioluminescence in the mouse bladder, enabling researchers to observe tumor progression and regression after treatment with rBCG-dltA. Smart-LF demonstrated that the rBCG-dltA treatment, particularly at higher dosages, significantly reduced tumor intensity compared to controls, indicating its potential antitumor efficacy(icu-64-296).
- Journal
- Investig Clin Urol. 2023 Mar 29;64(3):296–305
Wavelength engineerable porous organic polymer photosensitizers with protonation triggered ROS generation
2023
in vivo System
- Title
- Wavelength engineerable porous organic polymer photosensitizers with protonation triggered ROS generation
- Description
- The primary objective of this research is to develop a wavelength-engineerable, protonation-triggered porous organic polymer (POP) that can efficiently generate reactive oxygen species (ROS) for applications in biomedicine and photocatalysis. The focus is on creating a novel imidazolium-based POP that facilitates ROS generation without relying on metal ions or heavy atoms, addressing the challenge of maintaining high ROS yield while allowing for tunable excitation wavelengths. VISQUE InVivo Elite was used for the fluorescence imaging of U87MG-xenograft mice. The mice were imaged 24 hours after they were administered PBS, KUP-1, and O2@KUP-1 to assess the biodistribution and effectiveness of the developed photosensitizer. The imaging data indicated that O2@KUP-1 showed enhanced fluorescence signals compared to KUP-1 and PBS, demonstrating the effectiveness of the POP in generating ROS in vivo. This result underscores the potential of the developed polymer for applications in targeted cancer therapies and other biomedical fields.
- Journal
- Nat Commun. 2023; 14: 1498.
Novel NF-κB reporter mouse for the non-invasive monitoring of inflammatory diseases
2023
in vivo System
- Title
- Novel NF-κB reporter mouse for the non-invasive monitoring of inflammatory diseases
- Description
- "The main goal of the study was to create and evaluate a novel NF-kB luciferase reporter mouse model that enables non-invasive, real-time tracking of inflammatory responses in whole-body and cell-specific contexts.
The Vieworks VISQUE InVivo ART100 imaging system was used to capture bioluminescence signals in the mice, allowing researchers to visualize inflammation progression in different models, such as those induced by lipopolysaccharide (LPS) or diet. The result showed increased bioluminescence in targeted tissues correlating with inflammatory activity, validating the reporter mice’s capacity to reflect inflammation accurately and dynamically." - Journal
- Sci Rep. 2023 Mar 2;13:3556.
Bone tumor-homing nanotherapeutics for prolonged retention in tumor microenvironment and facilitated apoptotic process via mevalonate pathway inhibition
2023
in vivo System
- Title
- Bone tumor-homing nanotherapeutics for prolonged retention in tumor microenvironment and facilitated apoptotic process via mevalonate pathway inhibition
- Description
- This paper aims to create and assess bone tumor-targeting polymeric nanotherapeutics (NTs) designed to enhance drug retention within the tumor microenvironment (TME) and improve the effectiveness of bone cancer treatments. The research involves the use of alendronate-decorated chondroitin sulfate A-graft-poly(lactide-co-glycolide) (PLCSA-AD) combined with the chemotherapy drug doxorubicin (DOX) to inhibit the mevalonate pathway, a key metabolic process in tumor cells, thereby inducing apoptosis (cell death) and enhancing therapeutic outcomes. VISQUE InVivo Smart was employed to visualize and verify the accumulation of PLCSA-AD nanotherapeutics in bone tumor tissue in a mouse xenograft model. Cy5.5-labeled NTs were injected into the tail vein of tumor-bearing mice, with imaging performed at 0, 1, 3, 5, 9, and 24 hours post-injection. Subsequently, the tumors and major organs were dissected for ex vivo analysis. The imaging results revealed a significant increase in tumor accumulation of the PLCSA-AD nanotherapeutics, showing a 2.64-fold higher radiant efficiency at 24 hours and a 1.73-fold higher tumor disposition ex vivo compared to the non-alendronate-decorated variant (PLCSA).
- Journal
- Mater Today Bio. 2023 Apr; 19: 100591.
Endocrine modulation of brain-skeleton axis driven by neural stem cell-derived perilipin 5 in the lipid metabolism homeostasis for bone regeneration
2023
in vivo System
- Title
- Endocrine modulation of brain-skeleton axis driven by neural stem cell-derived perilipin 5 in the lipid metabolism homeostasis for bone regeneration
- Description
- "The primary aim of the paper is to explore how neural stem cell-derived exosomes, particularly those containing perilipin 5 (PLIN5), contribute to lipid metabolism regulation to enhance bone regeneration via an endocrine axis linking the brain and skeleton.
The Vieworks VISQUE InVivo Elite imaging system was used to track fluorescently labeled neural stem cell-derived exosomes as they traveled from the brain to bone injury sites, allowing researchers to visualize their distribution and homing capabilities. Results confirmed that the exosomes reached the bone injury areas, highlighting their potential to aid bone regeneration by modulating lipid metabolism in metabolic disorder conditions." - Journal
- Mol Ther. 2023 Feb 9;31(5):1293–1312.
Overcoming the On‐Target Toxicity in Antibody‐Mediated Therapies via an Indirect Active Targeting Strategy
2023
in vivo System
- Title
- Overcoming the On‐Target Toxicity in Antibody‐Mediated Therapies via an Indirect Active Targeting Strategy
- Description
- The primary objective of this study is to address and mitigate the on-target toxicity that is often associated with antibody-mediated therapy, specifically targeting tumor-associated antigens. The researchers aim to develop a method to reduce the adverse effects of antibody treatment by using a strategy that involves masking the antibody's binding domain with a cleavable peptide, which is then selectively removed in the tumor microenvironment. This approach seeks to enhance the specificity and safety of antibody-mediated cancer therapies. VISQUE in vivo Smart-LF was used for the ex vivo images of the fluorescently labeled antibodies. This allowed for temporal observation of how the antibodies circulated and accumulated in the tumors versus normal tissues, providing critical data on the effectiveness of the masking strategy. The images demonstrated that the engineered antibodies, with the masking peptide, showed reduced accumulation in non-tumor tissues compared to the unmodified antibodies. This selective localization was crucial to confirm that the peptide masking approach effectively reduced on-target toxicity without compromising the antibody's ability to target tumors.
- Journal
- Adv Sci (Weinh). 2023 Mar; 10(9): 2206912.
Repurpose dasatinib and quercetin: Targeting senescent cells ameliorates postmenopausal osteoporosis and rejuvenates bone regeneration
2023
in vivo System
- Title
- Repurpose dasatinib and quercetin: Targeting senescent cells ameliorates postmenopausal osteoporosis and rejuvenates bone regeneration
- Description
- "This study demonstrated that the combination of dadatinib and quercetin (DQ) can effectively improve postmenopausal osteoporosis (PMO) and enhance born health. Osteoporosis is one of the prevalnet chronic disease in the elderly, especially postmenopausal women. Current treatments for PMO often have shown adverse effects. DQ has shown potential in treating various age-related conditions. However, its effectiveness in PMO was not investigated until now.
VISQUE InVivo ART100 used to capture quantify fluorescence intensity of the released substance in vivo. The device was used to take images of the rat femoral defect area at different time points (1st, 3rd, and 5th day) to monitor the release of the substances. The fluorescence intensity of the released quercetin (using calcein as a model) and BMP2 (modified with sulfo-cyanine7) was measured. This study confirms that DQ can ameliorate PMO." - Journal
- Bioact Mater. 2023 Jul; 25: 13–28.
Nanoformulation of the K-Ras(G12D)-inhibitory peptide KS-58 suppresses colorectal and pancreatic cancer-derived tumors
2023
in vivo System
- Title
- Nanoformulation of the K-Ras(G12D)-inhibitory peptide KS-58 suppresses colorectal and pancreatic cancer-derived tumors
- Description
- The main goal of the paper is to develop and evaluate a nanoformulation of the K-Ras(G12D)-inhibitory peptide KS-58 for its effectiveness in suppressing colorectal and pancreatic cancer-derived tumors. The researchers aimed to improve the delivery and efficacy of the KS-58 peptide, which targets the K-Ras(G12D) mutation, a common and challenging mutation in these types of cancers. Vieworks VISQUE InVivo Smart-LF was used to monitor and evaluate the tumor growth and the therapeutic effect of the KS-58 nanoformulation in live animal models. The imaging with VISQUE InVivo Smart-LF provided clear bioluminescent images that allowed the researchers to assess the effectiveness of the KS-58 nanoformulation. The results showed significant suppression of tumor growth in the treated groups compared to the control groups. The data obtained from the imaging system supported the study's goal by demonstrating that the nanoformulated KS-58 peptide effectively inhibits tumor growth in colorectal and pancreatic cancer models, thus validating the therapeutic potential of this approach.
- Journal
- Sci Rep. 2023; 13: 518.
Potential Alzheimer's disease therapeutic nano-platform: Discovery of amyloid-beta plaque disaggregating agent and brain-targeted delivery system using porous silicon nanoparticles
2023
in vivo System
- Title
- Potential Alzheimer's disease therapeutic nano-platform: Discovery of amyloid-beta plaque disaggregating agent and brain-targeted delivery system using porous silicon nanoparticles
- Description
- "This study introduces a new agent for breaking down amyloid-beta(Aβ) plaque and a targeted delivery system for Alzheimer's disease(AD) treatment usinf porous silicon nanoparticles(pSiNPs). The nano-formulation, named BiotiCaCl2-ANA-pSiNPs (BCAP), effectively delivered a significant amount of the therapeutic agent 6-amino-2-naphthalenesulfonic acid (ANA) to the AD brain. This led to the disaggregation of amyloid plaques and improved memory in an AD mouse model.
Fluorescence imaging with VISQUE InVivo Elit was used to monitor the distribution of BCAP in the body. The results indicated that BCAP accumulated significantly in the AD brain, demonstrating its superior brain-targeting capability. Imaging also confirmed that BCAP effectively disaggregated Aβ plaques in vivo, reducing their presence in the AD brain. The study demonstrated that BCAP disaggregated Aβ plaques with high efficacy in vitro and showed high biocompatibility in vivo, leading to improved memory and reduced inflammation." - Journal
- Bioact Mater. 2023 Jun; 24: 497–506.
DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma
2023
in vivo System
- Title
- DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma
- Description
- The study investigates the role of diacylglycerol kinase B (DGKB) in the radioresistance of glioblastoma (GBM) cells. The researchers seek to understand how the downregulation of DGKB, which regulates the intracellular concentration of diacylglycerol (DAG), contributes to the resistance of GBM cells to radiotherapy. By exploring the mechanisms of DGKB downregulation and its effects on lipid metabolism, the study aims to identify potential therapeutic targets to overcome GBM radioresistance. VISQUE in vivo SmartLF was employed for bioluminescent imaging to track the tumor size and progression after irradiation in mice implanted with luciferase-expressing GBM cells. This imaging visualized the effects of radiotherapy combined with DGKB overexpression on tumor growth. It was revealed that radiation alone had minimal effects on tumor growth. However, when combined with DGKB overexpression, there was a significant reduction in tumor growth by approximately 62.19%.
- Journal
- Cell Rep Med. 2023 Jan 17; 4(1): 100880.
Development of an alginate-gelatin bioink enhancing osteogenic differentiation by gelatin release
2023
in vivo System
- Title
- Development of an alginate-gelatin bioink enhancing osteogenic differentiation by gelatin release
- Description
- This study aimed to enhance the osteogenic activity in a hydrogel by integrating gelatin, which supports both encapsulated and nearby cells. Methacrylate-modified alginate (MA-alginate) was selected for its low cell adhesion properties. VISQUE inVivo Smart system was used to monitor gelatin levels in hydrogel disks at several time points (0h, 1, 7, 14, and 21 days) using fluorescent imaging. By attaching fluorescein dye to gelatin, we assessed its release over time. The results showed that higher amounts of gelatin led to increased and gradual release of gelatin. This release improved the swelling properties, cell viability, and promote bone growth of the hydrogels compared to those made only of MA-alginate. Thus, the gelatin-containing alginate-based bioink developed could effectively support osteogenesis in bone tissue regeneration.
- Journal
- Int J Bioprint. 2023; 9(2): 660.
Active Targeting of Versatile Nanocomplex Using the Novel Biomarker of Breast Cancer Stem Cells
2023
in vivo System
- Title
- Active Targeting of Versatile Nanocomplex Using the Novel Biomarker of Breast Cancer Stem Cells
- Description
- The paper investigates the activation of autophagy through in situ Zn²⁺ chelation reaction to enhance tumor chemoimmunotherapy. The researchers explore how Zn²⁺ chelation can induce autophagy and subsequently improve the efficacy of chemotherapeutic drugs and immune responses against tumors. VISQUE in vivo Smart-LF is used to monitor and analyze the biodistribution of a Zn²⁺ chelator (ZnDPA) in mice. Fluorescent imaging of CT-26 tumor bearing BALB/c mice after 0, 2, 4, 8, 12, 24, 48, and 72h after the intratumoral injection of Cy5.5-labeld DSF@Zn-DMSNs revealed that ZnDPA exhibited significant tumor targeting capability, as evidenced by the accumulation of strong fluorescent signals in the tumor regions. This successful targeting highlights the potential of Zn²⁺ chelation for enhancing the delivery and effectiveness of chemotherapy and immunotherapy in cancer treatment.
- Journal
- Int J Mol Sci. 2023 Jan; 24(1): 685.
Enhancement of the therapeutic efficacy of the MAP regimen using thiamine pyrophosphate‐decorated albumin nanoclusters in osteosarcoma treatment
2023
in vivo System
- Title
- Enhancement of the therapeutic efficacy of the MAP regimen using thiamine pyrophosphate‐decorated albumin nanoclusters in osteosarcoma treatment
- Description
- "Current guidelines for Osteosarcoma recommend combination therapy rather than a single therapy. This study proposed that adding thiamine pyrophosphate (TPP) to nano-drug delivery systems could target bone tumors more effectively without increasing toxicity. The goal was to test whether replacing free methotrexate (MTX) and doxorubicin (DOX) with TPP-coated albumin nanoclusters could improve the effectiveness of the MAP regimen.
VISQUE InVivo Smart was used to monitor fluorescence signals from Cy5.5-labeled nanoclusters injected into mice. Whole-body body fluorescence was tracked using near-infrared fluorescence (NIRF) imaging at various time points: 0, 1, 2, 4, 6, 12, and 24 hours post-injection. After 24 hours, the system was also employed for ex vivo imaging of major organs and tumor tissues. The results showed that the albumin-based nanoclusters gradually accumulated in the osteosarcoma over time, with the TPP-decorated nanoclusters demonstrating improved tumor distributions. Thus, this study showed that the HSA(Human Serum Albumin)-TPP NCs-based MAP regimen may be a promising strategy for Osteosarcoma treatment." - Journal
- Bioeng Transl Med. 2023 Nov; 8(6): e10472.
Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy
2022
in vivo System
- Title
- Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy
- Description
- The study aims to develop and evaluate a novel nanosystem that combines probiotics (Lactobacillus rhamnosus GG) with CRISPR-Cas9 technology to target and treat cancer. The researchers harness the tumor-targeting properties of probiotics and the gene-editing capabilities of CRISPR-Cas9 to specifically attack cancer cells in hypoxic tumor environments, improving the efficacy of cancer therapy. VISQUE imaging system is employed to track and analyze the accumulation and distribution of the nanosystem labeled with cy5.5 within tumor-bearing mice. This fluorescence imaging allowed for real-time observation of how well the nanosystem targeted and accumulated in tumor tissues. The imaging results showed that the hybrid nanosystem (LGG-MHS-Cas9) effectively accumulated in tumor tissues, particularly in hypoxic regions, while showing minimal off-target accumulation in other organs. These findings confirm the nanosystem’s potential for improving the precision and effectiveness of cancer therapies by concentrating the therapeutic agents in the areas where they are most needed.
- Journal
- Nat Commun. 2022; 13: 7903.
Nanorod/nanodisk‐integrated liquid crystalline systems for starvation, chemodynamic, and photothermal therapy of cancer
2022
in vivo System
- Title
- Nanorod/nanodisk‐integrated liquid crystalline systems for starvation, chemodynamic, and photothermal therapy of cancer
- Description
- "This study developed a hybrid gel indocyanine green (ICG), glucose oxidase (GOx), and copper(II) sulfate (Cu) with cellulose nanocrystal (CNC) and Laponite (LAP) was developed for targeted cancer therapy. The gel combines: Photothermal Therapy (PTT), Starvation Therapy (ST), Chemodynamic Therapy (CDT).
VISQUE InVivo Smart was used to evaluate ICG felease form the gel and its uptaken by CT-26 cancer cells, with fluorescence intensity measured at 0, 10, 30, 60, 120, 360, and 1440 min. The CNC/LAP gel with ICG effectively induces hyperthermia in tumors when exposed to near-infrared (NIR) laser, demonstrating improved retention and extended therapeutic effects. Overall, this gel shows promise for localized cancer treatment and maintains a high safety profile." - Journal
- Bioeng Transl Med. 2023 Sep; 8(5): e10470.
Modified exosomal SIRPα variants alleviate white matter injury after intracerebral hemorrhage via microglia/macrophages
2022
in vivo System
- Title
- Modified exosomal SIRPα variants alleviate white matter injury after intracerebral hemorrhage via microglia/macrophages
- Description
- The study aims to develop and evaluate modified exosomal SIRPα variants (SIRPα-v Exos) to alleviate white matter injury after intracerebral hemorrhage (ICH) by promoting the clearance of hematoma and regulating the immune response via microglia/macrophages. VISQUE™ Imaging System was used to monitor the biodistribution of DiR-labeled SIRPα-v Exosomes in mice after intravenous injection. This system tracked the exosomes’ accumulation in various organs over time. Imaging results showed that SIRPα-v Exosomes accumulated in the brain and other organs, with a particularly notable presence in the brain 8 hours post-injection. This confirmed the ability of the exosomes to cross the blood-brain barrier and contribute to hematoma clearance.
- Journal
- Biomater Res. 2022 Nov 26;26:67.
Folate receptor-targeted near-infrared photodynamic therapy for folate receptor-overexpressing tumors
2022
in vivo System
- Title
- Folate receptor-targeted near-infrared photodynamic therapy for folate receptor-overexpressing tumors
- Description
- "Photodynamic therapy (PDT) is a promising minimally invasive cancer treatment that relies on photosensitizers (PS) and light exposure. Developing novel photosensitizers with optimal properties can significantly enhance PDT's effectiveness. Folate receptors (FR), which are overexpressed in about 40% of human cancers, are ideal targets for tumor-specific therapies. By conjugating photosensitizers with folate, specific targeting to FR can be achieved. The folate-linked near-infrared (NIR) probe, FolateSiR-1, has been developed to improve PDT by utilizing NIR light for targeted cancer treatment.
VISQUE InVivo Smart fluorescence imaging and analysis system was used to determine the fluorescence signal intensity (FI) of FilateSiR-1 in tumor-being mice, utilizing a HyperRed light filter set (Ex/Em = 630-680/690-740 nm). This system confirmed high fluorescence in KB tumors but not in OVCAR-3 or A4 tumors, indicating specific binding to folate receptors. Two hours post-injection, the fluorescence intensity peaked in KB tumors and diminished over time. In vivo and ex vivo imaging revealed significant tumor accumulation of FolateSiR-1 and reduced fluorescence in tumors subjected to photodynamic therapy (PDT), compared to untreated tumors. These results support the use of FolateSiR-1 as a promising photosensitizer for targeted PDT with minimal side effects." - Journal
- World J Clin Oncol. 2022 Nov 24; 13(11): 880–895.
ICAM1-Targeting Theranostic Nanoparticles for Magnetic Resonance Imaging and Therapy of Triple-Negative Breast Cancer
2022
in vivo System
- Title
- ICAM1-Targeting Theranostic Nanoparticles for Magnetic Resonance Imaging and Therapy of Triple-Negative Breast Cancer
- Description
- The study aims to design and evaluate ICAM1-targeting theranostic nanoparticles for enhanced magnetic resonance imaging (MRI) and treatment of triple-negative breast cancer (TNBC). The researchers focus on developing nanoparticles that can specifically target TNBC for both imaging and therapeutic purposes. VISQUE InVivo Smart-LF is used to monitor the biodistribution of Cy5.5-labeled nanoparticles in TNBC-bearing mice at various time points (1, 2, 4, and 24 hours post-injection). The imaging showed that the ICAM1-targeting nanoparticles exhibited efficient tumor accumulation compared to the control group. The fluorescence imaging demonstrated prolonged circulation time and enhanced targeting of the nanoparticles to TNBC tumors, confirming their potential for both diagnostic imaging and therapeutic efficacy.
- Journal
- Int J Nanomedicine. 2022 Nov 22;17:5605–5619.
Multiplexed Imaging Reveals the Spatial Relationship of the Extracellular Acidity-Targeting pHLIP with Necrosis, Hypoxia, and the Integrin-Targeting cRGD Peptide
2022
in vivo System
- Title
- Multiplexed Imaging Reveals the Spatial Relationship of the Extracellular Acidity-Targeting pHLIP with Necrosis, Hypoxia, and the Integrin-Targeting cRGD Peptide
- Description
- "The study aimed to investigate the intratumoral distribution (ITD) of pH (low) insertion peptides (pHLIPs) and their spatial relationship with necrosis, hypoxia, and the integrin-targeting cRGD peptide in tumor models.
The VISQUE InVivo Smart-LF was used for in vivo and ex vivo near-infrared fluorescence (NIRF) imaging of tumor-bearing mice. The system helped visualize the distribution of IR800-pHLIP in the tumors at various time points post-injection. The imaging results showed that the ITD of pHLIPs was heterogeneous and had a high degree of colocalization with necrosis. The pHLIPs accumulated in necrotic regions and were spatially contiguous with hypoxia, providing insights into the potential theranostic applications of pHLIPs." - Journal
- Cells. 2022 Nov 4;11(21):3499.
NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma
2022
in vivo System
- Title
- NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma
- Description
- The study aims to explore the role of NRBF2-mediated autophagy in glioblastoma (GBM) cells and how it contributes to metabolite replenishment and radioresistance. The researchers are trying to identify mechanisms through which autophagy can increase GBM cell survival during radiotherapy. With VISQUE Invivo Smart-LF, they perform bioluminescence imaging of tumor growth in GBM-bearing mice models. The researchers monitored tumor growth and assessed the effects of radiation on tumor development. The imaging showed that radiation treatment reduced tumor size, but tumors in the irradiated group exhibited higher expression of NRBF2 and proliferation markers, confirming its role in promoting radioresistance and tumor progression.
- Journal
- Exp Mol Med. 2022 Nov 4;54(11):1872–1885.
Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment
2022
in vivo System
- Title
- Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment
- Description
- "The study aims to investigate the role of shear-wave elasticity (SWE) imaging stiffness in predicting treatment responses for triple-negative breast cancer (TNBC), focusing on how cancer-associated fibroblasts (CAFs) influence tumor elasticity and the effectiveness of therapy.
The Vieworks VISQUE Smart-LF in vivo imaging system was employed to capture SWE images of TNBC tumors, assessing their stiffness as part of the non-invasive imaging technique used to correlate tumor elasticity with clinical outcomes. Imaging with the Vieworks device showed that TNBC tumors had higher SWE stiffness, linked to increased CAF activity, fibrosis, and tumor hypoxia, factors that could potentially predict a tumor's response to neoadjuvant therapies." - Journal
- Bioact Mater. 2022 Nov 3;22:567–587.
HA-DOPE-Modified Honokiol-Loaded Liposomes Targeted Therapy for Osteosarcoma
2022
in vivo System
- Title
- HA-DOPE-Modified Honokiol-Loaded Liposomes Targeted Therapy for Osteosarcoma
- Description
- The paper aims to develop HA-DOPE-modified honokiol-loaded liposomes for targeted osteosarcoma therapy. The researchers seek to enhance the antitumor effects of honokiol (HNK) through improved delivery and targeting of osteosarcoma cells via interaction with the CD44 receptor. Using a VISQUE system, they observed the biodistribution of DiD-labeled liposomes in tumor-bearing mice. The fluorescence images showed that HA-DOPE-modified liposomes had significantly higher accumulation in the tumor compared to non-modified liposomes, demonstrating the effective targeting capability of the liposomal system to osteosarcoma cells via the CD44 receptor.
- Journal
- Int J Nanomedicine. 2022 Nov 1;17:5137–5151.
Small molecule-assisted assembly of multifunctional ceria nanozymes for synergistic treatment of atherosclerosis
2022
in vivo System
- Title
- Small molecule-assisted assembly of multifunctional ceria nanozymes for synergistic treatment of atherosclerosis
- Description
- "The primary objective of the paper is to investigate the potential of multifunctional ceria nanozymes, combined with small molecules, to treat atherosclerosis by simultaneously targeting reactive oxygen species (ROS) and inflammation within atherosclerotic lesions.
The study utilized the Vieworks VISQUE Elit/Smart in vivo imaging system to perform fluorescence imaging, helping visualize the distribution of the nanozymes within atherosclerotic plaques in mice. The result confirmed the effective accumulation of the platelet membrane-coated ceria nanozyme complexes within inflamed atherosclerotic areas, highlighting their targeted delivery and therapeutic potential." - Journal
- Nat Commun. 2022 Nov 1;13:6528.
Lipid Profiles Obtained from MALDI Mass Spectrometric Imaging in Liver Cancer Metastasis Model
2022
in vivo System
- Title
- Lipid Profiles Obtained from MALDI Mass Spectrometric Imaging in Liver Cancer Metastasis Model
- Description
- The paper investigates lipid profiles in liver cancer metastasis using matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) to identify potential biomarkers for metastasis, thereby improving diagnosis and treatment strategies. VISQUE in vivo Elite is used to monitor liver cancer metastasis progression in mice via tracking the localization of SK-Hep1_Luc cells in mouse liver tissues. The imaging results confirmed the accumulation of metastatic lesions in the liver. This allowed researchers to monitor tumor progression and validate the experimental metastasis model.
- Journal
- Int J Anal Chem. 2022 Oct 27;2022:6007158
Exosomal Plasminogen Activator Inhibitor-1 Induces Ionizing Radiation-Adaptive Glioblastoma Cachexia
2022
in vivo System
- Title
- Exosomal Plasminogen Activator Inhibitor-1 Induces Ionizing Radiation-Adaptive Glioblastoma Cachexia
- Description
- "The study aims to elucidate the role of exosomal plasminogen activator inhibitor-1 (PAI-1) in inducing ionizing radiation-adaptive glioblastoma (GBM) cachexia and to explore the potential of targeting PAI-1 as a therapeutic strategy to manage GBM cachexia following radiotherapy.
VISQUE in vivo imaging system Smart-LF was used to monitor xenograft growth by bioluminescent imaging in the orthotopic xenograft mouse model. Results indicated that the total body weight and muscle fiber size of mice decreased after irradiation, indicating the development of cachexia in the irradiated GBM xenograft group." - Journal
- Cells. 2022 Oct 1;11(19):3102.
Huc-MSC-derived exosomes modified with the targeting peptide of aHSCs for liver fibrosis therapy
2022
in vivo System
- Title
- Huc-MSC-derived exosomes modified with the targeting peptide of aHSCs for liver fibrosis therapy
- Description
- The study aims to develop Huc-MSC-derived exosomes modified with a targeting peptide (HSTP1) to specifically target activated hepatic stellate cells (aHSCs) for the treatment of liver fibrosis. The goal is to enhance the delivery efficiency and therapeutic effects of exosomes on reversing aHSC activation and mitigating liver fibrosis. VISQUE in vivo Smart is used to track and analyze the biodistribution of DiR-labeled exosomes in rats. The fluorescence signals in the abdominal liver region are monitored at various time points post-injection. The imaging results showed that the HSTP1-modified exosomes (HSTP1-Exos) exhibited stronger fluorescence signals in the liver, indicating higher targeting efficiency to the aHSC region compared to unmodified exosomes.
- Journal
- J Nanobiotechnology. 2022 Oct 1;20:432.
Effect of Pinoresinol and Vanillic Acid Isolated from Catalpa bignonioides on Mouse Myoblast Proliferation via the Akt/mTOR Signaling Pathway
2022
in vivo System
- Title
- Effect of Pinoresinol and Vanillic Acid Isolated from Catalpa bignonioides on Mouse Myoblast Proliferation via the Akt/mTOR Signaling Pathway
- Description
- This study investigates the effects of pinoresinol and vanillic acid, isolated from Catalpa bignonioides, on mouse myoblast proliferation, aiming to understand their potential in stimulating muscle growth via the Akt/mTOR signaling pathway. VISQUE InVivo Smart-LF was used to capture images of protein expression in Western blot analysis, allowing visualization of signaling pathway activation. Results showed that treatment with pinoresinol and vanillic acid notably increased protein phosphorylation within the Akt/mTOR pathway, supporting muscle cell proliferation and differentiation.
- Journal
- Molecules. 2022 Aug 24;27(17):5397
Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma
2022
in vivo System
- Title
- Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma
- Description
- The study investigates lenvatinib, a multi-kinase inhibitor, as a treatment option for nasopharyngeal carcinoma (NPC) that has developed resistance to antiangiogenic drugs (AADs). It aims to understand the mechanisms of AAD resistance and evaluate lenvatinib’s effectiveness in overcoming this resistance by targeting both VEGFR2 and FGFR1 pathways. VISQUE Invivo Elite is used to detect GFP-positive metastatic nodules in the lungs of NPC-bearing mice, providing detailed insights into tumor growth and metastasis. The imaging showed significant suppression of metastasis in NPC-bearing mice treated with lenvatinib compared to anti-VEGF treatments, confirming lenvatinib's effectiveness in targeting the tumor vasculature and reducing metastasis
- Journal
- Cell Death Dis. 2022 Aug 19;13(8):724.
Reactive oxygen species‐responsive dual‐targeted nanosystem promoted immunogenic cell death against breast cancer
2022
in vivo System
- Title
- Reactive oxygen species‐responsive dual‐targeted nanosystem promoted immunogenic cell death against breast cancer
- Description
- "The study aimed to develop a reactive oxygen species (ROS)-responsive dual-targeted nanosystem to promote immunogenic cell death (ICD) and enhance the therapeutic outcome against breast cancer.
The VISQUE InVivo imaging system was used to evaluate the tumor retention and targeting ability of the nanoparticles in a mouse xenograft model. The imaging results demonstrated that the nanoparticles displayed longer retention in the tumor zone and effectively accumulated in the tumor tissue, indicating their potential for targeted cancer therapy." - Journal
- Bioeng Transl Med. 2022 Aug 3;8(5):e10379.
Cardiomyocytes induced from hiPSCs by well-defined compounds have therapeutic potential in heart failure by secreting PDGF-BB
2022
in vivo System
- Title
- Cardiomyocytes induced from hiPSCs by well-defined compounds have therapeutic potential in heart failure by secreting PDGF-BB
- Description
- The study explores the therapeutic potential of cardiomyocytes induced from human-induced pluripotent stem cells (hiPSCs) using well-defined compounds, focusing on their ability to secrete PDGF-BB and promote myocardial repair in heart failure. VISQUE InVivo Smart-LF was used to perform fluorescence imaging in mice to monitor the biodistribution and homing of transplanted hiPS-CMs over 20 days. The imaging demonstrated that the hiPS-CMs accumulated at the site of injury in the heart, with a longer duration of fluorescent signal observed for the compound-induced hiPS-CMs compared to the standard group, supporting their enhanced therapeutic efficacy.
- Journal
- Signal Transduct Target Ther. 2022 Jul 29;7:253.
Exosomal miR-17-5p from adipose-derived mesenchymal stem cells inhibits abdominal aortic aneurysm by suppressing TXNIP-NLRP3 inflammasome
2022
in vivo System
- Title
- Exosomal miR-17-5p from adipose-derived mesenchymal stem cells inhibits abdominal aortic aneurysm by suppressing TXNIP-NLRP3 inflammasome
- Description
- "The study aimed to investigate the role of exosomal miR-17-5p from adipose-derived mesenchymal stem cells (ADSCs) in inhibiting abdominal aortic aneurysm (AAA) progression by suppressing the TXNIP-NLRP3 inflammasome.
The VISQUE™ InVivo Smart-LF imaging system from Vieworks was used for in vivo fluorescence imaging to track the distribution of DR-labeled ADSC-derived exosomes in mice. Imaging results indicated that the exosomes were primarily localized in the liver, with notable accumulation also seen in the kidneys, lungs, and spleen." - Journal
- Stem Cell Res Ther. 2022 Jul 26;13:349.
Molecular imaging on ACE2‐dependent transocular infection of coronavirus
2022
in vivo System
- Title
- Molecular imaging on ACE2‐dependent transocular infection of coronavirus
- Description
- The study investigates the ACE2-dependent transocular infection pathway of SARS-CoV-2, utilizing molecular imaging to provide dynamic, quantitative insights into how the virus invades and spreads within the body through the eye. VISQUE Smart-LF was employed for ex vivo fluorescence imaging to observe enhanced green fluorescent protein (EGFP) expression, which verified the presence of SARS-CoV-2 in the eyes and brain of treated mice. The imaging demonstrated significant EGFP expression in the eyes and brain of both humanized ACE2 (hACE2) and ACE2 knockout (ACE2-KO) mice, confirming transocular infection.
- Journal
- J Med Virol. 2022 Jul 5;94(10):4878–4889.
Insulin-incubated palladium clusters promote recovery after brain injury
2022
in vivo System
- Title
- Insulin-incubated palladium clusters promote recovery after brain injury
- Description
- The study aimed to synthesize insulin-incubated ultrasmall palladium clusters (Pd@insulin) and evaluate their therapeutic effects on traumatic brain injury (TBI) by scavenging reactive oxygen species (ROS). The VISQUE InVivo Smart-LF was used to trace the distribution of DR-labeled Pd@insulin clusters in mice. The imaging results indicated that Pd@insulin clusters were rapidly distributed throughout the body, with significant accumulation in the liver, kidney, spleen, and brain.
- Journal
- Biomacromolecules. 2022 Apr 22;23(8):3130–3141.
High-Intensity Aerobic Exercise Suppresses Cancer Growth by Regulating Skeletal Muscle-Derived Oncogenes and Tumor Suppressors
2022
in vivo System
- Title
- High-Intensity Aerobic Exercise Suppresses Cancer Growth by Regulating Skeletal Muscle-Derived Oncogenes and Tumor Suppressors
- Description
- The study examines how high-intensity aerobic exercise suppresses cancer growth by regulating skeletal muscle-derived oncogenes and tumor suppressors. It explores the molecular mechanisms behind exercise's anticancer effects using a colorectal cancer mouse model. VISQUE InVivo Smart was used to monitor cancer cell metastasis in mice injected with colorectal cancer cells, enabling the tracking of cancer progression during the study. The in vivo imaging revealed significant tumor growth reduction in mice subjected to high-intensity aerobic exercise, confirming exercise's inhibitory effect on metastasis.
- Journal
- Front Mol Biosci. 2022 Jun 21;9:818470.
Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway
2022
in vivo System
- Title
- Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway
- Description
- "The main goal of the paper is to investigate whether metformin can counteract the senescence of adipose-derived stem cells (ADSCs) and improve outcomes in osteoarthritis (OA) by reducing inflammation and cartilage degradation.
The Vieworks VISQUE in vivo imaging system was used to track the labeled ADSCs injected into the mice to assess their survival and localization in the knee joints at different time points (1, 3, and 7 days post-injection)(Oxidative Medicine and …). The imaging results showed that the fluorescence signal from the DiR-labeled ADSCs persisted up to 7 days, indicating that the cells survived and were active within the joint area during this period(Oxidative Medicine and …)." - Journal
- Oxid Med Cell Longev. 2022 Jun 3;2022:4620254.
Therapeutic strategies targeting uPAR potentiate anti–PD-1 efficacy in diffuse-type gastric cancer
2022
in vivo System
- Title
- Therapeutic strategies targeting uPAR potentiate anti–PD-1 efficacy in diffuse-type gastric cancer
- Description
- This study investigates the potential of targeting the urokinase-type plasminogen activator receptor (uPAR) as a therapeutic approach to enhance anti-PD-1 efficacy in treating diffuse-type gastric cancer (DGC). Smart-LF was utilized to perform bioluminescent imaging on mouse models, allowing researchers to monitor tumor growth and evaluate the effects of the uPAR-targeted therapy. The imaging demonstrated that the combination of anti-uPAR and anti-PD-1 therapies significantly suppressed tumor growth and improved survival rates in the treated mouse models.
- Journal
- Sci Adv. 2022 May 25;8(21):eabn3774.
Quantitative Biodistribution and Pharmacokinetics Study of GMP-Grade Exosomes Labeled with 89Zr Radioisotope in Mice and Rats
2022
in vivo System
- Title
- Quantitative Biodistribution and Pharmacokinetics Study of GMP-Grade Exosomes Labeled with 89Zr Radioisotope in Mice and Rats
- Description
- "The study aimed to assess the quantitative biodistribution and pharmacokinetics of GMP-grade therapeutic exosomes labeled with zirconium-89 (89 Zr) after systemic intravenous administration in mice and rats.
The VISQUE InVivo Smart-LF imaging system was used for in vivo fluorescence imaging to track the distribution of DR-labeled exosomes in mice. The imaging results indicated that the exosomes were mainly distributed in the liver, with significant accumulation also observed in the kidney, lung, and spleen." - Journal
- Pharmaceutics. 2022 May 24;14(6):1118.
FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis
2022
in vivo System
- Title
- FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis
- Description
- The study investigates the impact of FGF-2 signaling on nasopharyngeal carcinoma (NPC) metastasis, focusing on its role in promoting pericyte-macrophage crosstalk to drive tumor spread. VISQUE Invivo Elite was utilized for imaging GFP+ metastatic nodules in a mouse model to assess tumor spread following FGF-2 manipulation. Imaging revealed increased metastatic nodules in FGF-2–overexpressing tumors, suggesting that FGF-2 amplifies tumor metastasis by facilitating pericyte-driven macrophage recruitment
- Journal
- JCI Insight. 2022 May 23;7(10):e157874.
Fluorescence-Shadowing Nanoparticle Clusters for Real-Time Monitoring of Tumor Progression
2022
in vivo System
- Title
- Fluorescence-Shadowing Nanoparticle Clusters for Real-Time Monitoring of Tumor Progression
- Description
- "The study aims to develop a real-time tumor monitoring system using MMP-responsive gold nanoparticle (AuNP) clusters and quantum dots (QD) to detect tumor microenvironments, facilitating better therapeutic strategies.
The VISQUE InVivo Smart-LF system was utilized to visualize fluorescence changes in vivo, confirming the FRET-based quenching of QDs when the AuNPs and QDs formed clusters in response to MMP activity at tumor sites. The imaging results demonstrated significant fluorescence attenuation at tumor sites, which correlated with tumor size and enzyme levels, validating the system’s potential for tumor progression monitoring" - Journal
- Biomacromolecules. 2022 Apr 22;23(8):3130–3141.
Immunotherapeutic effects of recombinant Bacillus Calmette–Guérin containing sic gene in ex vivo and in vivo bladder cancer models
2022
in vivo System
- Title
- Immunotherapeutic effects of recombinant Bacillus Calmette–Guérin containing sic gene in ex vivo and in vivo bladder cancer models
- Description
- The study aims to assess the efficacy of a recombinant Bacillus Calmette-Guérin (rBCG-sic) with enhanced immunotherapeutic effects on bladder cancer, targeting improved response rates in non-muscle invasive bladder cancer. VISQUE In Vivo Smart-LF was utilized to perform bioluminescence imaging to monitor bladder cancer progression in an orthotopic mouse model after rBCG-sic treatment. Imaging results showed that rBCG-sic significantly reduced tumor volume and slowed cancer progression compared to standard BCG, supporting its potential for greater anticancer efficacy.
- Journal
- Investig Clin Urol. 2022 Feb 23;63(2):228–237.
A micellized bone morphogenetic protein-7 prodrug ameliorates liver fibrosis by suppressing transforming growth factor-β signaling
2022
in vivo System
- Title
- A micellized bone morphogenetic protein-7 prodrug ameliorates liver fibrosis by suppressing transforming growth factor-β signaling
- Description
- "The main goal of the paper is to investigate the ability of a micellized bone morphogenetic protein-7 (BMP-7) prodrug to ameliorate liver fibrosis by suppressing transforming growth factor-β (TGF-β) signaling.
The Vieworks VISQUE in vivo Smart imaging system was used to acquire fluorescence images of mice administered with indocyanine green-labeled mPTD-BMP-7 to analyze its distribution in the liver. The imaging results showed that mPTD-BMP-7 was mainly delivered to the liver, indicating efficient hepatic delivery." - Journal
- Am J Cancer Res. 2022 Feb 15;12(2):763–778.
A novel dual-labeled small peptide as a multimodal imaging agent for targeting wild-type EGFR in tumors
2022
in vivo System
- Title
- A novel dual-labeled small peptide as a multimodal imaging agent for targeting wild-type EGFR in tumors
- Description
- The study aims to develop and evaluate a dual-labeled imaging agent, Tc-99m SYPIPDT-ECG-TAMRA, for targeted imaging of tumors that overexpress wild-type epidermal growth factor receptor (wtEGFR). VISQUE In Vivo Smart-LF was employed for ex vivo fluorescence imaging to assess TAMRA-labeled agent uptake in excised tumor and organ tissues from mice. Imaging confirmed higher uptake of the agent in wtEGFR-positive tumors compared to wtEGFR-negative ones, demonstrating the agent's specificity for wtEGFR-positive cells.
- Journal
- PLoS One. 2022 Feb 4;17(2):e0263474
A salicylaldehyde benzoyl hydrazone based near-infrared probe for copper(ii) and its bioimaging applications
2022
in vivo System
- Title
- A salicylaldehyde benzoyl hydrazone based near-infrared probe for copper(ii) and its bioimaging applications
- Description
- This study develops a near-infrared (NIR) fluorescent probe, CySBH, for selective detection and imaging of copper (Cu²⁺) ions, aiming to aid in diagnosing copper-related diseases. VISQUE In Vivo Elite was employed for in vivo imaging to track the probe’s fluorescence response to Cu²⁺ in mice, visualizing its distribution and quenching in real-time. Imaging showed that the probe exhibited strong NIR fluorescence, which decreased with increased Cu²⁺ concentration, confirming the probe’s effective and selective response to Cu²⁺ ions in living subjects.
- Journal
- RSC Adv. 2022 Jan 24;12(5):3073–3080.
Korean Red Ginseng Enhances Immunotherapeutic Effects of NK Cells via Eosinophils in Metastatic Liver Cancer Model
2022
in vivo System
- Title
- Korean Red Ginseng Enhances Immunotherapeutic Effects of NK Cells via Eosinophils in Metastatic Liver Cancer Model
- Description
- This study explores how Korean Red Ginseng (KRG) combined with natural killer (NK) cell therapy enhances the immune response to suppress metastasis in a metastatic liver cancer model. VISQUE In Vivo Elite was used for bioluminescent imaging to track metastasis progression in vivo within the metastatic liver cancer model. The imaging revealed that the combination of KRG and NK cells significantly inhibited metastatic progression, reducing bioluminescence intensity compared to other treatment groups, thereby indicating an enhanced anti-cancer effect.
- Journal
- Nutrients. 2021 Dec 28;14(1):134.
Real-Time Longitudinal Evaluation of Tumor Blood Vessels Using a Compact Preclinical Fluorescence Imaging System
2021
in vivo System
- Title
- Real-Time Longitudinal Evaluation of Tumor Blood Vessels Using a Compact Preclinical Fluorescence Imaging System
- Description
- This study aims to use a compact preclinical fluorescence imaging system to monitor tumor blood vessel dynamics over time, focusing on evaluating the effects of angiogenesis inhibitors in real-time. VISQUE In Vivo Smart-LF was used for time-lapse imaging of tumor vasculature in mice injected with indocyanine green (ICG), allowing for detailed visualization of blood flow and vessel volume changes as the tumor progressed. The imaging revealed that tumor blood flow and vessel volume increased with tumor growth but decreased following angiogenesis inhibitor treatment, demonstrating the system’s utility for monitoring vascular responses to therapy.
- Journal
- Biosensors (Basel). 2021 Nov 23;11(12):471
Local drug delivery using poly(lactic-co-glycolic acid) nanoparticles in thermosensitive gels for inner ear disease treatment
2021
in vivo System
- Title
- Local drug delivery using poly(lactic-co-glycolic acid) nanoparticles in thermosensitive gels for inner ear disease treatment
- Description
- This study aims to develop a localized drug delivery system using poly(lactic-co-glycolic acid) (PLGA) nanoparticles in a thermosensitive gel for treating inner ear diseases, ensuring sustained release of dexamethasone (DEX) directly to the inner ear. VISQUE In Vivo Smart was utilized for in vivo fluorescence imaging to observe the biodistribution and retention of the coumarin-6-loaded PLGA nanoparticles in the inner ear after administration. Imaging revealed that the PLGA nanoparticle-gel formulation remained longer in the middle ear, allowing for a sustained release to the inner ear, as evidenced by prolonged fluorescence intensity up to 48 hours post-administration.
- Journal
- Drug Deliv. 2021 Oct 20;28(1):2268–2277
BEX1 and BEX4 Induce GBM Progression through Regulation of Actin Polymerization and Activation of YAP/TAZ Signaling
2021
in vivo System
- Title
- BEX1 and BEX4 Induce GBM Progression through Regulation of Actin Polymerization and Activation of YAP/TAZ Signaling
- Description
- This study investigates how BEX1 and BEX4 proteins influence glioblastoma (GBM) progression by promoting actin polymerization and activating YAP/TAZ signaling, especially following radiotherapy. VISQUE In Vivo Smart-LF was utilized for bioluminescent imaging to monitor tumor growth and progression in GBM-bearing mouse models following treatments. Imaging demonstrated significant tumor growth inhibition when radiotherapy was combined with Latrunculin B, an actin polymerization inhibitor, indicating potential suppression of GBM aggressiveness.
- Journal
- Int J Mol Sci. 2021 Sep 11;22(18):9845
Downregulated CLIP3 induces radioresistance by enhancing stemness and glycolytic flux in glioblastoma
2021
in vivo System
- Title
- Downregulated CLIP3 induces radioresistance by enhancing stemness and glycolytic flux in glioblastoma
- Description
- The paper aims to explore how downregulation of the protein CLIP3 contributes to radioresistance in glioblastoma by increasing stemness and glycolytic activity, and it investigates the potential of glimepiride, a diabetes drug, as a radiosensitizer to counteract this effect. Smart-LF was used to monitor tumor growth in mice models with orthotopic glioblastoma xenografts after treatment with radiation and glimepiride. The imaging revealed significant inhibition of tumor growth in glioblastoma models treated with a combination of glimepiride and radiation, suggesting an enhanced therapeutic effect compared to radiation alone.
- Journal
- J Exp Clin Cancer Res. 2021 Sep 6;40:282.
Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen
2021
in vivo System
- Title
- Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen
- Description
- The study aims to develop an adjuvant formulation combining DOTAP, CpG D35, and aluminum salt to enhance both T cell and antibody responses to protein antigens, thereby improving vaccine efficacy for infections and cancer. VISQUE in vivo Smart-LF was used to track antigen distribution in vivo, particularly focusing on how the adjuvant formulation influences antigen retention at the injection site and migration to lymph nodes. The system showed that the DOTAP-containing formulation retained antigens at the injection site and increased antigen presence in draining lymph nodes over time, suggesting improved delivery and immune response initiation.
- Journal
- PLoS One. 2021 Aug 2;16(8):e0254628
CKAP4 Antibody-Conjugated Si Quantum Dot Micelles for Targeted Imaging of Lung Cancer
2021
in vivo System
- Title
- CKAP4 Antibody-Conjugated Si Quantum Dot Micelles for Targeted Imaging of Lung Cancer
- Description
- The paper investigates the use of CKAP4 antibody-conjugated silicon quantum dot micelles (Si QD micelles-CKAP4) as a targeted imaging agent for lung cancer, aiming to improve fluorescence-guided surgery. Smart-LF was utilized for in vivo fluorescence imaging to track the biodistribution and targeting efficacy of Si QD micelles-CKAP4 in tumor-bearing mice. The imaging results showed strong fluorescence in lung cancer tissues compared to healthy tissues, confirming the Si QD micelles-CKAP4’s potential for specific targeting in lung cancer imaging.
- Journal
- Nanoscale Res Lett. 2021 Jul 31;16:124.
Enzyme-Loaded pH-Sensitive Photothermal Hydrogels for Mild-temperature-mediated Combinational Cancer Therapy
2021
in vivo System
- Title
- Enzyme-Loaded pH-Sensitive Photothermal Hydrogels for Mild-temperature-mediated Combinational Cancer Therapy
- Description
- The study aims to create a glucose oxidase-loaded, pH-sensitive photothermal hydrogel that can combine mild-temperature photothermal therapy (PTT) and starvation therapy to treat solid tumors effectively and safely. VISQUE in vivo Smart-LF was utilized to capture bioluminescence images, assessing the anti-metastasis effects of the hydrogels by measuring bioluminescence intensity in lung tissues. Imaging with the system revealed significantly reduced lung bioluminescence signals in mice treated with the hydrogel under laser irradiation, indicating effective inhibition of tumor metastasis to the lungs.
- Journal
- Front Chem. 2021 Jul 29;9:736468.
Artificially engineered antiferromagnetic nanoprobes for ultra-sensitive histopathological level magnetic resonance imaging
2021
in vivo System
- Title
- Artificially engineered antiferromagnetic nanoprobes for ultra-sensitive histopathological level magnetic resonance imaging
- Description
- The study aims to develop ultra-sensitive antiferromagnetic nanoparticle probes for high-resolution, non-invasive magnetic resonance imaging (MRI) at the histopathological level, with applications for detecting microscopic tumors and micrometastases. VISQUE InVivo Elite was used to capture fluorescence images of liver tissues in mice post-MRI to validate tumor targeting and distribution of the nanoprobe. Imaging with the VISQUE system confirmed specific targeting and strong fluorescence signals within microscopic tumors, corroborating the MRI findings and illustrating the probes' effectiveness in detecting small-scale tumors.
- Journal
- Nat Commun. 2021 Jun 22;12:3840
Marriage of Virus‐Mimic Surface Topology and Microbubble‐Assisted Ultrasound for Enhanced Intratumor Accumulation and Improved Cancer Theranostics
2021
in vivo System
- Title
- Marriage of Virus‐Mimic Surface Topology and Microbubble‐Assisted Ultrasound for Enhanced Intratumor Accumulation and Improved Cancer Theranostics
- Description
- The study aims to improve intratumor accumulation and therapeutic efficacy of nanoparticles for cancer treatment by combining virus-mimic surface topology with microbubble-assisted ultrasound. VISQUE in vivo Elite was used to conduct in vivo fluorescence imaging, selecting the ICG channel to assess nanoparticle accumulation in the tumor. The system successfully detected enhanced fluorescence intensity in tumors after treatment, demonstrating increased intratumor accumulation due to the combined nanoparticle design and ultrasound strategy.
- Journal
- Adv Sci (Weinh). 2021 May 14;8(13):2004670.
In Vitro Photodynamic Effects of the Inclusion Nanocomplexes of Glucan and Chlorin e6 on Atherogenic Foam Cells
2021
in vivo System
- Title
- In Vitro Photodynamic Effects of the Inclusion Nanocomplexes of Glucan and Chlorin e6 on Atherogenic Foam Cells
- Description
- The study investigated the in vitro photodynamic effects of Glucan/Chlorin e6 (Glu/Ce6) nanocomplexes on atherogenic foam cells as a potential treatment for atherosclerosis. The researchers used VISQUE in vivo Smart-LF to capture fluorescence images of the Glu/Ce6 nanocomplexes and their singlet oxygen generation under laser irradiation. The imaging showed successful internalization of the Glu/Ce6 nanocomplexes in foam cells, confirming efficient delivery of the photosensitizer Ce6 to targeted cells and demonstrating high singlet oxygen generation upon laser exposure.
- Journal
- Int J Mol Sci. 2020 Dec 26;22(1):177
Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer
2021
in vivo System
- Title
- Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer
- Description
- Trastuzumab is the main component of HER2-directed gastric cancer therapy, but patient response is poor due to insufficient cell sensitivity and drug resistance. In this paper, the researchers report that HER2 is involved in cell mitotic promotion for tumorigenesis and a poor response of patients to trastuzumab therapy by hyperactivating a crucial HER2-SHCBP1-PLK1 axis. Theaflavine-3, 3’-digallate (TFBG), an inhibitor of the SHCBP1-PLK1 interaction, acts as a potential trastuzumab sensitizer and shows high efficacy in inhibiting the growth of HER2-positive gastric cancer when combined with trastuzumab. The longitudinal response of NCI-N87 tumor to trastuzumab is observed by in vivo luminescence imaging with Visque InVivo Smart-LF after the treatment of trastuzumab with knockdown of SHCBP1 or TFBG treatment on NCI-N87/luc tumor xenograft model mice.
- Journal
- Nature Communications 2021 May 14;12(1):2812
Lung-selective 25-hydroxycholesterol nanotherapeutics as a suppressor of COVID-19-associated cytokine storm
2021
in vivo System
- Title
- Lung-selective 25-hydroxycholesterol nanotherapeutics as a suppressor of COVID-19-associated cytokine storm
- Description
- Decreased 25-hydroxycholesterol (25-HC) and increased cytokine levels is believed to play a crucial role in COVID-19 pathogenesis, inducing acute respiratory distress syndrome (ARDS) and sepsis. In this paper, the researchers develop 25-HC and didodecyldimethylammonium bromide (DDAB) nanovesicles (25-HC@DDAB) as a COVID-19 drug candidate for the restoration of intracellular cholesterol level and suppression of cytokine storm in severe SARS-CoV-2 patients. 25-HC@DDAB can selectively accumulate the lung tissues and effectively reduce inflammatory cytokine levels in COVID-19 patient-derived PBMCs. The researchers confirm the accumulation of 25-HC@DDAB in lung by ex vivo fluorescence imaging with Visque InVivo Smart at the specific time point after intravenous injection of 25-HC@DDAB into the tail vein of mice.
- Journal
- Nano Today 2021 Jun; 38: 101149 2021 Jan 11;
RNA interference-mediated suppression of TNF-α converting enzyme as an alternative anti-TNF-α therapy for rheumatoid arthritis
2021
in vivo System
- Title
- RNA interference-mediated suppression of TNF-α converting enzyme as an alternative anti-TNF-α therapy for rheumatoid arthritis
- Description
- For approximately 90% of patients with RA, anti-TNF-α therapy is treated as the first-line immuno-treatment since excessive tumor necrosis factor-α (TNF-α) is associated with the pathogenesis of rheumatoid arthritis (RA). In this paper, researchers suggested suppression of TNF-α converting enzyme (TACE) as a novel strategy to down-regulate TNF-α level. They developed a disease site-targeted RNA interference system by forming non-viral complex between shRNA against TACE (shTACE) and bone resorption site-specific peptide carrier. In collagen induced arthritis (CIA) model experiments, the shTACE/peptide carrier complex alleviated arthritic symptoms with enhanced anti-inflammatory and anti-osteoclastogenic effects. And, similar results were obtained with human origin cell experiments from tissues and synovial fluids of RA patients. The biodistribution of shTACE/peptide carrier complex was identified by in vivo fluorescence imaging with Visque InVivo Smart after intravenous injection of shTACE/peptide carrier complex into CIA model mice.
- Journal
- Journal of Controlled Release 2021 Feb 10;330:1300-1312
Fenton-like reaction, glutathione reduction, and photothermal ablation-built-in hydrogels crosslinked by cupric sulfate for loco-regional cancer therapy
2021
in vivo System
- Title
- Fenton-like reaction, glutathione reduction, and photothermal ablation-built-in hydrogels crosslinked by cupric sulfate for loco-regional cancer therapy
- Description
- In this paper, the researchers developed HC/Cu/ICG hydrogel using crosslinked hydrogel systems to apply Fenton-like reaction-associated chemodynamic therapy (CDT) and hyperthermia-inducing photothermal therapy (PTT) for loco-regional cancer therapy. HC/Cu/ICG hydrogel was developed by incorporating Cupric sulfate (Cu), catechol-functionalized hyaluronic acid (HC) and indocyanine green (ICG). HC/Cu/ICG hydrogel showed therapeutic efficiency in the loco-regional CDT and PTT of cancers while not inducing any severe systemic toxicities in in vivo experiments. The Biodegradability of HC/Cu/ICG hydrogel was investigated by in vivo fluorescence imaging with Visque InVivo Smart at the specific days after the subcutaneous injection of HC/Cu/ICG hydrogel into mice.
- Journal
- Biomaterials Science 2021 Feb 7;9(3):847-860
Dissecting the impact of target-binding kinetics of protein binders on tumor localization
2021
in vivo System
- Title
- Dissecting the impact of target-binding kinetics of protein binders on tumor localization
- Description
- To improve the therapeutic effects of protein-based therapeutics, their in vivo behavior is considered and controlled systematically. In this paper, the researchers investigate the influence of the binding affinity of proteins on tumor localization by using four repebodies having different affinities to EGFR on cancer model mice. They find that optimal affinity with balanced target binding and dissociation can facilitate deep penetration and accumulation of protein binders in tumors by overcoming the binding-site-barrier effect. The BioDistribution of repebodies in the body is identified and quantitated by in vivo fluorescence imaging with Visque InVivo Smart-LF at specific time points after the intravenous injection of various repebody into A431 tumor xenograft model mice.
- Journal
- iScience 2021 Feb 19; 24(2): 102104.
Antibreast Cancer Activity of Aspirin-Conjugated Chalcone Polymeric Micelles
2021
in vivo System
- Title
- Antibreast Cancer Activity of Aspirin-Conjugated Chalcone Polymeric Micelles
- Description
- In this paper, the researchers developed new aspirin-conjugated chalcone derivative-loaded nanoparticle (AS-DK143-loaded NP) using mPEG-PLA co-polymer. AS-DK143 NP showed the reduction of cell viability of 4T1 cells in vitro and anti-cancer efficacy with the selective targetability on cancer and the reduction of tumor size in 4T1-luc cell bearing mice. The BioDistribution of AS-DK143 NP in the body is identified by ex vivo fluorescence imaging of the extracted tissues with Visque InVivo Smart at specific time points after the subcutaneous injection into 4T1 xenograft mice.
- Journal
- Macromolecular Research 2021 Jan 28;29:105–110
Meso-pyridinium substituted BODIPY dyes as mitochondria-targeted probes for the detection of cysteine in living cells and in vivo
2021
in vivo System
- Title
- Meso-pyridinium substituted BODIPY dyes as mitochondria-targeted probes for the detection of cysteine in living cells and in vivo
- Description
- Cysteine (Cys) is a bio-thiol acting as an antioxidant against ROS which can cause oxidative damage in mitochondria. The researchers develop BDP-S-o-Py+ as fluorescent probes by substition of and meso-pyridinium of BODIPY, which is a mitochondria-targeted probe for Cys-specific detection. BDP-S-o-Py+ can detect mitochondrial Cys in living cells in vito, and endogenous Cys in vivo. BDP-S-o-Py+ showed satisfactory capability to image Cys in vivo in in vivo fluorescence imaging with Visque InVivo Smart after the subcutaneous injection of BDP-S-o-Py+ and Cys into mouse.
- Journal
- Dyes and Pigments 2021 Mar;187:109089
Characterization of adipose-derived stromal/stem cell spheroids versus single-cell suspension in cell survival and arrest of osteoarthritis progression
2021
in vivo System
- Title
- Characterization of adipose-derived stromal/stem cell spheroids versus single-cell suspension in cell survival and arrest of osteoarthritis progression
- Description
- In this paper, researchers tested spheroidal adipose-derived stromal/stem cells (ASCs) for the treat of surgically induced osteoarthritis (OA) in a rat model. ASC spheroids cultured in 3D have better in vitro and in vivo cell survival and chondrogenic potential, resulting in better regeneration and the arrest of surgically induced OA than ASCs in single-cell suspension. ASCs in the knee joints of rats with surgically induced OA were tracked by fluorescence in vivo imaging with Visque InVivo Smart at the specific time point after the intra-articular injection of ASCs.
- Journal
- Journal of Biomedical Materials Research Part A 2021 Jun;109(6):869-878
Optically activatable photosynthetic bacteria-based highly tumor specific immunotheranostics
2020
in vivo System
- Title
- Optically activatable photosynthetic bacteria-based highly tumor specific immunotheranostics
- Description
- In this paper, the non-pathogenic natural purple photosynthetic bacteria (PPSB) is investigated as bacteria for bacterial therapy in solid tumor treatment. PPSB exhibit highly targeted tumor elimination and precisely marking tumor location by showing strong NIR-I-to-NIR-II reporter fluorescence, powerful photothermal conversion, excellent reactive oxygen species generation, and contrasting photo-acoustic effects. The optical efficacies of the bacterial treatment is dramatically improved with short-term fasting. The tumor targetability of PPSB is investigated with the biodistribution of PPSB by in vivo fluorescence imaging and ex vivo fluorescence imaging with Visque InVivo Smart-LF at specific time points after intravenous injection of PPSB into Colon26 tumor-bearing mice.
- Journal
- Nano Today 2020 Apr;37:101100
Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta–Mediated Epithelial–Mesenchymal Transition
2020
in vivo System
- Title
- Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta–Mediated Epithelial–Mesenchymal Transition
- Description
- Protein transduction domain (PTD) fused BMP-7 in micelle (mPTD-BMP-7) is developed as the effective therapeutics for renal fibrosis. mPTD-BMP-7 successfully inhibits the TGF-β–mediated epithelial–mesenchymal transition process in vitro and in an in vivo unilateral ureter obstruction model. The researchers identified the clinical relevance of mPTD-BMP-7 by developing and applying an intra-arterial administration of mPTD-BMP-7 through renal artery in pigs. mPTD-BMP-7 through renal artery intervention effectively delivered into Bowman’s space and inhibits unilateral ureter obstruction–induced renal fibrosis in pigs. The biodistribution of mPTD-BMP-7 in the kidney was observed using ex vivo fluorescence imaging of the kidney with Visque InVivo Smart at specific time points after the intra-arterial administration of mPTD-BMP-7 through renal artery.
- Journal
- Frontiers in Pharmacology 2020 Nov 19;11:591275
Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer
2020
in vivo System
- Title
- Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer
- Description
- In this paper, researchers developed a biophotonic nanoplatform by integrating the zirconium carbide (ZrC) nanosheet as a deep PTT-photosensitizer and on-demand designed anticancer prodrug SN38-Nif. The integrated ZrC@prodrug biophotonic nanoplatform shows the potent anticancer effects in in vitro and in vivo experiments. In mouse models, the ZrC@prodrug system markedly inhibits tumor recurrence, metastasis, inflammation and angiogenesis. The synergistic inhibition of tumor growth with ZrC@prodrug was confirmed by ex vivo fluorescence imaging of tumors in liver with Visque InVivo Elite at a specific time point after the treatment on mouse model.
- Journal
- Advanced Science 2020 Nov 5;7(24):2001191
Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis
2020
in vivo System
- Title
- Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis
- Description
- The bone morphogenetic protein-7 (BMP-7) is a therapeutic growth factor reverting many fibrotic diseases, including peritoneal fibrosis by peritoneal dialysis (PD). The researcher developed a novel drug delivery system using protein transduction domains (PTD) and made PTD-BMP-7 by applying it on BMP-7. In in vivo study using rats, PTD-BMP-7 showed the therapeutic effects on epithelial-mesenchymal transition (EMT)-related fibrosis by restoring the changes of peritoneum and suppress the progress of established PD fibrosis. The researchers investigated the biodistribution and the half-life of PTD-BMP-7 by fluorescence in vivo imaging with Visque InVivo Elite and fluorescence ex vivo imaging with Visque InVivo Smart at the specific time point after the intra-peritoneal injection of PTD-BMP-7 into mice.
- Journal
- Journal of Cellular and Molecular Medicine 2020 Nov;24(22):13507-13522
“Star” miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer
2020
in vivo System
- Title
- “Star” miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer
- Description
- In this paper, DPC/miR-34a nanoplex was developed by self-assembly of Dextrin modified 1.8 k PEI with CM-end (Dextrin-PEI-CM, DPC) and Star miR-34a, a cell adhesion molecules CD44 inhibitor. DPC/miR-34a nanoplex showed good ability in suppression of cancer cell invasion in an in vitro cell invasion assay and in vivo anti-metastasis model. And, DPC/miR-34a demonstrated a superior antitumor and anti-metastatic efficacy both in lung metastatic model and orthotopic MDA-MB-231 tumor models. The biodistribution of DPC/miR-34a was identified by fluorescence in vivo and ex vivo imaging with Visque InVivo Elite at the specific time points after the injection of DPC/miR-34a into the tail vein of MDA-MB-231 tumor model mice.
- Journal
- Journal of Controlled Release 2020 Oct 10;326:615-627
Ferritin Nanocage-Based Methyltransferase SETD6 for COVID-19 Therapy
2020
in vivo System
- Title
- Ferritin Nanocage-Based Methyltransferase SETD6 for COVID-19 Therapy
- Description
- In this paper, the ferritin nanocage-based peptide delivery system was used for the delivery of cell-penetrating peptide and nuclear-localizing TAT-NBD peptide with SETD6. TAT NBD-short ferritin-SETD6 (TFS) enhances the SETD6 level and reduces the nuclear factor-κB (NF-κB) signaling. The TFS make the survival rate of cytokine storm model mouse better and the lung tissue damage and cytokine expression reduced. The biodistribution of TFS in cytokine storm model mouse was observed by fluorescence ex vivo imaging of organs with Visque InVivo Smart at the specific time point after the i.v. injection of TFS into mouse body.
- Journal
- Advanced Functional Materials 2020 Nov 25;30(48):2006110
A brain tumor-homing tetra-peptide delivers a nano-therapeutic for more effective treatment of a mouse model of glioblastoma
2020
in vivo System
- Title
- A brain tumor-homing tetra-peptide delivers a nano-therapeutic for more effective treatment of a mouse model of glioblastoma
- Description
- Organ-specific cell-penetrating peptides (CPPs) are a kind of molecules that can deliver drugs very effectively, and they are currently of great interest in cancer treatment strategies. In this paper, the researchers developed amino acid sequence serine-isoleucine-tyrosine-valine(SIWV) as a new CPPs to target glioblastoma multiforme (GBM) brain tumor tissues. In in vitro and in vivo experiments, enhanced in vivo targeting ability of SIWV was showed. The biodistribution and capability as a targeting agents were confirmed by fluorescence in vivo imaging and ex vivo imaging with Visque InVivo Smart-LF at specific time points after the injection of SIWV linked with SN-38, the anticancer drug, into the body of GBM xenograft mice.
- Journal
- Nanoscale Horizons 2020 Jul 27;5(8):1213-1225
Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature
2020
in vivo System
- Title
- Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature
- Description
- In this paper, the researchers develop 2 jet-lagged nanoparticles, apatinib (APA)-loaded TEC-targeting nanodrug (APA/MCP) for vascular normalization therapy and lonidamine (LND)-loaded tumor cell-targeting nanodrug (LND/MCA) for tumor cell metabolic treatment. APA/MCP can block VEGF/VEGFR2 to inhibit tumor endothelial cells (TECs) proliferation and LND/MCA can inhibit lactic acid (LA) efflux to remodel tumor acid metabolism. Moreover, the results of in vivo pharmacodynamic studies show that the therapeutic effect of programmed death 1 (PD-1) drug with jet-lagged nanoparticles is improved 3-folds to that of the PD-1 group. Tumor targetability and distribution behavior of Jet-lagged nanoparticles were identified by fluorescence in vivo imaging and ex vivo imaging with Visque InVivo Elite at specific time points after the injection of Jet-lagged nanoparticles into B16F10 bearing mice.
- Journal
- Advanced Healthcare Materials 2020 Jun;9(12):e2000075
Dual functional nanoparticles containing SOX duo and ANGPT4 shRNA for osteoarthritis treatment
2020
in vivo System
- Title
- Dual functional nanoparticles containing SOX duo and ANGPT4 shRNA for osteoarthritis treatment
- Description
- In this study, the researchers designed nanoparticles comprising dexamethasone-conjugated polyethylenimine (DEXPEI) complexed with minicircle plasmid (MC) harboring SOX duo (SOX-9, -6) and ANGPTL4 small hairpin RNA (shANG) [MCSOX9/6/shANG] to enhance chondrogenesis of stem cells and suppress inflammation in OA. Adipose-derived stem cells (ADSCs) transfected with MCSOX9/6/shANG (MCSOX9/6/shANG-tADSCs) showed enhanced chondrogenesis and suppressed progression of osteoarthritis (OA) in both in vitro and in vivo experiments. Fluorescence labeled MCSOX9/6/shANG-tADSCs were tracked by in vivo fluorescence imaging with Visque InVivo Elite at specific time points after injection of MCSOX9/6/shANG-tADSCs into right knee joint of rats in which OA had been surgically induced.
- Journal
- Journal of Biomedical Materials Research Part B: Applied Biomaterials 2020 Jan;108(1):234-242
Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery
2020
in vivo System
- Title
- Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery
- Description
- In this paper, the researchers suggest a noninvasive active-targeting therapy for ischemic stroke using a neutrophil-like cell-membrane-coated mesoporous Prussian blue nanozyme (MPBzyme@NCM). Despite its robust anti-inflammatory and antioxidative stress properties, nanozyme has shown unsatisfactory therapeutic effects due to its insufficient accumulation in the ischemic brain via noninvasive administration. The researchers improve its delivery to the lesion site by coating the nanozyme with the neutrophil-like membrane. They also demonstrate the long-term in vivo therapeutic efficacy for ischemic stroke after the delivery into the damaged brain. The in vivo fluorescence imaging with Visque InVivo Elite is used to confirm the accumulation of MPBzyme@NCM at the lesion site over time.
- Journal
- ACS Nano
Interrogation of Folic Acid-Functionalized Nanomedicines: The Regulatory Roles of Plasma Proteins Reexamined
2020
in vivo System
- Title
- Interrogation of Folic Acid-Functionalized Nanomedicines: The Regulatory Roles of Plasma Proteins Reexamined
- Description
- Folic acid (FA) has been widely used to promote targeted delivery of nanomedicines to tumors since the folate receptor-α (FR-α) is overexpressed in many human cancers. In this paper, the researchers find that the FA functionalization induces heavy natural IgM absorption on the liposomal surface, preventing FA from binding receptors and accelerating complement activation in vivo. In addition, FA-functionalized polymeric nanoparticles are found to be vulnerable to natural IgM absorption. The biodistribution of FA-functionalized liposome (FA-sLip) at specific time points after the tail vein is observed using in vivo fluorescence imaging with Visque InVivo Elite.
- Journal
- ACS Nano
Supramolecular Container-Mediated Surface Engineering Approach for Regulating the Biological Targeting Effect of Nanoparticles
2020
in vivo System
- Title
- Supramolecular Container-Mediated Surface Engineering Approach for Regulating the Biological Targeting Effect of Nanoparticles
- Description
- In this paper, researchers suggest a supramolecular container-based surface engineering approach to make a chemical bridge to incorporate nanoparticles and targeting ligands. The researchers use an acyclic cucurbituril (aCB) molecular container as a supramolecular container to incorporate nanoparticles and targeting ligands via a bilateral host-guest complexation, enabling better water dispersibility and enhanced biological targeting. The aCB-engineered nanoparticles show the enhanced biological targeting effect and targeted imaging performance in in vitro and in vivo experiments. The enhanced targeting and imaging performance are demonstrated by the fluorescence imaging of tumor-bearing mice with Visque InVivo Elite at the specific time point after intravenous injection of rhodamine B isothiocyanate (RITC)-labeled nanoparticle.
- Journal
- Nano Letters
Enhancement of Nanozyme Permeation by Endovascular Interventional Treatment to Prevent Vascular Restenosis via Macrophage Polarization Modulation
2020
in vivo System
- Title
- Enhancement of Nanozyme Permeation by Endovascular Interventional Treatment to Prevent Vascular Restenosis via Macrophage Polarization Modulation
- Description
- In this paper, the researchers examine nanozymes as an effective therapeutic strategy for vascular restenosis after endovascular interventional treatment. Nanozymes are excellent at scavenging ROS, the major aggravating factor for vascular restenosis, but cannot efficiently pass through the vascular endothelial cell barrier to reach the vascular intima. The researchers discover that the endothelial cells exfoliation by endovascular interventional treatment enhances nanoparticle permeation into the vascular intima and uptake by macrophages to alleviate long-term vascular restenosis in vivo. The vascular restenosis is relieved after vascular balloon injury (VBI) under the presence of Prussian blue nanozyme (PBzyme). The accumulation of PBzyme in left common carotid arteries of mice is confirmed with in vivo fluorescence imaging using Visque InVivo Elite.
- Journal
- Advanced Functional Materials
A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues
2020
in vivo System
- Title
- A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues
- Description
- In this paper, the researchers develop a specific substrate for Acetylcholinesterase (AChE) detection by combining dimethylcarbamate choline with a self-immolative scaffold. Imaging the activity of AChE which is tightly associated with neurological diseases is difficult due to the interference from butyrylcholinesterase (BChE) and carboxylesterase (CE). The representative substrate P10 can effectively eliminate the interference from CE and BChE. Its high specificity is confirmed via imaging AChE activity in vitro. Also, the AChE activity in a normal mouse brain is successfully captured via in vivo and ex vivo fluorescence imaging with Visque InVivo Elite.
- Journal
- Chemical Science
Molecular Design of Conjugated Small Molecule Nanoparticles for Synergistically Enhanced PTT/PDT
2020
in vivo System
- Title
- Molecular Design of Conjugated Small Molecule Nanoparticles for Synergistically Enhanced PTT/PDT
- Description
- In this paper, IID-ThTPA nanoparticles (IID-ThTPA NPs) are proposed as a new phototheranostic agent for simultaneous photothermal therapy (PTT) and photodynamic therapy (PDT). The researchers design a donor–acceptor–donor (D–A–D) conjugated small molecule (IID-ThTPA)-based phototheranostic agent, with isoindigo (IID) as the selective acceptor and triphenylamine (TPA) as the donor. This IID-ThTPA shows superior tumor cooperative eradicating capability in vitro and in vivo with a high PTT/PDT performance. The BioDistribution of IID-ThTPA nanoparticles in the body is identified by ex vivo fluorescence imaging of the extracted tissues with Visque InVivo Elite at specific time points after the intravenous injection.
- Journal
- Nano-Micro Letters
In Vivo Imaging of Senescent Vascular Cells in Atherosclerotic Mice Using a β-Galactosidase-Activatable Nanoprobe
2020
in vivo System
- Title
- In Vivo Imaging of Senescent Vascular Cells in Atherosclerotic Mice Using a β-Galactosidase-Activatable Nanoprobe
- Description
-
Monoiodo Aza-BODIPY is developed as a new Near-Infrared Photosensitizer (PS) for Photodynamic therapy (PDT), which is a rising platform of the cancer treatment method, and its antitumor efficacy is demonstrated both in vivo and in vitro.
BODIPYs possess many advantages of ideal PS characteristics and aza-BODIPYs among them have large absorption coefficient and NIR absorption to fit the therapeutic window (650 - 900nm). The researchers make many substitution variations on aza-BODIPY and find that monoiodo derivatives of aza-BODIPY have various advantages of an ideal PS with excellent photostability, chemical stability, and photocytotoxicity. The superior antitumor efficacy of Monoiodo Aza-BODIPY is demonstrated both in vitro and in vivo.
The biodistribution of Monoiodo Aza-BODIPY at specific time points after the tail vein injection is explored using in vivo and ex vivo fluorescence imaging of mice with Visque InVivo Smart. - Journal
- Analytical Chemistry
Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy
2020
in vivo System
- Title
- Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy
- Description
-
Monoiodo Aza-BODIPY is developed as a new Near-Infrared Photosensitizer (PS) for Photodynamic therapy (PDT), which is a rising platform of the cancer treatment method, and its antitumor efficacy is demonstrated both in vivo and in vitro.
BODIPYs possess many advantages of ideal PS characteristics and aza-BODIPYs among them have large absorption coefficient and NIR absorption to fit the therapeutic window (650 - 900nm). The researchers make many substitution variations on aza-BODIPY and find that monoiodo derivatives of aza-BODIPY have various advantages of an ideal PS with excellent photostability, chemical stability, and photocytotoxicity. The superior antitumor efficacy of Monoiodo Aza-BODIPY is demonstrated both in vitro and in vivo.
The biodistribution of Monoiodo Aza-BODIPY at specific time points after the tail vein injection is explored using in vivo and ex vivo fluorescence imaging of mice with Visque InVivo Smart. - Journal
- Journal of Medicinal Chemistry
2020 Sep 10;63(17):9950-9964
Detecting Cysteine in Bioimaging with a Near‐Infrared Probe Based on a Novel Fluorescence Quenching Mechanism
2020
in vivo System
- Title
- Detecting Cysteine in Bioimaging with a Near‐Infrared Probe Based on a Novel Fluorescence Quenching Mechanism
- Description
-
The highly selective and sensitive NIR turn-on fluorescent probe (BDP-NIR) was developed to detect cysteine (Cys) in biological systems.
BDP-NIR was based on BODIPY with a large Stokes shift (105 nm) when detecting Cys. BDP-NIR had a high linear dynamic range from 0 μM to 500 μM, which was promising for detecting of Cys quantificationally. The researchers took fluorescence imaging of mice with Visque InVivo Elite to evaluate the capability of sensing endogenous Cys in vivo. - Journal
- ChemBioChem
Heme Oxygenase 1-Targeted Hybrid Nanoparticle for Chemo- and Immuno-Combination Therapy in Acute Myelogenous Leukemia
2020
in vivo System
- Title
- Heme Oxygenase 1-Targeted Hybrid Nanoparticle for Chemo- and Immuno-Combination Therapy in Acute Myelogenous Leukemia
- Description
-
The new hybrid nanoparticle is developed as a novel therapeutic in acute myelogenous leukemia (AML) and its potential was assessed with a human AML-bearing orthotopic mouse model.
The lipid-polymer hybrid nanoparticle (hNP) is loaded with tin mesoporphyrin (SnMP), an inhibitor of Heme Oxygenase 1 (HO1) which is an antioxidative and cytoprotective enzyme inducing chemo-resistant AML, and an engineered antibody for leukemic cell-targeted delivery. HO1-inhibiting T-hNP (T-hNP/SnMP) enhances chemo-sensitivity in human leukemia cells. The T-hNP/SnMP enhances the chemo-therapeutic effect of daunorubicin and boosts immune response by reprogramming bone marrow myeloid cells.
In the ex vivo study, the researchers identify the organ distribution of T-hNP/SnMP at specific time points after the intravenous injection and confirm the presence of T-hNP/SnMP in the bone marrow by fluorescence imaging of mice with Visque InVivo Smart. - Journal
- Advanced Science
2020 Jul; 7(13): 2000487
Penta-fluorophenol: A Smiles rearrangement-inspired cysteine-selective fluorescent probe for imaging of human glioblastoma
2020
in vivo System
- Title
- Penta-fluorophenol: A Smiles rearrangement-inspired cysteine-selective fluorescent probe for imaging of human glioblastoma
- Description
- The electron-deficient penta-fluorophenol (NPO-B) was developed as a sensing fluorescence probe to measure the cysteine (Cys) level, which is a biomarker of glioblastomoa (GBM). The probe provided a significant fluorescence response with high selectivity, high sensitivity, a fast response time. The researcher identified that this probe can be used for a relatively simple diagnosis and the tracking of GBM at clinical sites by ex vivo fluorescence imaging in both mouse models and human tissue samples with Visque InVivo Elite.
- Journal
- Chemical Science
2020 May 11;11(22):5658-5668
Selenium and dopamine-crosslinked hyaluronic acid hydrogel for chemophotothermal cancer therapy
2020
in vivo System
- Title
- Selenium and dopamine-crosslinked hyaluronic acid hydrogel for chemophotothermal cancer therapy
- Description
- Sodium selenite (Se)-directed crosslinked hydrogels based on hyaluronic acid (HA)-dopamine (HD), including indocyanine green (ICG), were developed for local cancer therapy. This HD/Se/ICG gel showed easy injectability, slow biodegradability and sufficient photothermal efficiencies with NIR laser irradiation, providing efficient tumor growth inhibition capability without severe systemic toxicities. The biodegradability of the gel in the body was tested by in vivo fluorescence imaging of mice with Visque InVivo Smart at specific time points after the intratumoral injection.
- Journal
-
Journal of Controlled Release
2020 Apr 15;S0168-3659(20)30237-6.
Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy
2020
in vivo System
- Title
- Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy
- Description
- Gold nanoparticles (AuNPs) layered with siRNA were developed for anti-cancer therapy. The in vitro anti-cancer effect confirmed that AuNP showed higher degrees of apoptosis as well as suppression of the oncogene expression in a siRNA dose-dependent manner. The in vivo study revealed that mice treated with c-Myc siRNA-incorporated AuNPs showed dramatically decreased tumor size. The researcher observed the biodistribution of c-Myc siRNA-incorporated AuNPs in the body of mice by fluorescence in vivo imaging with Visque InVivo Smart.
- Journal
-
Pharmaceutics
2020 Mar; 12(3): 261.
Multifunctional Dendrimer-Entrapped Gold Nanoparticles for Labeling and Tracking T Cells Via Dual-Modal Computed Tomography and Fluorescence Imaging
2020
in vivo System
- Title
- Multifunctional Dendrimer-Entrapped Gold Nanoparticles for Labeling and Tracking T Cells Via Dual-Modal Computed Tomography and Fluorescence Imaging
- Description
- Poly(amidoamine) (PAMAM) dendrimer-entrapped gold nanoparticles (Au DENPs) conjugated with Fluo-4 was developed as an efficient nanoprobe for monitoring and tracking T cells. The researchers identified T cell labeling efficiency of this nanoprobe by taking fluorescence images of mice in vivo with Visque InVivo Smart after subcutaneous injection of T cells.
- Journal
-
Biomacromolecules
2020 Apr 13;21(4):1587-1595.
Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design
2019
in vivo System
- Title
- Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design
- Description
- Aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1)-derived peptide (AdP) has been developed as a cosmeceutical ingredient for skin anti-aging given its fibroblast-activating (FA) and melanocyte-inhibiting (MI) functions. However, a suitable strategy for the topical delivery of AdP was required due to its low-permeable properties. In this paper, the researcher developed hydrosol-based topical delivery system for AdP fragments which have effects on fibroblast and melanocyte. The optimized hydrosol showed 127-fold higher peptide deposition in STM than free FA-AdP. The quantification of the fluorescent dye-tagged FA-AdP in Strat-M membrane (STM) was carried out by near-infrared fluorescence imaging with Visque InVivo Smart.
- Journal
- Molecules 2019 May 22;24(10):1967
Smart Microcapsules with Molecular Polarity- and Temperature-Dependent Permeability
2019
in vivo System
- Title
- Smart Microcapsules with Molecular Polarity- and Temperature-Dependent Permeability
- Description
- Microcapsules with molecule-selective permeation are appealing as microreactors, capsule-type sensors, drug and cell carriers, and artificial cells. In this paper, the researcher made water-in-oil-in-water (W/O/W) double-emulsion drops from two monomers and dodecanol with capillary microfluidic devices. The oil shell has selective permeability above melting point of dedecanol, which is drastically lowered below the melting point. The release from the oil shell was tested by in vivo fluorescence imaging with Visque InVivo Smart at specific time points after the subcutaneous injection of the ICG-loaded oil shell into the thigh of mice.
- Journal
- Small (Nano Micro Small) 2019 May;15(21):e1900434
Oral Delivery of Honokiol Microparticles for Nonrapid Eye Movement Sleep
2019
in vivo System
- Title
- Oral Delivery of Honokiol Microparticles for Nonrapid Eye Movement Sleep
- Description
- Honokiol (HNK) is a small-molecule lignin extracted from Magnolia Officinalis, promoting nonrapid eye movement (NREM) sleep. However, the treatment of insomnia using HNK is restricted by its extremely low oral bioavailability. In this paper, the researcher made honokiol microparticle by loading honokiol into poly lactide-glycolide acid microparticles (HNK-MP). HNK-MP showed enhanced oral bioavailability, resulting in increased AUC0-12h and maximum blood concentration (Cmax), finally increased NREM sleep after oral administration in in vivo experiment. The researcher tested the biosafety of HNK-MP in vivo, and confirmed no damage in the gastrointestinal tract. The retention in the gastrointestinal tract was identified by ex vivo fluorescence imaging of the gastrointestinal tract with Visque InVivo Elite, after oral administration of HNK-MP to rats.
- Journal
- Molecular Pharmaceutics 2019 Feb 4;16(2):737-743
Biodegradable Mesoporous Silica Achieved via Carbon Nanodots-Incorporated Framework Swelling for Debris-Mediated Photothermal Synergistic Immunotherapy
2019
in vivo System
- Title
- Biodegradable Mesoporous Silica Achieved via Carbon Nanodots-Incorporated Framework Swelling for Debris-Mediated Photothermal Synergistic Immunotherapy
- Description
- The uniformly incorporating polymer-coated carbon nanodots (CDs) into ordered framework of mesoporous silica nanoparticles (CD@MSNs) was developed both for the photothermal therapy (PTT) and immunotherapy of cancer. The obtained CD@MSN showed enhanced photothermal effect and elevated targeting accumulation in vitro and in vivo. And CD@MSN-mediated PTT also showed immune-mediated inhibition of tumor metastasis by natural killer cells and macrophages. They observed biodistribution of CD@MSN by fluorescence imaging of isolated organs with Visque InVivo Smart at specific time points after injection into the body of mice.
- Journal
-
Nano Letters
2019 Dec 11;19(12):8409-8417.
Osteogenesis and angiogenesis are simultaneously enhanced in BMP2-/VEGF-transfected adipose stem cells through activation of the YAP/TAZ signaling pathway.
2019
in vivo System
- Title
- Osteogenesis and angiogenesis are simultaneously enhanced in BMP2-/VEGF-transfected adipose stem cells through activation of the YAP/TAZ signaling pathway.
- Description
- The osteogenic and angiogenic effect of BMP2-/VEGF-transfected adipose stem cells are simultaneously enhanced in critical-size calvarial defects and long-bone segmental defects in immunosuppressed rats. They identified with Visque InVivo Elite that the implanted stem cells did not migrate out of the implantation site by the 56th day. They suggest the possibility of using gene–cell therapy that can induce rapid angiogenesis and osteogenesis in inhospitable avascular environments.
- Journal
- Biomaterials Science
2019 Nov 1;7(11):4588-4602.
Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance
2019
in vivo System
- Title
- Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance
- Description
- Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes were imaged and quantified with Visque InVivo Smart and its SW, Clevue in ex vivo in this paper. They suggest that targeted delivery of the CRISPRi system against Fabp4 to white adipocytes provides a simple and safe approach to regress and treat obesity and obesity-induced metabolic syndromes.
- Journal
- Genome Research
2019 Sep;29(9):1442-1452.
Conjugation of prostate cancer-specific aptamers to polyethylene glycol-grafted polyethylenimine for enhanced gene delivery to prostate cancer cells
2019
in vivo System
- Title
- Conjugation of prostate cancer-specific aptamers to polyethylene glycol-grafted polyethylenimine for enhanced gene delivery to prostate cancer cells
- Description
- The BioDistribution of gene delivery carrier were imaged and quantified with Visque InVivo Smart and its SW, Clevue in ex vivo in this paper. They suggest that PEI-PEG-Wy5a may be useful for prostate cancer-specific gene delivery.
- Journal
-
Journal of Industrial and Engineering Chemistry
2019 May 25;73:182-191
A nanoscale photothermal agent based on a metal-organic coordination polymer as a drug-loading framework for effective combination therapy
2019
in vivo System
- Title
- A nanoscale photothermal agent based on a metal-organic coordination polymer as a drug-loading framework for effective combination therapy
- Description
- The BioDistribution of photothermal agent were imaged and quantified with Visque InVivo Elite and its SW, Clevue in in vivo and ex vivo in this paper. They confirmed that photothermal agent was accumulated in the tumor tissue.
- Journal
- Acta Biomaterialia
2019 Aug;94:435-446.
pH-Responsible Fluorescent Carbon Nanoparticles for Tumor Selective Theranostics via pH-turn on/off Fluorescence and Photothermal Effect In Vivo and In Vitro
2018
in vivo System
- Title
- pH-Responsible Fluorescent Carbon Nanoparticles for Tumor Selective Theranostics via pH-turn on/off Fluorescence and Photothermal Effect In Vivo and In Vitro
- Description
- The new nanoparticle was developed by comprising a photothermal dye (IR825)-loaded carbonized zwitterionic polymer [FNP-I]. This nanoparticle act as a pH-responsive fluorescence probes to sense intracellular cancer cell and for near infrared (NIR) controllable photothermal therapy (PTT) after activation by reaching the cancer cell environment. In the in vitro and in vivo experiment, the nanoparticle showed good cellular uptake and biocompatibility as potential imaging-guided photothermal therapy nanotool for cancer treatment. In the study, the researchers identified the biodistribution inside the body at specific time points by in vivo imaging of whole mice after the intravenous injection of nanoparticle and confirm that the accumulation of nanoparticle in tumor tissue by ex vivo imaging of the organs after the sacrifice with Visque InVivo Elite.
- Journal
- Nanoscale 2018 Feb 1;10(5):2512-2523
Age-related changes in pial arterial structure and blood flow in mice
2016
in vivo System
- Title
- Age-related changes in pial arterial structure and blood flow in mice
- Description
- Age-related changes in cerebral blood flow and vasculature of mice were confirmed by investigating with real time fluorescent imaging ability and feature map analysis abilities of CleVue, the SW of Vieworks.
- Journal
- Neurobiology of Aging.
2016 Jan;37:161-170
Exendin-4 protects hindlimb ischemic injury by inducing angiogenesis
2015
in vivo System
- Title
- Exendin-4 protects hindlimb ischemic injury by inducing angiogenesis
- Description
- The angiogenic effect of exendin-4 using a mouse hindlimb ischemia model was confirmed by analyzing the blood flow in ischemic tissue with real time fluorescent imaging ability and feature map analysis abilities of Clevue, the SW of Vieworks.
- Journal
- Biochemical and Biophysical Research Communications.
2015 Oct 2;465(4):758-63.
Use of indocyanine green for optical analysis of cortical infarcts in photothrombotic ischemic brains
2015
in vivo System
- Title
- Use of indocyanine green for optical analysis of cortical infarcts in photothrombotic ischemic brains
- Description
- The dynamic recovery and blood flow after photothrombotic ischemic injury of brain were analyzed and diagnosed with real time imaging ability and FluAngio and DyAngio algorithm which was developed by collaborated research of Kyung Hee University and Vieworks. CleVue enables equivalent analysis as described in this paper.
- Journal
- Journal of Neuroscience Methods.
2015 Jun 15;248:46-50
Optical measurement of mouse strain differences in cerebral blood flow using indocyanine green
2015
in vivo System
- Title
- Optical measurement of mouse strain differences in cerebral blood flow using indocyanine green
- Description
- The difference of cerebral blood flow between two mouse strain was confirmed by analyzing and comparing with real time fluorescent imaging ability and feature map ability which is developed by collaborated research of Kyung Hee University and Vieworks. CleVue enables equivalent analysis as described in this paper.
- Journal
- Journal of Cerebral Blood Flow & Metabolism.
2015 Jun;35(6):912-6
Noninvasive Optical Measurement of Cerebral Blood Flow in Mice Using Molecular Dynamics Analysis of Indocyanine Green
2012
in vivo System
- Title
- Noninvasive Optical Measurement of Cerebral Blood Flow in Mice Using Molecular Dynamics Analysis of Indocyanine Green
- Description
- The various parameter of blood flow was investigated by analyzing the fluorescence dynamics after real time imaging of cerebral blood flow of mice. CleVue enables equivalent analysis as described in this paper.
- Journal
- PLOS ONE.
2012;7(10):e48383.
Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature
2011
in vivo System
- Title
- Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature
- Description
- The difference of vasculature between tumor and normal tissue were studied by analyzing the dynamics of blood flow after realtime fluorescence imaging with ICG. CleVue enables equivalent analysis as described in this paper.
- Journal
- Journal of Biomedical Optics
2011 Apr;16(4):046008.
Efficient differentiation of human pluripotent stem cells into functional CD34 progenitor cells by combined modulation of the MEK/ERK and BMP4 signaling pathways
2010
in vivo System
- Title
- The vasculogenic effects of stem cell therapy on ischemic tissue of mice were investigated by analyzing the dynamics of blood flow after realtime fluorescence imaging of ischemic tissue. CleVue enables equivalent analysis as described in this paper.
- Description
- The vasculogenic effects of stem cell therapy on ischemic tissue of mice were investigated by analyzing the blood flow of ischemic tissue.
- Journal
- BLOOD.
2010 Dec16;116(25):5762-72
Unsorted human adipose tissue-derived stem cells promote angiogenesis and myogenesis in murine ischemic hindlimb model
2010
in vivo System
- Title
- Unsorted human adipose tissue-derived stem cells promote angiogenesis and myogenesis in murine ischemic hindlimb model
- Description
- The promotion effect on angiogenesis and myogenesis in mouse ischemic hindlimb model after treating stem cells is identified by analyzing the dynamics of blood flow after realtime fluorescence imagin in ischemic tissue. CleVue enables equivalent analysis as described in this paper.
- Journal
- Microvascular Research.
2010 Dec;80(3):310-6.
Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics
2009
in vivo System
- Title
- Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics
- Description
- The relation between perfusion rate and necrosis was investigated by analyzing the blood flow after realtime fluorescence imaging in mouse ischemic hindlimb model. CleVue enables equivalent analysis as described in this paper.
- Journal
- PLOS One. 2009;4(1):e4275.
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