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Title Year
Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer 2021
Title
Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer
Description
Trastuzumab is the main component of HER2-directed gastric cancer therapy, but patient response is poor due to insufficient cell sensitivity and drug resistance. In this paper, the researchers report that HER2 is involved in cell mitotic promotion for tumorigenesis and a poor response of patients to trastuzumab therapy by hyperactivating a crucial HER2-SHCBP1-PLK1 axis. Theaflavine-3, 3’-digallate (TFBG), an inhibitor of the SHCBP1-PLK1 interaction, acts as a potential trastuzumab sensitizer and shows high efficacy in inhibiting the growth of HER2-positive gastric cancer when combined with trastuzumab. The longitudinal response of NCI-N87 tumor to trastuzumab is observed by in vivo luminescence imaging with Visque InVivo Smart-LF after the treatment of trastuzumab with knockdown of SHCBP1 or TFBG treatment on NCI-N87/luc tumor xenograft model mice.
Journal
Nature Communications 2021 May 14;12(1):2812
Lung-selective 25-hydroxycholesterol nanotherapeutics as a suppressor of COVID-19-associated cytokine storm 2021
Title
Lung-selective 25-hydroxycholesterol nanotherapeutics as a suppressor of COVID-19-associated cytokine storm
Description
Decreased 25-hydroxycholesterol (25-HC) and increased cytokine levels is believed to play a crucial role in COVID-19 pathogenesis, inducing acute respiratory distress syndrome (ARDS) and sepsis. In this paper, the researchers develop 25-HC and didodecyldimethylammonium bromide (DDAB) nanovesicles (25-HC@DDAB) as a COVID-19 drug candidate for the restoration of intracellular cholesterol level and suppression of cytokine storm in severe SARS-CoV-2 patients. 25-HC@DDAB can selectively accumulate the lung tissues and effectively reduce inflammatory cytokine levels in COVID-19 patient-derived PBMCs. The researchers confirm the accumulation of 25-HC@DDAB in lung by ex vivo fluorescence imaging with Visque InVivo Smart at the specific time point after intravenous injection of 25-HC@DDAB into the tail vein of mice.
Journal
Nano Today 2021 Jun; 38: 101149 2021 Jan 11;
RNA interference-mediated suppression of TNF-α converting enzyme as an alternative anti-TNF-α therapy for rheumatoid arthritis 2021
Title
RNA interference-mediated suppression of TNF-α converting enzyme as an alternative anti-TNF-α therapy for rheumatoid arthritis
Description
For approximately 90% of patients with RA, anti-TNF-α therapy is treated as the first-line immuno-treatment since excessive tumor necrosis factor-α (TNF-α) is associated with the pathogenesis of rheumatoid arthritis (RA). In this paper, researchers suggested suppression of TNF-α converting enzyme (TACE) as a novel strategy to down-regulate TNF-α level. They developed a disease site-targeted RNA interference system by forming non-viral complex between shRNA against TACE (shTACE) and bone resorption site-specific peptide carrier. In collagen induced arthritis (CIA) model experiments, the shTACE/peptide carrier complex alleviated arthritic symptoms with enhanced anti-inflammatory and anti-osteoclastogenic effects. And, similar results were obtained with human origin cell experiments from tissues and synovial fluids of RA patients. The biodistribution of shTACE/peptide carrier complex was identified by in vivo fluorescence imaging with Visque InVivo Smart after intravenous injection of shTACE/peptide carrier complex into CIA model mice.
Journal
Journal of Controlled Release 2021 Feb 10;330:1300-1312
Fenton-like reaction, glutathione reduction, and photothermal ablation-built-in hydrogels crosslinked by cupric sulfate for loco-regional cancer therapy 2021
Title
Fenton-like reaction, glutathione reduction, and photothermal ablation-built-in hydrogels crosslinked by cupric sulfate for loco-regional cancer therapy
Description
In this paper, the researchers developed HC/Cu/ICG hydrogel using crosslinked hydrogel systems to apply Fenton-like reaction-associated chemodynamic therapy (CDT) and hyperthermia-inducing photothermal therapy (PTT) for loco-regional cancer therapy. HC/Cu/ICG hydrogel was developed by incorporating Cupric sulfate (Cu), catechol-functionalized hyaluronic acid (HC) and indocyanine green (ICG). HC/Cu/ICG hydrogel showed therapeutic efficiency in the loco-regional CDT and PTT of cancers while not inducing any severe systemic toxicities in in vivo experiments. The Biodegradability of HC/Cu/ICG hydrogel was investigated by in vivo fluorescence imaging with Visque InVivo Smart at the specific days after the subcutaneous injection of HC/Cu/ICG hydrogel into mice.
Journal
Biomaterials Science 2021 Feb 7;9(3):847-860
Dissecting the impact of target-binding kinetics of protein binders on tumor localization 2021
Title
Dissecting the impact of target-binding kinetics of protein binders on tumor localization
Description
To improve the therapeutic effects of protein-based therapeutics, their in vivo behavior is considered and controlled systematically. In this paper, the researchers investigate the influence of the binding affinity of proteins on tumor localization by using four repebodies having different affinities to EGFR on cancer model mice. They find that optimal affinity with balanced target binding and dissociation can facilitate deep penetration and accumulation of protein binders in tumors by overcoming the binding-site-barrier effect. The BioDistribution of repebodies in the body is identified and quantitated by in vivo fluorescence imaging with Visque InVivo Smart-LF at specific time points after the intravenous injection of various repebody into A431 tumor xenograft model mice.
Journal
iScience 2021 Feb 19; 24(2): 102104.
Antibreast Cancer Activity of Aspirin-Conjugated Chalcone Polymeric Micelles 2021
Title
Antibreast Cancer Activity of Aspirin-Conjugated Chalcone Polymeric Micelles
Description
In this paper, the researchers developed new aspirin-conjugated chalcone derivative-loaded nanoparticle (AS-DK143-loaded NP) using mPEG-PLA co-polymer. AS-DK143 NP showed the reduction of cell viability of 4T1 cells in vitro and anti-cancer efficacy with the selective targetability on cancer and the reduction of tumor size in 4T1-luc cell bearing mice. The BioDistribution of AS-DK143 NP in the body is identified by ex vivo fluorescence imaging of the extracted tissues with Visque InVivo Smart at specific time points after the subcutaneous injection into 4T1 xenograft mice.
Journal
Macromolecular Research 2021 Jan 28;29:105–110
Meso-pyridinium substituted BODIPY dyes as mitochondria-targeted probes for the detection of cysteine in living cells and in vivo 2021
Title
Meso-pyridinium substituted BODIPY dyes as mitochondria-targeted probes for the detection of cysteine in living cells and in vivo
Description
Cysteine (Cys) is a bio-thiol acting as an antioxidant against ROS which can cause oxidative damage in mitochondria. The researchers develop BDP-S-o-Py+ as fluorescent probes by substition of and meso-pyridinium of BODIPY, which is a mitochondria-targeted probe for Cys-specific detection. BDP-S-o-Py+ can detect mitochondrial Cys in living cells in vito, and endogenous Cys in vivo. BDP-S-o-Py+ showed satisfactory capability to image Cys in vivo in in vivo fluorescence imaging with Visque InVivo Smart after the subcutaneous injection of BDP-S-o-Py+ and Cys into mouse.
Journal
Dyes and Pigments 2021 Mar;187:109089
Characterization of adipose-derived stromal/stem cell spheroids versus single-cell suspension in cell survival and arrest of osteoarthritis progression 2021
Title
Characterization of adipose-derived stromal/stem cell spheroids versus single-cell suspension in cell survival and arrest of osteoarthritis progression
Description
In this paper, researchers tested spheroidal adipose-derived stromal/stem cells (ASCs) for the treat of surgically induced osteoarthritis (OA) in a rat model. ASC spheroids cultured in 3D have better in vitro and in vivo cell survival and chondrogenic potential, resulting in better regeneration and the arrest of surgically induced OA than ASCs in single-cell suspension. ASCs in the knee joints of rats with surgically induced OA were tracked by fluorescence in vivo imaging with Visque InVivo Smart at the specific time point after the intra-articular injection of ASCs.
Journal
Journal of Biomedical Materials Research Part A 2021 Jun;109(6):869-878
Optically activatable photosynthetic bacteria-based highly tumor specific immunotheranostics 2020
Title
Optically activatable photosynthetic bacteria-based highly tumor specific immunotheranostics
Description
In this paper, the non-pathogenic natural purple photosynthetic bacteria (PPSB) is investigated as bacteria for bacterial therapy in solid tumor treatment. PPSB exhibit highly targeted tumor elimination and precisely marking tumor location by showing strong NIR-I-to-NIR-II reporter fluorescence, powerful photothermal conversion, excellent reactive oxygen species generation, and contrasting photo-acoustic effects. The optical efficacies of the bacterial treatment is dramatically improved with short-term fasting. The tumor targetability of PPSB is investigated with the biodistribution of PPSB by in vivo fluorescence imaging and ex vivo fluorescence imaging with Visque InVivo Smart-LF at specific time points after intravenous injection of PPSB into Colon26 tumor-bearing mice.
Journal
Nano Today 2020 Apr;37:101100
Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta–Mediated Epithelial–Mesenchymal Transition 2020
Title
Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta–Mediated Epithelial–Mesenchymal Transition
Description
Protein transduction domain (PTD) fused BMP-7 in micelle (mPTD-BMP-7) is developed as the effective therapeutics for renal fibrosis. mPTD-BMP-7 successfully inhibits the TGF-β–mediated epithelial–mesenchymal transition process in vitro and in an in vivo unilateral ureter obstruction model. The researchers identified the clinical relevance of mPTD-BMP-7 by developing and applying an intra-arterial administration of mPTD-BMP-7 through renal artery in pigs. mPTD-BMP-7 through renal artery intervention effectively delivered into Bowman’s space and inhibits unilateral ureter obstruction–induced renal fibrosis in pigs. The biodistribution of mPTD-BMP-7 in the kidney was observed using ex vivo fluorescence imaging of the kidney with Visque InVivo Smart at specific time points after the intra-arterial administration of mPTD-BMP-7 through renal artery.
Journal
Frontiers in Pharmacology 2020 Nov 19;11:591275
Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer 2020
Title
Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer
Description
In this paper, researchers developed a biophotonic nanoplatform by integrating the zirconium carbide (ZrC) nanosheet as a deep PTT-photosensitizer and on-demand designed anticancer prodrug SN38-Nif. The integrated ZrC@prodrug biophotonic nanoplatform shows the potent anticancer effects in in vitro and in vivo experiments. In mouse models, the ZrC@prodrug system markedly inhibits tumor recurrence, metastasis, inflammation and angiogenesis. The synergistic inhibition of tumor growth with ZrC@prodrug was confirmed by ex vivo fluorescence imaging of tumors in liver with Visque InVivo Elite at a specific time point after the treatment on mouse model.
Journal
Advanced Science 2020 Nov 5;7(24):2001191
Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis 2020
Title
Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis
Description
The bone morphogenetic protein-7 (BMP-7) is a therapeutic growth factor reverting many fibrotic diseases, including peritoneal fibrosis by peritoneal dialysis (PD). The researcher developed a novel drug delivery system using protein transduction domains (PTD) and made PTD-BMP-7 by applying it on BMP-7. In in vivo study using rats, PTD-BMP-7 showed the therapeutic effects on epithelial-mesenchymal transition (EMT)-related fibrosis by restoring the changes of peritoneum and suppress the progress of established PD fibrosis. The researchers investigated the biodistribution and the half-life of PTD-BMP-7 by fluorescence in vivo imaging with Visque InVivo Elite and fluorescence ex vivo imaging with Visque InVivo Smart at the specific time point after the intra-peritoneal injection of PTD-BMP-7 into mice.
Journal
Journal of Cellular and Molecular Medicine 2020 Nov;24(22):13507-13522
“Star” miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer 2020
Title
“Star” miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer
Description
In this paper, DPC/miR-34a nanoplex was developed by self-assembly of Dextrin modified 1.8 k PEI with CM-end (Dextrin-PEI-CM, DPC) and Star miR-34a, a cell adhesion molecules CD44 inhibitor. DPC/miR-34a nanoplex showed good ability in suppression of cancer cell invasion in an in vitro cell invasion assay and in vivo anti-metastasis model. And, DPC/miR-34a demonstrated a superior antitumor and anti-metastatic efficacy both in lung metastatic model and orthotopic MDA-MB-231 tumor models. The biodistribution of DPC/miR-34a was identified by fluorescence in vivo and ex vivo imaging with Visque InVivo Elite at the specific time points after the injection of DPC/miR-34a into the tail vein of MDA-MB-231 tumor model mice.
Journal
Journal of Controlled Release 2020 Oct 10;326:615-627
Ferritin Nanocage-Based Methyltransferase SETD6 for COVID-19 Therapy 2020
Title
Ferritin Nanocage-Based Methyltransferase SETD6 for COVID-19 Therapy
Description
In this paper, the ferritin nanocage-based peptide delivery system was used for the delivery of cell-penetrating peptide and nuclear-localizing TAT-NBD peptide with SETD6. TAT NBD-short ferritin-SETD6 (TFS) enhances the SETD6 level and reduces the nuclear factor-κB (NF-κB) signaling. The TFS make the survival rate of cytokine storm model mouse better and the lung tissue damage and cytokine expression reduced. The biodistribution of TFS in cytokine storm model mouse was observed by fluorescence ex vivo imaging of organs with Visque InVivo Smart at the specific time point after the i.v. injection of TFS into mouse body.
Journal
Advanced Functional Materials 2020 Nov 25;30(48):2006110
A brain tumor-homing tetra-peptide delivers a nano-therapeutic for more effective treatment of a mouse model of glioblastoma 2020
Title
A brain tumor-homing tetra-peptide delivers a nano-therapeutic for more effective treatment of a mouse model of glioblastoma
Description
Organ-specific cell-penetrating peptides (CPPs) are a kind of molecules that can deliver drugs very effectively, and they are currently of great interest in cancer treatment strategies. In this paper, the researchers developed amino acid sequence serine-isoleucine-tyrosine-valine(SIWV) as a new CPPs to target glioblastoma multiforme (GBM) brain tumor tissues. In in vitro and in vivo experiments, enhanced in vivo targeting ability of SIWV was showed. The biodistribution and capability as a targeting agents were confirmed by fluorescence in vivo imaging and ex vivo imaging with Visque InVivo Smart-LF at specific time points after the injection of SIWV linked with SN-38, the anticancer drug, into the body of GBM xenograft mice.
Journal
Nanoscale Horizons 2020 Jul 27;5(8):1213-1225
Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature 2020
Title
Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature
Description
In this paper, the researchers develop 2 jet-lagged nanoparticles, apatinib (APA)-loaded TEC-targeting nanodrug (APA/MCP) for vascular normalization therapy and lonidamine (LND)-loaded tumor cell-targeting nanodrug (LND/MCA) for tumor cell metabolic treatment. APA/MCP can block VEGF/VEGFR2 to inhibit tumor endothelial cells (TECs) proliferation and LND/MCA can inhibit lactic acid (LA) efflux to remodel tumor acid metabolism. Moreover, the results of in vivo pharmacodynamic studies show that the therapeutic effect of programmed death 1 (PD-1) drug with jet-lagged nanoparticles is improved 3-folds to that of the PD-1 group. Tumor targetability and distribution behavior of Jet-lagged nanoparticles were identified by fluorescence in vivo imaging and ex vivo imaging with Visque InVivo Elite at specific time points after the injection of Jet-lagged nanoparticles into B16F10 bearing mice.
Journal
Advanced Healthcare Materials 2020 Jun;9(12):e2000075
Dual functional nanoparticles containing SOX duo and ANGPT4 shRNA for osteoarthritis treatment 2020
Title
Dual functional nanoparticles containing SOX duo and ANGPT4 shRNA for osteoarthritis treatment
Description
In this study, the researchers designed nanoparticles comprising dexamethasone-conjugated polyethylenimine (DEXPEI) complexed with minicircle plasmid (MC) harboring SOX duo (SOX-9, -6) and ANGPTL4 small hairpin RNA (shANG) [MCSOX9/6/shANG] to enhance chondrogenesis of stem cells and suppress inflammation in OA. Adipose-derived stem cells (ADSCs) transfected with MCSOX9/6/shANG (MCSOX9/6/shANG-tADSCs) showed enhanced chondrogenesis and suppressed progression of osteoarthritis (OA) in both in vitro and in vivo experiments. Fluorescence labeled MCSOX9/6/shANG-tADSCs were tracked by in vivo fluorescence imaging with Visque InVivo Elite at specific time points after injection of MCSOX9/6/shANG-tADSCs into right knee joint of rats in which OA had been surgically induced.
Journal
Journal of Biomedical Materials Research Part B: Applied Biomaterials 2020 Jan;108(1):234-242
Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery 2020
Title
Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery
Description
In this paper, the researchers suggest a noninvasive active-targeting therapy for ischemic stroke using a neutrophil-like cell-membrane-coated mesoporous Prussian blue nanozyme (MPBzyme@NCM). Despite its robust anti-inflammatory and antioxidative stress properties, nanozyme has shown unsatisfactory therapeutic effects due to its insufficient accumulation in the ischemic brain via noninvasive administration. The researchers improve its delivery to the lesion site by coating the nanozyme with the neutrophil-like membrane. They also demonstrate the long-term in vivo therapeutic efficacy for ischemic stroke after the delivery into the damaged brain. The in vivo fluorescence imaging with Visque InVivo Elite is used to confirm the accumulation of MPBzyme@NCM at the lesion site over time.
Journal
ACS Nano
Interrogation of Folic Acid-Functionalized Nanomedicines: The Regulatory Roles of Plasma Proteins Reexamined 2020
Title
Interrogation of Folic Acid-Functionalized Nanomedicines: The Regulatory Roles of Plasma Proteins Reexamined
Description
Folic acid (FA) has been widely used to promote targeted delivery of nanomedicines to tumors since the folate receptor-α (FR-α) is overexpressed in many human cancers. In this paper, the researchers find that the FA functionalization induces heavy natural IgM absorption on the liposomal surface, preventing FA from binding receptors and accelerating complement activation in vivo. In addition, FA-functionalized polymeric nanoparticles are found to be vulnerable to natural IgM absorption. The biodistribution of FA-functionalized liposome (FA-sLip) at specific time points after the tail vein is observed using in vivo fluorescence imaging with Visque InVivo Elite.
Journal
ACS Nano
Supramolecular Container-Mediated Surface Engineering Approach for Regulating the Biological Targeting Effect of Nanoparticles 2020
Title
Supramolecular Container-Mediated Surface Engineering Approach for Regulating the Biological Targeting Effect of Nanoparticles
Description
In this paper, researchers suggest a supramolecular container-based surface engineering approach to make a chemical bridge to incorporate nanoparticles and targeting ligands. The researchers use an acyclic cucurbituril (aCB) molecular container as a supramolecular container to incorporate nanoparticles and targeting ligands via a bilateral host-guest complexation, enabling better water dispersibility and enhanced biological targeting. The aCB-engineered nanoparticles show the enhanced biological targeting effect and targeted imaging performance in in vitro and in vivo experiments. The enhanced targeting and imaging performance are demonstrated by the fluorescence imaging of tumor-bearing mice with Visque InVivo Elite at the specific time point after intravenous injection of rhodamine B isothiocyanate (RITC)-labeled nanoparticle.
Journal
Nano Letters
Enhancement of Nanozyme Permeation by Endovascular Interventional Treatment to Prevent Vascular Restenosis via Macrophage Polarization Modulation 2020
Title
Enhancement of Nanozyme Permeation by Endovascular Interventional Treatment to Prevent Vascular Restenosis via Macrophage Polarization Modulation
Description
In this paper, the researchers examine nanozymes as an effective therapeutic strategy for vascular restenosis after endovascular interventional treatment. Nanozymes are excellent at scavenging ROS, the major aggravating factor for vascular restenosis, but cannot efficiently pass through the vascular endothelial cell barrier to reach the vascular intima. The researchers discover that the endothelial cells exfoliation by endovascular interventional treatment enhances nanoparticle permeation into the vascular intima and uptake by macrophages to alleviate long-term vascular restenosis in vivo. The vascular restenosis is relieved after vascular balloon injury (VBI) under the presence of Prussian blue nanozyme (PBzyme). The accumulation of PBzyme in left common carotid arteries of mice is confirmed with in vivo fluorescence imaging using Visque InVivo Elite.
Journal
Advanced Functional Materials
A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues 2020
Title
A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues
Description
In this paper, the researchers develop a specific substrate for Acetylcholinesterase (AChE) detection by combining dimethylcarbamate choline with a self-immolative scaffold. Imaging the activity of AChE which is tightly associated with neurological diseases is difficult due to the interference from butyrylcholinesterase (BChE) and carboxylesterase (CE). The representative substrate P10 can effectively eliminate the interference from CE and BChE. Its high specificity is confirmed via imaging AChE activity in vitro. Also, the AChE activity in a normal mouse brain is successfully captured via in vivo and ex vivo fluorescence imaging with Visque InVivo Elite.
Journal
Chemical Science
Molecular Design of Conjugated Small Molecule Nanoparticles for Synergistically Enhanced PTT/PDT 2020
Title
Molecular Design of Conjugated Small Molecule Nanoparticles for Synergistically Enhanced PTT/PDT
Description
In this paper, IID-ThTPA nanoparticles (IID-ThTPA NPs) are proposed as a new phototheranostic agent for simultaneous photothermal therapy (PTT) and photodynamic therapy (PDT). The researchers design a donor–acceptor–donor (D–A–D) conjugated small molecule (IID-ThTPA)-based phototheranostic agent, with isoindigo (IID) as the selective acceptor and triphenylamine (TPA) as the donor. This IID-ThTPA shows superior tumor cooperative eradicating capability in vitro and in vivo with a high PTT/PDT performance. The BioDistribution of IID-ThTPA nanoparticles in the body is identified by ex vivo fluorescence imaging of the extracted tissues with Visque InVivo Elite at specific time points after the intravenous injection.
Journal
Nano-Micro Letters
In Vivo Imaging of Senescent Vascular Cells in Atherosclerotic Mice Using a β-Galactosidase-Activatable Nanoprobe 2020
Title
In Vivo Imaging of Senescent Vascular Cells in Atherosclerotic Mice Using a β-Galactosidase-Activatable Nanopro
Description
Detection of senescence-associated β-galactosidase (SA-β-Gal) is suggested as the early diagnosis and therapy of atherosclerosis and other aging-associated diseases. The researchers develop a β-galactosidase-activatable nanoprobe BOD-L-βGal-NPs to detect SA-β-Gal which is overexpressed in senescent cells. Nanoprobe BOD-L-βGal-NPs showed good accumulation in arteries, thus successfully visualizing senescent cells and vasculature in atherosclerotic mice by in vivo fluorescence imaging after tail vein injection. The difference between the control group and the aging group is identified by in vivo fluorescence imaging with Visque InVivo Smart after tail vein injection of BOD-L-βGal-NPs into mice. And the accumulation of BOD-L-βGal-NPs in arteries is confirmed by ex vivo fluorescence imaging with Visque InVivo Smart.
Journal
Analytical Chemistry
Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy 2020
Title
Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy
Description
Monoiodo Aza-BODIPY is developed as a new Near-Infrared Photosensitizer (PS) for Photodynamic therapy (PDT), which is a rising platform of the cancer treatment method, and its antitumor efficacy is demonstrated both in vivo and in vitro. BODIPYs possess many advantages of ideal PS characteristics and aza-BODIPYs among them have large absorption coefficient and NIR absorption to fit the therapeutic window (650 - 900nm). The researchers make many substitution variations on aza-BODIPY and find that monoiodo derivatives of aza-BODIPY have various advantages of an ideal PS with excellent photostability, chemical stability, and photocytotoxicity. The superior antitumor efficacy of Monoiodo Aza-BODIPY is demonstrated both in vitro and in vivo. The biodistribution of Monoiodo Aza-BODIPY at specific time points after the tail vein injection is explored using in vivo and ex vivo fluorescence imaging of mice with Visque InVivo Smart.
Journal
Journal of Medicinal Chemistry
2020 Sep 10;63(17):9950-9964
Detecting Cysteine in Bioimaging with a Near‐Infrared Probe Based on a Novel Fluorescence Quenching Mechanism 2020
Title
Detecting Cysteine in Bioimaging with a Near‐Infrared Probe Based on a Novel Fluorescence Quenching Mechanism
Description
The highly selective and sensitive NIR turn-on fluorescent probe (BDP-NIR) was developed to detect cysteine (Cys) in biological systems. BDP-NIR was based on BODIPY with a large Stokes shift (105 nm) when detecting Cys. BDP-NIR had a high linear dynamic range from 0 μM to 500 μM, which was promising for detecting of Cys quantificationally. The researchers took fluorescence imaging of mice with Visque InVivo Elite to evaluate the capability of sensing endogenous Cys in vivo.
Journal
ChemBioChem
Heme Oxygenase 1-Targeted Hybrid Nanoparticle for Chemo- and Immuno-Combination Therapy in Acute Myelogenous Leukemia 2020
Title
Heme Oxygenase 1-Targeted Hybrid Nanoparticle for Chemo- and Immuno-Combination Therapy in Acute Myelogenous Leukemia
Description
The new hybrid nanoparticle is developed as a novel therapeutic in acute myelogenous leukemia (AML) and its potential was assessed with a human AML-bearing orthotopic mouse model. The lipid-polymer hybrid nanoparticle (hNP) is loaded with tin mesoporphyrin (SnMP), an inhibitor of Heme Oxygenase 1 (HO1) which is an antioxidative and cytoprotective enzyme inducing chemo-resistant AML, and an engineered antibody for leukemic cell-targeted delivery. HO1-inhibiting T-hNP (T-hNP/SnMP) enhances chemo-sensitivity in human leukemia cells. The T-hNP/SnMP enhances the chemo-therapeutic effect of daunorubicin and boosts immune response by reprogramming bone marrow myeloid cells. In the ex vivo study, the researchers identify the organ distribution of T-hNP/SnMP at specific time points after the intravenous injection and confirm the presence of T-hNP/SnMP in the bone marrow by fluorescence imaging of mice with Visque InVivo Smart.
Journal
Advanced Science
2020 Jul; 7(13): 2000487
Penta-fluorophenol: A Smiles rearrangement-inspired cysteine-selective fluorescent probe for imaging of human glioblastoma 2020
Title
Penta-fluorophenol: A Smiles rearrangement-inspired cysteine-selective fluorescent probe for imaging of human glioblastoma
Description
The electron-deficient penta-fluorophenol (NPO-B) was developed as a sensing fluorescence probe to measure the cysteine (Cys) level, which is a biomarker of glioblastomoa (GBM). The probe provided a significant fluorescence response with high selectivity, high sensitivity, a fast response time. The researcher identified that this probe can be used for a relatively simple diagnosis and the tracking of GBM at clinical sites by ex vivo fluorescence imaging in both mouse models and human tissue samples with Visque InVivo Elite.
Journal
Chemical Science
2020 May 11; 5658-5668
Selenium and dopamine-crosslinked hyaluronic acid hydrogel for chemophotothermal cancer therapy 2020
Title
Selenium and dopamine-crosslinked hyaluronic acid hydrogel for chemophotothermal cancer therapy
Description
Sodium selenite (Se)-directed crosslinked hydrogels based on hyaluronic acid (HA)-dopamine (HD), including indocyanine green (ICG), were developed for local cancer therapy. This HD/Se/ICG gel showed easy injectability, slow biodegradability and sufficient photothermal efficiencies with NIR laser irradiation, providing efficient tumor growth inhibition capability without severe systemic toxicities. The biodegradability of the gel in the body was tested by in vivo fluorescence imaging of mice with Visque InVivo Smart at specific time points after the intratumoral injection.
Journal
Journal of Controlled Release
2020 Apr 15;S0168-3659(20)30237-6.
Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy 2020
Title
Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy
Description
Gold nanoparticles (AuNPs) layered with siRNA were developed for anti-cancer therapy. The in vitro anti-cancer effect confirmed that AuNP showed higher degrees of apoptosis as well as suppression of the oncogene expression in a siRNA dose-dependent manner. The in vivo study revealed that mice treated with c-Myc siRNA-incorporated AuNPs showed dramatically decreased tumor size. The researcher observed the biodistribution of c-Myc siRNA-incorporated AuNPs in the body of mice by fluorescence in vivo imaging with Visque InVivo Smart.
Journal
Biomacromolecules
2020 Mar; 12(3): 261.
Multifunctional Dendrimer-Entrapped Gold Nanoparticles for Labeling and Tracking T Cells Via Dual-Modal Computed Tomography and Fluorescence Imaging 2020
Title
Multifunctional Dendrimer-Entrapped Gold Nanoparticles for Labeling and Tracking T Cells Via Dual-Modal Computed Tomography and Fluorescence Imaging
Description
Poly(amidoamine) (PAMAM) dendrimer-entrapped gold nanoparticles (Au DENPs) conjugated with Fluo-4 was developed as an efficient nanoprobe for monitoring and tracking T cells. The researchers identified T cell labeling efficiency of this nanoprobe by taking fluorescence images of mice in vivo with Visque InVivo Smart after subcutaneous injection of T cells.
Journal
Biomacromolecules
2020 Apr 13;21(4):1587-1595.
Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design 2019
Title
Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design
Description
Aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1)-derived peptide (AdP) has been developed as a cosmeceutical ingredient for skin anti-aging given its fibroblast-activating (FA) and melanocyte-inhibiting (MI) functions. However, a suitable strategy for the topical delivery of AdP was required due to its low-permeable properties. In this paper, the researcher developed hydrosol-based topical delivery system for AdP fragments which have effects on fibroblast and melanocyte. The optimized hydrosol showed 127-fold higher peptide deposition in STM than free FA-AdP. The quantification of the fluorescent dye-tagged FA-AdP in Strat-M membrane (STM) was carried out by near-infrared fluorescence imaging with Visque InVivo Smart.
Journal
Molecules 2019 May 22;24(10):1967
Smart Microcapsules with Molecular Polarity- and Temperature-Dependent Permeability 2019
Title
Smart Microcapsules with Molecular Polarity- and Temperature-Dependent Permeability
Description
Microcapsules with molecule-selective permeation are appealing as microreactors, capsule-type sensors, drug and cell carriers, and artificial cells. In this paper, the researcher made water-in-oil-in-water (W/O/W) double-emulsion drops from two monomers and dodecanol with capillary microfluidic devices. The oil shell has selective permeability above melting point of dedecanol, which is drastically lowered below the melting point. The release from the oil shell was tested by in vivo fluorescence imaging with Visque InVivo Smart at specific time points after the subcutaneous injection of the ICG-loaded oil shell into the thigh of mice.
Journal
Small (Nano Micro Small) 2019 May;15(21):e1900434
Oral Delivery of Honokiol Microparticles for Nonrapid Eye Movement Sleep 2019
Title
Oral Delivery of Honokiol Microparticles for Nonrapid Eye Movement Sleep
Description
Honokiol (HNK) is a small-molecule lignin extracted from Magnolia Officinalis, promoting nonrapid eye movement (NREM) sleep. However, the treatment of insomnia using HNK is restricted by its extremely low oral bioavailability. In this paper, the researcher made honokiol microparticle by loading honokiol into poly lactide-glycolide acid microparticles (HNK-MP). HNK-MP showed enhanced oral bioavailability, resulting in increased AUC0-12h and maximum blood concentration (Cmax), finally increased NREM sleep after oral administration in in vivo experiment. The researcher tested the biosafety of HNK-MP in vivo, and confirmed no damage in the gastrointestinal tract. The retention in the gastrointestinal tract was identified by ex vivo fluorescence imaging of the gastrointestinal tract with Visque InVivo Elite, after oral administration of HNK-MP to rats.
Journal
Molecular Pharmaceutics 2019 Feb 4;16(2):737-743
pH-Responsible Fluorescent Carbon Nanoparticles for Tumor Selective Theranostics via pH-turn on/off Fluorescence and Photothermal Effect In Vivo and In Vitro 2018
Title
pH-Responsible Fluorescent Carbon Nanoparticles for Tumor Selective Theranostics via pH-turn on/off Fluorescence and Photothermal Effect In Vivo and In Vitro
Description
The new nanoparticle was developed by comprising a photothermal dye (IR825)-loaded carbonized zwitterionic polymer [FNP-I]. This nanoparticle act as a pH-responsive fluorescence probes to sense intracellular cancer cell and for near infrared (NIR) controllable photothermal therapy (PTT) after activation by reaching the cancer cell environment. In the in vitro and in vivo experiment, the nanoparticle showed good cellular uptake and biocompatibility as potential imaging-guided photothermal therapy nanotool for cancer treatment. In the study, the researchers identified the biodistribution inside the body at specific time points by in vivo imaging of whole mice after the intravenous injection of nanoparticle and confirm that the accumulation of nanoparticle in tumor tissue by ex vivo imaging of the organs after the sacrifice with Visque InVivo Elite.
Journal
Nanoscale 2018 Feb 1;10(5):2512-2523
Biodegradable Mesoporous Silica Achieved via Carbon Nanodots-Incorporated Framework Swelling for Debris-Mediated Photothermal Synergistic Immunotherapy 2019
Title
Biodegradable Mesoporous Silica Achieved via Carbon Nanodots-Incorporated Framework Swelling for Debris-Mediated Photothermal Synergistic Immunotherapy
Description
The uniformly incorporating polymer-coated carbon nanodots (CDs) into ordered framework of mesoporous silica nanoparticles (CD@MSNs) was developed both for the photothermal therapy (PTT) and immunotherapy of cancer. The obtained CD@MSN showed enhanced photothermal effect and elevated targeting accumulation in vitro and in vivo. And CD@MSN-mediated PTT also showed immune-mediated inhibition of tumor metastasis by natural killer cells and macrophages. They observed biodistribution of CD@MSN by fluorescence imaging of isolated organs with Visque InVivo Smart at specific time points after injection into the body of mice.
Journal
Nano Letters
2019 Dec 11;19(12):8409-8417.
Osteogenesis and angiogenesis are simultaneously enhanced in BMP2-/VEGF-transfected adipose stem cells through activation of the YAP/TAZ signaling pathway. 2019
Title
Osteogenesis and angiogenesis are simultaneously enhanced in BMP2-/VEGF-transfected adipose stem cells through activation of the YAP/TAZ signaling pathway.
Description
The osteogenic and angiogenic effect of BMP2-/VEGF-transfected adipose stem cells are simultaneously enhanced in critical-size calvarial defects and long-bone segmental defects in immunosuppressed rats.
They identified with Visque InVivo Elite that the implanted stem cells did not migrate out of the implantation site by the 56th day.
They suggest the possibility of using gene–cell therapy that can induce rapid angiogenesis and osteogenesis in inhospitable avascular environments.
Journal
Biomaterials Science.
2019 Nov 1;7(11):4588-4602.
Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance 2019
Title
Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance
Description
Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes were imaged and quantified with Visque InVivo Smart and its SW, Clevue in ex vivo in this paper. They suggest that targeted delivery of the CRISPRi system against Fabp4 to white adipocytes provides a simple and safe approach to regress and treat obesity and obesity-induced metabolic syndromes.
Journal
Genome Research
2019 Sep;29(9):1442-1452.
Conjugation of prostate cancer-specific aptamers to polyethylene glycol-grafted polyethylenimine for enhanced gene delivery to prostate cancer cells 2019
Title
Conjugation of prostate cancer-specific aptamers to polyethylene glycol-grafted polyethylenimine for enhanced gene delivery to prostate cancer cells
Description
The BioDistribution of gene delivery carrier were imaged and quantified with Visque InVivo Smart and its SW, Clevue in ex vivo in this paper. They suggest that PEI-PEG-Wy5a may be useful for prostate cancer-specific gene delivery.
Journal
Journal of Industrial and Engineering Chemistry
2019 May 25;73:182-191.
A nanoscale photothermal agent based on a metal-organic coordination polymer as a drug-loading framework for effective combination therapy 2019
Title
A nanoscale photothermal agent based on a metal-organic coordination polymer as a drug-loading framework for effective combination therapy
Description
The BioDistribution of photothermal agent were imaged and quantified with Visque InVivo Elite and its SW, Clevue in this paper.
Journal
Acta Biomaterialia
2019 Aug;94:435-446.
pH-Responsible Fluorescent Carbon Nanoparticles for Tumor Selective Theranostics via pH-turn on/off Fluorescence and Photothermal Effect In Vivo and In Vitro 2018
Title
pH-Responsible Fluorescent Carbon Nanoparticles for Tumor Selective Theranostics via pH-turn on/off Fluorescence and Photothermal Effect In Vivo and In Vitro
Description
The new nanoparticle was developed by comprising a photothermal dye (IR825)-loaded carbonized zwitterionic polymer [FNP-I]. This nanoparticle act as a pH-responsive fluorescence probes to sense intracellular cancer cell and for near infrared (NIR) controllable photothermal therapy (PTT) after activation by reaching the cancer cell environment. In the in vitro and in vivo experiment, the nanoparticle showed good cellular uptake and biocompatibility as potential imaging-guided photothermal therapy nanotool for cancer treatment. In the study, the researchers identified the biodistribution inside the body at specific time points by in vivo imaging of whole mice after the intravenous injection of nanoparticle and confirm that the accumulation of nanoparticle in tumor tissue by ex vivo imaging of the organs after the sacrifice with Visque InVivo Elite.
Journal
Nanoscale 2018 Feb 1;10(5):2512-2523
Exendin-4 protects hindlimb ischemic injury by inducing angiogenesis 2015
Title
Exendin-4 protects hindlimb ischemic injury by inducing angiogenesis
Description
The angiogenic effect of exendin-4 using a mouse hindlimb ischemia model was analyzed with real time fluorescent imaging ability and feature map analysis abilities of Clevue, the SW of Vieworks.
Journal
Biochemical and Biophysical Research Communications.
2015 Oct2;465(4):758-63.
Age-related changes in pial arterial structure and blood flow in mice 2015
Title
Age-related changes in pial arterial structure and blood flow in mice
Description
Age-related changes in cerebral blood flow and vasculature of mice were investigated with real time fluorescent imaging ability and feature map analysis abilities of CleVue, the SW of Vieworks.
Journal
Neurobiology of Aging.
2015 Jan;37:161-170
Use of indocyanine green for optical analysis of cortical infarcts in photothrombotic ischemic brains 2015
Title
Use of indocyanine green for optical analysis of cortical infarcts in photothrombotic ischemic brains
Description
The photothrombotic ischemic injury of brain using mice were analyzed and diagnosed with real time imaging ability and FluAngio and DyAngio algorithm which was developed by collaborated research of Kyung Hee University and Vieworks.
CleVue enables equivalent analysis as described in this paper.
Journal
Biochemical and Biophysical Research Communications.
2015 Oct2;465(4):758-63.
Optical measurement of mouse strain differences in cerebral blood flow using indocyanine green 2015
Title
Optical measurement of mouse strain differences in cerebral blood flow using indocyanine green
Description
The difference of cerebral blood flow between two mouse strain was analyzed and compared with feature map ability which is developed by collaborated research of Kyung Hee University and Vieworks. CleVue enables equivalent analysis as described in this paper.
Journal
Journal of Cerebral Blood Flow & Metabolism.
2015 Jun;35(6):912-6
Noninvasive Optical Measurement of Cerebral Blood Flow in Mice Using Molecular Dynamics Analysis of Indocyanine Green 2012
Title
Noninvasive Optical Measurement of Cerebral Blood Flow in Mice Using Molecular Dynamics Analysis of Indocyanine Green
Description
The cerebral blood flow of mice was investigated by real time imaging ability with ICG and fluorescence dynamics analysis ability.
CleVue enables equivalent analysis as described in this paper.
Journal
PLOS ONE.
2012;7(10):e48383.
Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature 2011
Title
Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature
Description
The vasculature of tumor were imaged and analyzed by realtime fluorescence imaging with ICG and analysis algorithms.
CleVue enables equivalent analysis as described in this paper.
Journal
Journal of Biomedical Optics
2011 Apr;16(4):046008.
Efficient differentiation of human pluripotent stem cells into functional CD34 progenitor cells by combined modulation of the MEK/ERK and BMP4 signaling pathways 2010
Title
Efficient differentiation of human pluripotent stem cells into functional CD34 progenitor cells by combined modulation of the MEK/ERK and BMP4 signaling pathways
Description
The vasculogenic effects of stem cell therapy on ischemic tissue of mice were investigated by analyzing the blood flow of ischemic tissue.
Journal
BLOOD.
2010 Dec16;116(25):5762-72
Unsorted human adipose tissue-derived stem cells promote angiogenesis and myogenesis in murine ischemic hindlimb model 2010
Title
Unsorted human adipose tissue-derived stem cells promote angiogenesis and myogenesis in murine ischemic hindlimb model
Description
The promotion effect of angiogenesis and myogenesis in mouse ischemic hindlimb model after treating stem cells is identified by analyzing fluorescence image of tissue.
Journal
Microvascular Research.
2010 Dec;80(3):310-6.
Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics 2009
Title
Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics
Description
The relation between perfusion rate and necrosis was investigated with realtime fluorescence imaging and analysis in mouse ischemic hindlimb model.
Journal
PLOS One. 2009;4(1):e4275.
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