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VISQUE® InVivo Smart-LF

Compact Preclinical in vivo Fluorescent & Bioluminescent Imaging and Analysis System

High-sensitivity Bioluminescent & Fluorescent Imaging

Scientific CMOS Camera with Cooling Technology

  • Optimized solution for high-end scientific applications
  • Min. image pixel size: 26.5 µm
  • Cooling: -50℃ below ambient temperature

High-sensitivity sCMOS Image Sensor

  • Quantum efficiency: Max. 94% @ 550 nm
    >85% 450 – 700 nm
    >30% 300 – 950 nm
  • Readout noise: 1.6e-

Fast-speed Imaging Acquisition

  • Uniformed-quality image with high-speed image
    acquisition up to 37 frames per second
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Nude mouse image taken immediately after injection of
4T1-luc cell (2.5 x 10 6 ) into tail vein.

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Nude mouse image taken 6 hours after injection of
drug delivery material labeled with ICG into tail vein.

Intelligent in vivo Imaging Viewer & Kinetic Analysis Program

Fast and Convenient Analysis Tools

  • One-click analysis
    : automatic display of fluorescence level and simple setting
  • Image process
    : autofluorescence signal removal, merge of multi-spectral images
  • Report mode
    : the acquisition setup information, raw image, ROI, the range of pseudocolor bar, comments, etc.

Convenient Post-image Analysis and Edition Tools

  • *.cif analysis file output
  • The display and analysis of four-different images in one screen
  • Other supported formats
    : tif, bmp, jpg, png

Time-Lapse Imaging and Analysis Software

  • Supporting more than 10 analytic algorithms for pharmacokinetics and biodistribution by using the real-time image acquisition
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Refined Design & Optional Accessories to Enhance Usability

User-friendly Product Design

  • Light weight of 22 kg (possible to hand-carry)
  • Compact design
  • Foot switch
  • Sliding stage

Easy Lens Control to Obtain Precise Imaging

  • Motorized Iris / Zoom / Focus for detailed adjustments
  • Real-time observation of inside of the equipment
  • Zoom: 1x ~ 3x

Optional Accessories for Convenient Operation

  • Heating stage
  • Anesthesia ventilator adaptor (available in certain regions)
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In vivo Fluorescent and Bioluminescent Imaging

  • Tracking metastatic cancer cells with bioluminescent signal
  • Imaging solid tumor with fluorescent and/or bioluminescent signals
  • Assessment of cardiovascular and/or lymphatic structures and
    functions using fluorescent signal
  • Evaluation of therapeutic efficacy for new drugs and/or therapies against cancer,
    arthritis, atherosclerosis, autoimmune diseases and angiogenesis etc
  • Pharmacokinetic analysis of new drugs and/or drug delivery system.

Pharmacokinetics study of exosomes labeled with NIR fluorescent dyes

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0 hr: immediately after IP injection of the exosome-ICG complex.
The blue arrow indications the injection spot.

Representative Detectable Dyes

Representative Detectable Dyes
Imaging – Mode Imaging - Light Excitation / Emission Fluorescent Dyes
GFP Blue Ex : 390nm - 490nm
Em : 500nm - 550nm
GFP / EGFP / Alexa 488 / FITC / QD 525
PE Green Ex : 530nm - 570 nm
Em : 575nm - 640 nm
RFP / DsRed / PE / Alexa 568 / TRITC / QD 585 / QD 605 / QD 625
Cy5.5 Red Ex : 620nm - 650nm
Em : 690nm - 740nm
Cy5.5 / PKE680 / Alexa 680 / Alexa 700 / QD 705
HyperRed Ex : 630nm - 680nm
Em : 690nm - 740nm
ICG NIR Ex : 740nm - 790nm
Em : 810nm - 860nm
ICG / QD 800
Technical Specifications arrow
Technical Specifications
System
Dimension 40 cm x 40 cm x 57 cm
Weight 22 kg (48 lbs)
Operating Temperature 10℃ to 27℃
Power 100 – 240 V AC, 50/60 ㎐, max. 0.5 A at 220 V AC
Camera
Sensor 1.2” Backside Illuminated sCMOS
Resolution (H x V) 1824 X 1824
Pixel Size 6.5 um X 6.5 um
Min. Image Pixel Resolution 26.5 um (3x)
Digital Output 16 bit
Maximum Frame Rate 37 fps
Exposure Time 25 ms to 15 min
Detection Spectral Range 220 ㎚­ to 940 ㎚
Interface USB 3.0
Cooling -50℃ below ambient temperature, Thermoelectric Peltier Cooling
Lens
Control Motorized Iris / Zoom / Focus
Zoom (Field of View, H x V) 15 cm x 15 cm (1x) to 5 cm X 5 cm (3x)
Software, CleVueTM
Supported File Format cif(exclusive file format)
Supported Image File Format tif, bmp, jpg, png
Image Merging Merges images of multi-fluorescent dyes
Removal of Autofluorescence Removes autofluorescence or reflection from fluorescent images
Report Mode Displays an analyzed image with color scale bar, analyzed data, acquisition info, comments etc.
Kinetics Analysis • Includes 10 kinds of algorithms, i.e. MTT, BFI, and patented other algorithms to analyze Kinetics
• Dynamics graph, i.e. a plot of pixel intensity over time
• Map with Kinetics values on an image
Excitation Light
Source LED
White Light epi white LED
Emission Filters
Filter Selection Automated Control
Emission Filters Up to 9, optional
Stage
Stage Type Sliding stage, Up to 3 mice
Optional Accessory Heating stage, Anesthesia ventilator adaptor (available in certain regions)

* Specifications are subject to change without prior notice.

* This system is only for research

Citation Papers arrow
Title Publication (SCI) Link
A nanoscale photothermal agent based on a metal-organic
coordination polymer as a drug-loading framework for effective
combination therapy
Acta Biomater. 2019 Aug;94:435-446 link
Blood flow characteristics of diabetic patients with complications
detected by optical measurement
BioMedical Engineering OnLine. 2018 17: 25 link
Age-related changes in pial arterial structure and blood flow in mice Neurobiol Aging. 2016 Jan;37:161-170 link
Exendin-4 protects hindlimb ischemic injury by inducing angiogenesis Biochem Biophys Res Commun. 2015 Oct 2;465(4):758-63. link
Use of indocyanine green for optical analysis of cortical infarcts in
photothrombotic ischemic brains
J Neurosci Methods. 2015 Jun 15;248:46-50 link
Optical measurement of mouse strain differences in cerebral
blood flow using indocyanine green
J Cereb Blood Flow Metab. 2015 Jun;35(6):912-6 link
Noninvasive Optical Measurement of Cerebral Blood
Flow in Mice Using Molecular Dynamics Analysis of
Indocyanine Green
PLoS One. 2012;7(10):e48383. link
Efficient differentiation of human pluripotent stem cells into
functional CD34 + progenitor cells by combined modulation of the
MEK/ERK and BMP4 signaling pathways
Blood. 2010 Dec 16;116(25):5762-72  link
Unsorted human adipose tissue-derived stem cells promote
angiogenesis and myogenesis in murine ischemic hindlimb model
Microvasc Res. 2010 Dec;80(3):310-6. link
Quantitative Analysis of Peripheral Tissue Perfusion
Using Spatiotemporal Molecular Dynamics
PLoS One. 2009;4(1):e4275. link
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