VISQUE® InVivo Smart-LF
Compact Preclinical in vivo Fluorescent & Bioluminescent Imaging and Analysis System
Features
High-sensitivity Bioluminescent & Fluorescent Imaging
Scientific CMOS Camera with Cooling Technology
- Optimized solution for high-end scientific applications
- Min. image pixel size: 26.5 µm
- Cooling: -20℃
High-sensitivity sCMOS Image Sensor
- Quantum efficiency:
Max. 94% @ 550 nm
>85% 450 nm – 700 nm
>30% 300 nm – 950 nm - Readout noise: 1.6e-
Fast-speed Imaging Acquisition
- Uniformed-quality image with high-speed image
acquisition up to 37 frames per second
LED light source
- Employs up to 5 LEDs: Blue, Green, Red, HyperRed, N750
- Strong light power due to concentrated light source energy on a specific wavelength
- LED-based spectral unmixing
- Immediate use (no warm-up/cool-down time)
- Long lifespan (10,000 - 50,000 hours)
- High SNR (signal to noise ratio) due to weak reflection (of excitation light) in the wavelength of emission filter in fluorescence imaing
Nude mouse image taken immediately after injection of
4T1-luc cell (2.5 x 10 6 ) into tail vein.
Nude mouse image taken 6 hours after injection of
drug delivery material labeled with ICG into tail vein.
Intelligent in vivo Imaging Viewer & Kinetic Analysis Program
Fast and Convenient Analysis Tools
- One-click analysis
: automatic display of fluorescence level and simple setting - Image process
: autofluorescence signal removal, merge of multi-spectral images - Report mode
: the acquisition setup information, raw image, ROI, the range of pseudocolor bar, comments, etc.
Convenient Post-image Analysis and Edition Tools
- *.cif analysis file output
- The display and analysis of four-different images in one screen
- Other supported formats
: tif, bmp, jpg, png
Time-Lapse Imaging and Analysis Software
- Supporting more than 10 analytic algorithms for pharmacokinetics and biodistribution by using the real-time image acquisition
Refined Design & Optional Accessories to Enhance Usability
User-friendly Product Design
- Light weight of 22 kg (possible to hand-carry)
- Compact design
- Foot switch
- Sliding stage
Easy Lens Control to Obtain Precise Imaging
- Motorized Iris / Zoom / Focus for detailed adjustments
- Real-time observation of inside of the equipment
- Zoom: 1x - 3x
Optional Accessories for Convenient Operation
- Heating stage
- Anesthesia ventilator adaptor
In vivo Fluorescent and Bioluminescent Imaging
- Tracking metastatic cancer cells with bioluminescent signal
- Imaging solid tumor with fluorescent and/or bioluminescent signals
- Assessment of cardiovascular and/or lymphatic structures and
functions using fluorescent signal - Evaluation of therapeutic efficacy for new drugs and/or therapies against cancer,
arthritis, atherosclerosis, autoimmune diseases and angiogenesis etc - Pharmacokinetic analysis of new drugs and/or drug delivery system.
Pharmacokinetics study of exosomes labeled with NIR fluorescent dyes
0 hr: immediately after IP injection of the exosome-ICG complex.
The blue arrow indicates the injection spot.
Representative Detectable Dyes
Imaging – Mode | Imaging - Light | Excitation / Emission | Fluorescent Dyes |
---|---|---|---|
GFP | Blue | Ex : 390 nm - 490 nm Em : 500 nm - 550 nm |
GFP / EGFP / Alexa 488 / FITC / QD 525 |
PE | Green | Ex : 530 nm - 570 nm Em : 575 nm - 640 nm |
RFP / DsRed / PE / Alexa 568 / TRITC / QD 585 / QD 605 / QD 625 |
Cy5.5 | Red | Ex : 620 nm - 650 nm Em : 690 nm - 740 nm |
Cy5.5 / PKE680 / Alexa 680 / Alexa 700 / QD 705 |
HyperRed | Ex : 630 nm - 680 nm Em : 690 nm - 740 nm |
||
ICG | NIR | Ex : 740 nm - 790 nm Em : 810 nm - 860 nm |
ICG / QD 800 |
System | |
---|---|
Dimension | 40 cm x 40 cm x 57 cm |
Weight | 22 kg (48 lbs) |
Operating Temperature | 10℃ to 27℃ |
Power | 100 – 240 V AC, 50/60 ㎐, max. 0.5 A at 220 V AC |
Camera | |
Sensor | 1.2” Backside Illuminated sCMOS |
Resolution (H x V) | 1824 X 1824 |
Pixel Size | 6.5 um X 6.5 um |
Min. Image Pixel Resolution | 26.5 um (3x) |
Digital Output | 16 bit |
Maximum Frame Rate | 37 fps |
Exposure Time | 25 ms to 15 min |
Detection Spectral Range | 220 ㎚ to 940 ㎚ |
Interface | USB 3.0 |
Cooling | -20℃, Thermoelectric Peltier Cooling |
Lens | |
Control | Motorized Iris / Zoom / Focus |
Zoom (Field of View, H x V) | 15 cm x 15 cm (1x) to 5 cm X 5 cm (3x) |
Software, CleVueTM | |
Supported File Format | cif(exclusive file format) |
Supported Image File Format | tif, bmp, jpg, png |
Image Merging | Merges images of multi-fluorescent dyes |
Removal of Autofluorescence | Removes autofluorescence or reflection from fluorescent images |
Report Mode | Displays an analyzed image with color scale bar, analyzed data, acquisition info, comments etc. |
Kinetics Analysis | • Includes 10 kinds of algorithms, i.e. MTT, BFI, and patented other algorithms to analyze Kinetics • Dynamics graph, i.e. a plot of pixel intensity over time • Map with Kinetics values on an image |
Excitation Light | |
Source | LED |
White Light | epi white LED |
Emission Filters | |
Filter Selection | Automated Control |
Emission Filters | Up to 9, optional |
Stage | |
Stage Type | Sliding stage, Up to 3 mice |
Optional Accessory | Heating stage, Anesthesia ventilator adaptor (available in certain regions) |
* Specifications are subject to change without prior notice.
* This system is only for research