논문 | 바이오 영상 | 뷰웍스(Vieworks)

Regulation of cerebral blood flow boosts precise brain targeting of vinpocetine-derived ionizable-lipidoid nanoparticles 2024 in vivo System

제목: Regulation of cerebral blood flow boosts precise brain targeting of vinpocetine-derived ionizable-lipidoid nanoparticles
상세 내용: In this study, researchers demonstrated that vinpocetine-derived ionizable-lipidoid nanoparticles (VIP) effectively enhance brain targeting. In the brain-targeting experiment, LNP@DiD was administered to healthy mice via the tail vein. Fluorescence images were captured at specified time intervals (0.5 h, 1 h, 2 h, 4 h, and 8 h) using the VISQUE in vivo Smart-LF System. The nanoparticles containing VIP exhibited significantly higher fluorescence intensity in the brain compared to those without VIP, confirming their robust brain-targeting capability. Additionally, major organs were collected from the mice at each time point for ex vivo imaging. Mice treated with VIP showed stronger fluorescence signals in the brain compared to the positive control, GSH-LNP, which utilized glutathione (GSH) as the targeting agent.
학술지: Nat Commun. 2024; 15: 3987.

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BMP7-Loaded Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorate Liver Fibrosis by Targeting Activated Hepatic Stellate Cells 2024 in vivo System

제목: BMP7-Loaded Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorate Liver Fibrosis by Targeting Activated Hepatic Stellate Cells
상세 내용: "The study focuses on enhancing the healing properties of small extracellular vesicles derived from human umbilical cord mesenchymal stem cells (hucMSC-sEVs). Due to their tendency to non-specific distribution in vivo and be partially removed by monocular macrophages, natural hucMSC-sEVs are not fully effective when administered systemically. To address this, researchers have modified these vesicles at the genetic level to overexpress the anti-fibrotic gene bone morphogenic protein7(BMP7) in partner cell.
In study, two mice from each group received injections of different types of small extracellular vesicles (sEVs) into their tail veins. The researchers tracked the sEVs’ movement through the body using the VISQUE in vivo system at various intervals up to 72 hours after injection. The imaging revealed that the BMP7-loaded sEVs were effectively directed to the liver of the mice. These results demonstrated hucMSC-sEVs primarily accumulated in the liver and had a moderate ability to target activated hepatic stellate cells (aHSCs), which was further improved by incorporating BMP7 into the sEVs."
학술지: Int J Nanomedicine. 2024; 19: 3475–3495.

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Role of gut-derived bacterial lipopolysaccharide and peripheral TLR4 in immobilization stress-induced itch aggravation in a mouse model of atopic dermatitis 2024 in vivo System

제목: Role of gut-derived bacterial lipopolysaccharide and peripheral TLR4 in immobilization stress-induced itch aggravation in a mouse model of atopic dermatitis
상세 내용: This study elucidates the mechanisms by which emotional stress exacerbates itch sensation and inflammation in the skin of patients with atopic dermatitis (AD). The researchers investigate the role of gut-derived bacterial components, specifically lipopolysaccharides (LPS), in this process, focusing on how chronic stress impacts intestinal barrier function and its subsequent effects on skin inflammation and itch in a mouse model of AD. VISQUE InVivo Smart LF was employed to visualize the whole-body distribution of Cy5.5-labeled bacterial LPS. On the final day of the daily immobilization stress (IMO) period, nude mice were anesthetized, and their rectums were isolated. Cy5.5-labeled LPS was injected into the proximal colon, and the abdominal wall was sutured. The imaging showed how bacterial LPS permeates and distributes in the body under chronic stress conditions, providing insights into the role of gut-derived LPS in exacerbating skin inflammation and itch in the context of chronic stress and AD.
학술지: Sci Rep. 2024; 14: 6263.

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Modulation of chronic obstructive pulmonary disease progression by antioxidant metabolites from Pediococcus pentosaceus: enhancing gut probiotics abundance and the tryptophan-melatonin pathway 2024 in vivo System

제목: Modulation of chronic obstructive pulmonary disease progression by antioxidant metabolites from Pediococcus pentosaceus: enhancing gut probiotics abundance and the tryptophan-melatonin pathway
상세 내용: "This study focused on evaluating the therapeutic potential of probiotics for Chronic obstructive pulmonary disease (COPD), a condition closely associated with oxidative stress. They tested the probiotic Pediococcus pentosaceus SMM914 could alleviate COPD symptoms and slow disease progression.
The study used VISQUE InVivo Smart and CleVue software for NIR fluorescence imaging to track the colonization of the probiotic SMM914 in the body. Using Cy5.5-d-lys labeled SMM914 mouse measured at 15 min, 45 min, and 4 hours after oral administration. The results showed that 45 minutes post-administration, most of the bacteria were located in the jejunum. After 2.5 hours, the bacteria concentrated in the ileum and colon, and by 4 hours, the fluorescence was primarily in the ileum, suggesting that the bacteria were nearing excretion. Additionally, the supplementation with SMM914 not only increased serotonin production but also improved the activity of the DDC enzyme in an inflammatory setting, supporting the probiotic's potential therapeutic effects in managing COPD."
학술지: Gut Microbes. 2024; 16(1): 2320283.

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Promoting collateral formation in type 2 diabetes mellitus using ultra-small nanodots with autophagy activation and ROS scavenging 2024 in vivo System

제목: Promoting collateral formation in type 2 diabetes mellitus using ultra-small nanodots with autophagy activation and ROS scavenging
상세 내용: The study investigates the potential of molybdenum disulfide nanodots (MoS2 NDs) in promoting collateral formation and improving prognosis in type 2 diabetes mellitus (T2DM) by enhancing endothelial autophagy and scavenging reactive oxygen species (ROS). The Vieworks in vivo flourescence imaging system was used to assess the biodistribution of Cy5.5-labeled MoS2 NDs in ischemic muscles of a hindlimb ischemia mouse model to observe how these nanodots localize in the body post-injection. The imaging showed successful biodistribution of MoS2 NDs to the ischemic sites, indicating their effective delivery to targeted areas in the body
학술지: J Nanobiotechnology. 2024; 22: 85.

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Pharmaceutical Manipulation of Mitochondrial F0F1‐ATP Synthase Enables Imaging and Protection of Myocardial Ischemia/Reperfusion Injury Through Stress‐induced Selective Enrichment 2023 in vivo System

제목: Pharmaceutical Manipulation of Mitochondrial F0F1‐ATP Synthase Enables Imaging and Protection of Myocardial Ischemia/Reperfusion Injury Through Stress‐induced Selective Enrichment
상세 내용: This study aims to assess the ability of IR-780, a near-infrared dye, to image and protect myocardial tissue from ischemia/reperfusion (I/R) injury by selectively targeting the injured cardiomyocytes. VISQUE In Vivo Smart-LF was employed to visualize IR-780’s accumulation in myocardial tissue in a pig I/R model, aiding in the real-time imaging of the injury site. The imaging demonstrated selective retention of IR-780 in ischemic areas, allowing for effective visualization of at-risk tissue and showing a reduction in I/R-induced cell death, which suggests potential cardioprotective effects.
학술지: Adv Sci (Weinh). 2023 Dec 14;11(9):2307880.

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Imaging assessment of photosensitizer emission induced by radionuclide-derived Cherenkov radiation using charge-coupled device optical imaging and long-pass filters 2023 in vivo System

제목: Imaging assessment of photosensitizer emission induced by radionuclide-derived Cherenkov radiation using charge-coupled device optical imaging and long-pass filters
상세 내용: This study seeks to find a cost-effective method to confirm the excitation of photosensitizers (PS) by radionuclide-derived Cherenkov radiation (CR) and distinguish the resulting fluorescence emission. Tetrakis (4-carboxyphenyl) porphyrin (TCPP) was selected as a model PS, and the beta-emitting isotope Copper-64 was used as a CR-producing radionuclide. VISQUE InVivo Smart was used to confirm the inherent fluorescence characteristics of TCPP emission with filters for blue light (390-490 nm) excitation and red light (690-740 nm) emission. Combining other experiments, the researchers conclude that a simple method utilizing a CCD optical imaging system with different high-transmission long-pass filters and subtraction image processing can effectively assess if photosensitizer candidates are excited by radionuclide-derived Cherenkov radiation to generate fluorescence emission.
학술지: World J Radiol. 2023 Nov 28; 15(11): 315–323.

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Development of a fibroblast activation protein-targeted PET/NIR dual-modality probe and its application in head and neck cancer 2023 in vivo System

제목: Development of a fibroblast activation protein-targeted PET/NIR dual-modality probe and its application in head and neck cancer
상세 내용: This study developed the PET/NIR probe [68Ga]Ga-FAP-2286-ICG, targeting fibroblast activation protein (FAP) found in cancer-associated fibroblasts in solid tumors like head and neck squamous cell carcinoma (HNSCC). Fluorescence imaging using the VISQUE InVivo Smart-LF confirmed the probe's effectiveness in guiding surgical resections for precise tumor removal. U87MG tumor-bearing mice were imaged post-injection to identify the optimal timing for fluorescence imaging (FI), showing significant contrast as early as 0.5 hours, peaking at 24 hours and remaining elevated until 72 hours, indicating the ideal window for surgery. Unlike free ICG, which showed no tumor fluorescence, FAP-2286-ICG demonstrated enhanced targeting and retention. The imaging also revealed significant fluorescence in the liver and kidneys, indicating metabolic processing, and residual fluorescence post-surgery highlighted its potential for detecting remaining cancerous tissues.
학술지: Front Bioeng Biotechnol. 2023 Nov 3;11:1291824.

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Rapid and Widespread Distribution of Intranasal Small Extracellular Vesicles Derived from Mesenchymal Stem Cells throughout the Brain Potentially via the Perivascular Pathway 2023 in vivo System

제목: Rapid and Widespread Distribution of Intranasal Small Extracellular Vesicles Derived from Mesenchymal Stem Cells throughout the Brain Potentially via the Perivascular Pathway
상세 내용: The study aims to explore the distribution and potential therapeutic benefits of small extracellular vesicles (sEVs) derived from mesenchymal stem cells (MSCs) when administered intranasally, emphasizing their rapid and extensive delivery through the perivascular pathway. A VISQUE In Vivo Smart-LF system was utilized to image the distribution of Cy7-labeled MSC-sEVs in mice at 0.5, 2, 6, and 24 hours post-intranasal administration. Additionally, ex vivo imaging was performed to compare delivery to different organs. This data confirmed the fast and centralized distribution of intranasal MSC-sEVs.
학술지: Pharmaceutics. 2023 Nov; 15(11): 2578.

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Exosomal mRNAs for Angiogenic–Osteogenic Coupled Bone Repair 2023 in vivo System

제목: Exosomal mRNAs for Angiogenic–Osteogenic Coupled Bone Repair
상세 내용: The main goal of the study is to develop a scalable and efficient method for producing therapeutic small extracellular vesicles (sEVs) loaded with mRNAs for bone tissue regeneration. The Vieworks VISQUE in vivo Smarr-LF was employed to perform fluorescence imaging of PKH26-labeled sEVs within the PEGS-A hydrogel and standard scaffolds in a rat model. The imaging showed strong PKH26 signals, indicating successful delivery and distribution of the sEVs. Notably, the PEGS-A hydrogel demonstrated significantly higher fluorescence intensity compared to the standard scaffold after three days. This imaging confirmed that the sEVs were retained and released more effectively within the hydrogel, leading to sustained therapeutic presence at the defect site over a two-week period.
학술지: Adv Sci (Weinh). 2023 Oct 17;10(33):2302622.

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Sustained delivery of triamcinolone acetonide from a thermosensitive microemulsion gel system for the treatment of sensorineural hearing loss 2023 in vivo System

제목: Sustained delivery of triamcinolone acetonide from a thermosensitive microemulsion gel system for the treatment of sensorineural hearing loss
상세 내용: The study aims to develop and evaluate a thermosensitive microemulsion gel (MEG) system for the sustained delivery of triamcinolone acetonide (TA) to treat sensorineural hearing loss (SNHL) by improving drug retention and therapeutic efficacy in the inner ear. The VISQUE InVivo Smart-LF system was used to monitor the in vivo distribution and retention of Cy7-labeled TA-MEG in the inner ear of mice after intratympanic administration. This imaging system helped track the gel's localization and persistence over time. Imaging results demonstrated that the TA-MEG formulation exhibited sustained retention in the cochlea, with significant localization observed up to 6 days post-administration. This prolonged presence indicated effective gelation and slow degradation, validating the MEG system's potential for sustained drug delivery in SNHL treatment.
학술지: Drug Deliv. 2023; 30(1): 2242003.

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Site-selective superassembly of biomimetic nanorobots enabling deep penetration into tumor with stiff stroma 2023 in vivo System

제목: Site-selective superassembly of biomimetic nanorobots enabling deep penetration into tumor with stiff stroma
상세 내용: The primary objective of the study is to develop and evaluate a new class of nanorobots designed to improve tumor penetration and drug delivery for treating triple-negative breast cancer (TNBC). The VISQUE InVivo Elite was used to monitor tumor growth and evaluate the distribution of UHHTN(Urchin Head/Hollow Tail Nanostructures)/DOX(Doxorubicin) nanorobots in mouse models. Researchers conducted bioluminescence imaging to monitor tumor progression and performed ex vivo imaging of various organs at different time intervals after the nanorobot injection. This imaging approach provided detailed insights into the accumulation of the nanorobots in the tumor compared to other tissues, confirming their targeted delivery and enhancing understanding of their therapeutic efficacy. The results showed that the UHHTN/DOX nanorobots, combined with photothermal therapy, significantly reduced tumor growth and improved survival rates in treated mice, demonstrating their potential for effective cancer treatment.
학술지: Nat Commun. 2023 Aug 2;14:4628.

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Activation of autophagy by in situ Zn2+ chelation reaction for enhanced tumor chemoimmunotherapy 2023 in vivo System

제목: Activation of autophagy by in situ Zn2+ chelation reaction for enhanced tumor chemoimmunotherapy
상세 내용: The main goal of this study is to develop a nanoplatform that activates autophagy through in situ zinc ion (Zn²⁺) chelation, aiming to enhance the efficacy of tumor chemoimmunotherapy. VISQUE InVivo Elite was used to monitor the biodistribution and metabolism of Cy5.5-labeled DSF@Zn-DMSNs in tumor-bearing mice at various time points post-administration. Imaging results showed that the DSF@Zn-DMSNs had widespread and prolonged retention in tumor tissues. This retention confirmed the effective targeting and accumulation of the nanoparticles within the tumor, which correlated with the enhanced therapeutic outcomes observed in the study, including significant tumor growth inhibition and improved immune responses.
학술지: Bioact Mater. 2023 Nov; 29: 116–131.

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Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics 2023 in vivo System

제목: Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics
상세 내용: The study aimed to investigate the herapeutic potential of tumor-resident bacteria and their association with photosynthetic bacteria for targeted cancer immunotherapy. VISQUE InVivo Smart-LF was employed to monitor the near-infrared fluorescence (NIR FL) emitted by the bacteria in tumor-bearing mice, providing insights into their targeting efficacy and biodistribution. Mice received intravenous injections of bacterial suspensions, and imaging was performed five days post-injection to visualize the NIR FL intensity across the entire body, specifically focusing on tumor sites. The results indicated a strong tumor-targeting effect, with significant fluorescence observed in targeted tumors compared to other tissues, highlighting the effectiveness of the bacterial treatments in suppressing tumor growth and inducing durable antitumor immunity. Overall, the imaging demonstrated that these bacteria could serve as a promising therapeutic strategy for cancer treatment.
학술지: Adv Sci (Weinh). 2023 May 7;10(20):2301679.

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Synergistic Encapsulation of Paclitaxel and Sorafenib by Methoxy Poly(Ethylene Glycol)-b-Poly(Caprolactone) Polymeric Micelles for Ovarian Cancer Therapy 2023 in vivo System

제목: Synergistic Encapsulation of Paclitaxel and Sorafenib by Methoxy Poly(Ethylene Glycol)-b-Poly(Caprolactone) Polymeric Micelles for Ovarian Cancer Therapy
상세 내용: The main goal of this study is to develop and evaluate methoxy poly(ethylene glycol)-b-poly(caprolactone) (mPEG-b-PCL) polymeric micelles for the co-delivery of paclitaxel (PTX) and sorafenib (SRF) to enhance the treatment efficacy against ovarian cancer (OC) by exploiting the synergistic effects of these two drugs. Smart-LF was used in this study to monitor the in vivo bioluminescent imaging (BLI) of tumors in xenograft nude mice models. This system helped evaluate the tumor growth inhibition (TGI) percentage before and after drug administration. The imaging results using Smart-LF showed that the PTX/SRF micelle formulation exhibited significant tumor growth inhibition compared to the control and other treatment groups. The bioluminescence signal intensity measurements indicated effective suppression of tumor growth in the experimental groups treated with the micellar formulation.
학술지: Pharmaceutics. 2023 Apr; 15(4): 1206.

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Efficacy of recombinant Bacillus Calmette-Guérin containing dltA in in vivo three-dimensional bio-printed bladder cancer-on-a-chip and ex vivo orthotopic mouse model 2023 in vivo System

제목: Efficacy of recombinant Bacillus Calmette-Guérin containing dltA in in vivo three-dimensional bio-printed bladder cancer-on-a-chip and ex vivo orthotopic mouse model
상세 내용: The main goal of the paper is to evaluate the efficacy and optimal dosage of recombinant Bacillus Calmette-Guérin containing dltA (rBCG-dltA) in treating bladder cancer, using both a three-dimensional bio-printed bladder cancer-on-a-chip model and an orthotopic bladder cancer mouse model. The Vieworks VISQUE InVivio Smart-LF was used to monitor bioluminescence in the mouse bladder, enabling researchers to observe tumor progression and regression after treatment with rBCG-dltA. Smart-LF demonstrated that the rBCG-dltA treatment, particularly at higher dosages, significantly reduced tumor intensity compared to controls, indicating its potential antitumor efficacy(icu-64-296).
학술지: Investig Clin Urol. 2023 Mar 29;64(3):296–305

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Wavelength engineerable porous organic polymer photosensitizers with protonation triggered ROS generation 2023 in vivo System

제목: Wavelength engineerable porous organic polymer photosensitizers with protonation triggered ROS generation
상세 내용: The primary objective of this research is to develop a wavelength-engineerable, protonation-triggered porous organic polymer (POP) that can efficiently generate reactive oxygen species (ROS) for applications in biomedicine and photocatalysis. The focus is on creating a novel imidazolium-based POP that facilitates ROS generation without relying on metal ions or heavy atoms, addressing the challenge of maintaining high ROS yield while allowing for tunable excitation wavelengths. VISQUE InVivo Elite was used for the fluorescence imaging of U87MG-xenograft mice. The mice were imaged 24 hours after they were administered PBS, KUP-1, and O2@KUP-1 to assess the biodistribution and effectiveness of the developed photosensitizer. The imaging data indicated that O2@KUP-1 showed enhanced fluorescence signals compared to KUP-1 and PBS, demonstrating the effectiveness of the POP in generating ROS in vivo. This result underscores the potential of the developed polymer for applications in targeted cancer therapies and other biomedical fields.
학술지: Nat Commun. 2023; 14: 1498.

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Novel NF-κB reporter mouse for the non-invasive monitoring of inflammatory diseases 2023 in vivo System

제목: Novel NF-κB reporter mouse for the non-invasive monitoring of inflammatory diseases
상세 내용: "The main goal of the study was to create and evaluate a novel NF-kB luciferase reporter mouse model that enables non-invasive, real-time tracking of inflammatory responses in whole-body and cell-specific contexts.
The Vieworks VISQUE InVivo ART100 imaging system was used to capture bioluminescence signals in the mice, allowing researchers to visualize inflammation progression in different models, such as those induced by lipopolysaccharide (LPS) or diet. The result showed increased bioluminescence in targeted tissues correlating with inflammatory activity, validating the reporter mice’s capacity to reflect inflammation accurately and dynamically."
학술지: Sci Rep. 2023 Mar 2;13:3556.

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Bone tumor-homing nanotherapeutics for prolonged retention in tumor microenvironment and facilitated apoptotic process via mevalonate pathway inhibition 2023 in vivo System

제목: Bone tumor-homing nanotherapeutics for prolonged retention in tumor microenvironment and facilitated apoptotic process via mevalonate pathway inhibition
상세 내용: This paper aims to create and assess bone tumor-targeting polymeric nanotherapeutics (NTs) designed to enhance drug retention within the tumor microenvironment (TME) and improve the effectiveness of bone cancer treatments. The research involves the use of alendronate-decorated chondroitin sulfate A-graft-poly(lactide-co-glycolide) (PLCSA-AD) combined with the chemotherapy drug doxorubicin (DOX) to inhibit the mevalonate pathway, a key metabolic process in tumor cells, thereby inducing apoptosis (cell death) and enhancing therapeutic outcomes. VISQUE InVivo Smart was employed to visualize and verify the accumulation of PLCSA-AD nanotherapeutics in bone tumor tissue in a mouse xenograft model. Cy5.5-labeled NTs were injected into the tail vein of tumor-bearing mice, with imaging performed at 0, 1, 3, 5, 9, and 24 hours post-injection. Subsequently, the tumors and major organs were dissected for ex vivo analysis. The imaging results revealed a significant increase in tumor accumulation of the PLCSA-AD nanotherapeutics, showing a 2.64-fold higher radiant efficiency at 24 hours and a 1.73-fold higher tumor disposition ex vivo compared to the non-alendronate-decorated variant (PLCSA).
학술지: Mater Today Bio. 2023 Apr; 19: 100591.

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Endocrine modulation of brain-skeleton axis driven by neural stem cell-derived perilipin 5 in the lipid metabolism homeostasis for bone regeneration 2023 in vivo System

제목: Endocrine modulation of brain-skeleton axis driven by neural stem cell-derived perilipin 5 in the lipid metabolism homeostasis for bone regeneration
상세 내용: "The primary aim of the paper is to explore how neural stem cell-derived exosomes, particularly those containing perilipin 5 (PLIN5), contribute to lipid metabolism regulation to enhance bone regeneration via an endocrine axis linking the brain and skeleton.
The Vieworks VISQUE InVivo Elite imaging system was used to track fluorescently labeled neural stem cell-derived exosomes as they traveled from the brain to bone injury sites, allowing researchers to visualize their distribution and homing capabilities. Results confirmed that the exosomes reached the bone injury areas, highlighting their potential to aid bone regeneration by modulating lipid metabolism in metabolic disorder conditions."
학술지: Mol Ther. 2023 Feb 9;31(5):1293–1312.

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Overcoming the On‐Target Toxicity in Antibody‐Mediated Therapies via an Indirect Active Targeting Strategy 2023 in vivo System

제목: Overcoming the On‐Target Toxicity in Antibody‐Mediated Therapies via an Indirect Active Targeting Strategy
상세 내용: The primary objective of this study is to address and mitigate the on-target toxicity that is often associated with antibody-mediated therapy, specifically targeting tumor-associated antigens. The researchers aim to develop a method to reduce the adverse effects of antibody treatment by using a strategy that involves masking the antibody's binding domain with a cleavable peptide, which is then selectively removed in the tumor microenvironment. This approach seeks to enhance the specificity and safety of antibody-mediated cancer therapies. VISQUE in vivo Smart-LF was used for the ex vivo images of the fluorescently labeled antibodies. This allowed for temporal observation of how the antibodies circulated and accumulated in the tumors versus normal tissues, providing critical data on the effectiveness of the masking strategy. The images demonstrated that the engineered antibodies, with the masking peptide, showed reduced accumulation in non-tumor tissues compared to the unmodified antibodies. This selective localization was crucial to confirm that the peptide masking approach effectively reduced on-target toxicity without compromising the antibody's ability to target tumors.
학술지: Adv Sci (Weinh). 2023 Mar; 10(9): 2206912.

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Repurpose dasatinib and quercetin: Targeting senescent cells ameliorates postmenopausal osteoporosis and rejuvenates bone regeneration 2023 in vivo System

제목: Repurpose dasatinib and quercetin: Targeting senescent cells ameliorates postmenopausal osteoporosis and rejuvenates bone regeneration
상세 내용: "This study demonstrated that the combination of dadatinib and quercetin (DQ) can effectively improve postmenopausal osteoporosis (PMO) and enhance born health. Osteoporosis is one of the prevalnet chronic disease in the elderly, especially postmenopausal women. Current treatments for PMO often have shown adverse effects. DQ has shown potential in treating various age-related conditions. However, its effectiveness in PMO was not investigated until now.
VISQUE InVivo ART100 used to capture quantify fluorescence intensity of the released substance in vivo. The device was used to take images of the rat femoral defect area at different time points (1st, 3rd, and 5th day) to monitor the release of the substances. The fluorescence intensity of the released quercetin (using calcein as a model) and BMP2 (modified with sulfo-cyanine7) was measured. This study confirms that DQ can ameliorate PMO."
학술지: Bioact Mater. 2023 Jul; 25: 13–28.

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Nanoformulation of the K-Ras(G12D)-inhibitory peptide KS-58 suppresses colorectal and pancreatic cancer-derived tumors 2023 in vivo System

제목: Nanoformulation of the K-Ras(G12D)-inhibitory peptide KS-58 suppresses colorectal and pancreatic cancer-derived tumors
상세 내용: The main goal of the paper is to develop and evaluate a nanoformulation of the K-Ras(G12D)-inhibitory peptide KS-58 for its effectiveness in suppressing colorectal and pancreatic cancer-derived tumors. The researchers aimed to improve the delivery and efficacy of the KS-58 peptide, which targets the K-Ras(G12D) mutation, a common and challenging mutation in these types of cancers. Vieworks VISQUE InVivo Smart-LF was used to monitor and evaluate the tumor growth and the therapeutic effect of the KS-58 nanoformulation in live animal models. The imaging with VISQUE InVivo Smart-LF provided clear bioluminescent images that allowed the researchers to assess the effectiveness of the KS-58 nanoformulation. The results showed significant suppression of tumor growth in the treated groups compared to the control groups. The data obtained from the imaging system supported the study's goal by demonstrating that the nanoformulated KS-58 peptide effectively inhibits tumor growth in colorectal and pancreatic cancer models, thus validating the therapeutic potential of this approach.
학술지: Sci Rep. 2023; 13: 518.

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Potential Alzheimer's disease therapeutic nano-platform: Discovery of amyloid-beta plaque disaggregating agent and brain-targeted delivery system using porous silicon nanoparticles 2023 in vivo System

제목: Potential Alzheimer's disease therapeutic nano-platform: Discovery of amyloid-beta plaque disaggregating agent and brain-targeted delivery system using porous silicon nanoparticles
상세 내용: "This study introduces a new agent for breaking down amyloid-beta(Aβ) plaque and a targeted delivery system for Alzheimer's disease(AD) treatment usinf porous silicon nanoparticles(pSiNPs). The nano-formulation, named BiotiCaCl2-ANA-pSiNPs (BCAP), effectively delivered a significant amount of the therapeutic agent 6-amino-2-naphthalenesulfonic acid (ANA) to the AD brain. This led to the disaggregation of amyloid plaques and improved memory in an AD mouse model.
Fluorescence imaging with VISQUE InVivo Elit was used to monitor the distribution of BCAP in the body. The results indicated that BCAP accumulated significantly in the AD brain, demonstrating its superior brain-targeting capability. Imaging also confirmed that BCAP effectively disaggregated Aβ plaques in vivo, reducing their presence in the AD brain. The study demonstrated that BCAP disaggregated Aβ plaques with high efficacy in vitro and showed high biocompatibility in vivo, leading to improved memory and reduced inflammation."
학술지: Bioact Mater. 2023 Jun; 24: 497–506.

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DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma 2023 in vivo System

제목: DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma
상세 내용: The study investigates the role of diacylglycerol kinase B (DGKB) in the radioresistance of glioblastoma (GBM) cells. The researchers seek to understand how the downregulation of DGKB, which regulates the intracellular concentration of diacylglycerol (DAG), contributes to the resistance of GBM cells to radiotherapy. By exploring the mechanisms of DGKB downregulation and its effects on lipid metabolism, the study aims to identify potential therapeutic targets to overcome GBM radioresistance. VISQUE in vivo SmartLF was employed for bioluminescent imaging to track the tumor size and progression after irradiation in mice implanted with luciferase-expressing GBM cells. This imaging visualized the effects of radiotherapy combined with DGKB overexpression on tumor growth. It was revealed that radiation alone had minimal effects on tumor growth. However, when combined with DGKB overexpression, there was a significant reduction in tumor growth by approximately 62.19%.
학술지: Cell Rep Med. 2023 Jan 17; 4(1): 100880.

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Development of an alginate-gelatin bioink enhancing osteogenic differentiation by gelatin release 2023 in vivo System

제목: Development of an alginate-gelatin bioink enhancing osteogenic differentiation by gelatin release
상세 내용: This study aimed to enhance the osteogenic activity in a hydrogel by integrating gelatin, which supports both encapsulated and nearby cells. Methacrylate-modified alginate (MA-alginate) was selected for its low cell adhesion properties. VISQUE inVivo Smart system was used to monitor gelatin levels in hydrogel disks at several time points (0h, 1, 7, 14, and 21 days) using fluorescent imaging. By attaching fluorescein dye to gelatin, we assessed its release over time. The results showed that higher amounts of gelatin led to increased and gradual release of gelatin. This release improved the swelling properties, cell viability, and promote bone growth of the hydrogels compared to those made only of MA-alginate. Thus, the gelatin-containing alginate-based bioink developed could effectively support osteogenesis in bone tissue regeneration.
학술지: Int J Bioprint. 2023; 9(2): 660.

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Active Targeting of Versatile Nanocomplex Using the Novel Biomarker of Breast Cancer Stem Cells 2023 in vivo System

제목: Active Targeting of Versatile Nanocomplex Using the Novel Biomarker of Breast Cancer Stem Cells
상세 내용: The paper investigates the activation of autophagy through in situ Zn²⁺ chelation reaction to enhance tumor chemoimmunotherapy. The researchers explore how Zn²⁺ chelation can induce autophagy and subsequently improve the efficacy of chemotherapeutic drugs and immune responses against tumors. VISQUE in vivo Smart-LF is used to monitor and analyze the biodistribution of a Zn²⁺ chelator (ZnDPA) in mice. Fluorescent imaging of CT-26 tumor bearing BALB/c mice after 0, 2, 4, 8, 12, 24, 48, and 72h after the intratumoral injection of Cy5.5-labeld DSF@Zn-DMSNs revealed that ZnDPA exhibited significant tumor targeting capability, as evidenced by the accumulation of strong fluorescent signals in the tumor regions. This successful targeting highlights the potential of Zn²⁺ chelation for enhancing the delivery and effectiveness of chemotherapy and immunotherapy in cancer treatment.
학술지: Int J Mol Sci. 2023 Jan; 24(1): 685.

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Enhancement of the therapeutic efficacy of the MAP regimen using thiamine pyrophosphate‐decorated albumin nanoclusters in osteosarcoma treatment 2023 in vivo System

제목: Enhancement of the therapeutic efficacy of the MAP regimen using thiamine pyrophosphate‐decorated albumin nanoclusters in osteosarcoma treatment
상세 내용: "Current guidelines for Osteosarcoma recommend combination therapy rather than a single therapy. This study proposed that adding thiamine pyrophosphate (TPP) to nano-drug delivery systems could target bone tumors more effectively without increasing toxicity. The goal was to test whether replacing free methotrexate (MTX) and doxorubicin (DOX) with TPP-coated albumin nanoclusters could improve the effectiveness of the MAP regimen.
VISQUE InVivo Smart was used to monitor fluorescence signals from Cy5.5-labeled nanoclusters injected into mice. Whole-body body fluorescence was tracked using near-infrared fluorescence (NIRF) imaging at various time points: 0, 1, 2, 4, 6, 12, and 24 hours post-injection. After 24 hours, the system was also employed for ex vivo imaging of major organs and tumor tissues. The results showed that the albumin-based nanoclusters gradually accumulated in the osteosarcoma over time, with the TPP-decorated nanoclusters demonstrating improved tumor distributions. Thus, this study showed that the HSA(Human Serum Albumin)-TPP NCs-based MAP regimen may be a promising strategy for Osteosarcoma treatment."
학술지: Bioeng Transl Med. 2023 Nov; 8(6): e10472.

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Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy 2022 in vivo System

제목: Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy
상세 내용: The study aims to develop and evaluate a novel nanosystem that combines probiotics (Lactobacillus rhamnosus GG) with CRISPR-Cas9 technology to target and treat cancer. The researchers harness the tumor-targeting properties of probiotics and the gene-editing capabilities of CRISPR-Cas9 to specifically attack cancer cells in hypoxic tumor environments, improving the efficacy of cancer therapy. VISQUE imaging system is employed to track and analyze the accumulation and distribution of the nanosystem labeled with cy5.5 within tumor-bearing mice. This fluorescence imaging allowed for real-time observation of how well the nanosystem targeted and accumulated in tumor tissues. The imaging results showed that the hybrid nanosystem (LGG-MHS-Cas9) effectively accumulated in tumor tissues, particularly in hypoxic regions, while showing minimal off-target accumulation in other organs. These findings confirm the nanosystem’s potential for improving the precision and effectiveness of cancer therapies by concentrating the therapeutic agents in the areas where they are most needed.
학술지: Nat Commun. 2022; 13: 7903.

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Nanorod/nanodisk‐integrated liquid crystalline systems for starvation, chemodynamic, and photothermal therapy of cancer 2022 in vivo System

제목: Nanorod/nanodisk‐integrated liquid crystalline systems for starvation, chemodynamic, and photothermal therapy of cancer
상세 내용: "This study developed a hybrid gel indocyanine green (ICG), glucose oxidase (GOx), and copper(II) sulfate (Cu) with cellulose nanocrystal (CNC) and Laponite (LAP) was developed for targeted cancer therapy. The gel combines: Photothermal Therapy (PTT), Starvation Therapy (ST), Chemodynamic Therapy (CDT).
VISQUE InVivo Smart was used to evaluate ICG felease form the gel and its uptaken by CT-26 cancer cells, with fluorescence intensity measured at 0, 10, 30, 60, 120, 360, and 1440 min. The CNC/LAP gel with ICG effectively induces hyperthermia in tumors when exposed to near-infrared (NIR) laser, demonstrating improved retention and extended therapeutic effects. Overall, this gel shows promise for localized cancer treatment and maintains a high safety profile."
학술지: Bioeng Transl Med. 2023 Sep; 8(5): e10470.

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Modified exosomal SIRPα variants alleviate white matter injury after intracerebral hemorrhage via microglia/macrophages 2022 in vivo System

제목: Modified exosomal SIRPα variants alleviate white matter injury after intracerebral hemorrhage via microglia/macrophages
상세 내용: The study aims to develop and evaluate modified exosomal SIRPα variants (SIRPα-v Exos) to alleviate white matter injury after intracerebral hemorrhage (ICH) by promoting the clearance of hematoma and regulating the immune response via microglia/macrophages. VISQUE™ Imaging System was used to monitor the biodistribution of DiR-labeled SIRPα-v Exosomes in mice after intravenous injection. This system tracked the exosomes’ accumulation in various organs over time. Imaging results showed that SIRPα-v Exosomes accumulated in the brain and other organs, with a particularly notable presence in the brain 8 hours post-injection. This confirmed the ability of the exosomes to cross the blood-brain barrier and contribute to hematoma clearance.
학술지: Biomater Res. 2022 Nov 26;26:67.

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Folate receptor-targeted near-infrared photodynamic therapy for folate receptor-overexpressing tumors 2022 in vivo System

제목: Folate receptor-targeted near-infrared photodynamic therapy for folate receptor-overexpressing tumors
상세 내용: "Photodynamic therapy (PDT) is a promising minimally invasive cancer treatment that relies on photosensitizers (PS) and light exposure. Developing novel photosensitizers with optimal properties can significantly enhance PDT's effectiveness. Folate receptors (FR), which are overexpressed in about 40% of human cancers, are ideal targets for tumor-specific therapies. By conjugating photosensitizers with folate, specific targeting to FR can be achieved. The folate-linked near-infrared (NIR) probe, FolateSiR-1, has been developed to improve PDT by utilizing NIR light for targeted cancer treatment.
VISQUE InVivo Smart fluorescence imaging and analysis system was used to determine the fluorescence signal intensity (FI) of FilateSiR-1 in tumor-being mice, utilizing a HyperRed light filter set (Ex/Em = 630-680/690-740 nm). This system confirmed high fluorescence in KB tumors but not in OVCAR-3 or A4 tumors, indicating specific binding to folate receptors. Two hours post-injection, the fluorescence intensity peaked in KB tumors and diminished over time. In vivo and ex vivo imaging revealed significant tumor accumulation of FolateSiR-1 and reduced fluorescence in tumors subjected to photodynamic therapy (PDT), compared to untreated tumors. These results support the use of FolateSiR-1 as a promising photosensitizer for targeted PDT with minimal side effects."
학술지: World J Clin Oncol. 2022 Nov 24; 13(11): 880–895.

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ICAM1-Targeting Theranostic Nanoparticles for Magnetic Resonance Imaging and Therapy of Triple-Negative Breast Cancer 2022 in vivo System

제목: ICAM1-Targeting Theranostic Nanoparticles for Magnetic Resonance Imaging and Therapy of Triple-Negative Breast Cancer
상세 내용: The study aims to design and evaluate ICAM1-targeting theranostic nanoparticles for enhanced magnetic resonance imaging (MRI) and treatment of triple-negative breast cancer (TNBC). The researchers focus on developing nanoparticles that can specifically target TNBC for both imaging and therapeutic purposes. VISQUE InVivo Smart-LF is used to monitor the biodistribution of Cy5.5-labeled nanoparticles in TNBC-bearing mice at various time points (1, 2, 4, and 24 hours post-injection). The imaging showed that the ICAM1-targeting nanoparticles exhibited efficient tumor accumulation compared to the control group. The fluorescence imaging demonstrated prolonged circulation time and enhanced targeting of the nanoparticles to TNBC tumors, confirming their potential for both diagnostic imaging and therapeutic efficacy.
학술지: Int J Nanomedicine. 2022 Nov 22;17:5605–5619.

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Multiplexed Imaging Reveals the Spatial Relationship of the Extracellular Acidity-Targeting pHLIP with Necrosis, Hypoxia, and the Integrin-Targeting cRGD Peptide 2022 in vivo System

제목: Multiplexed Imaging Reveals the Spatial Relationship of the Extracellular Acidity-Targeting pHLIP with Necrosis, Hypoxia, and the Integrin-Targeting cRGD Peptide
상세 내용: "The study aimed to investigate the intratumoral distribution (ITD) of pH (low) insertion peptides (pHLIPs) and their spatial relationship with necrosis, hypoxia, and the integrin-targeting cRGD peptide in tumor models.
The VISQUE InVivo Smart-LF was used for in vivo and ex vivo near-infrared fluorescence (NIRF) imaging of tumor-bearing mice. The system helped visualize the distribution of IR800-pHLIP in the tumors at various time points post-injection. The imaging results showed that the ITD of pHLIPs was heterogeneous and had a high degree of colocalization with necrosis. The pHLIPs accumulated in necrotic regions and were spatially contiguous with hypoxia, providing insights into the potential theranostic applications of pHLIPs."
학술지: Cells. 2022 Nov 4;11(21):3499.

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NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma 2022 in vivo System

제목: NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma
상세 내용: The study aims to explore the role of NRBF2-mediated autophagy in glioblastoma (GBM) cells and how it contributes to metabolite replenishment and radioresistance. The researchers are trying to identify mechanisms through which autophagy can increase GBM cell survival during radiotherapy. With VISQUE Invivo Smart-LF, they perform bioluminescence imaging of tumor growth in GBM-bearing mice models. The researchers monitored tumor growth and assessed the effects of radiation on tumor development. The imaging showed that radiation treatment reduced tumor size, but tumors in the irradiated group exhibited higher expression of NRBF2 and proliferation markers, confirming its role in promoting radioresistance and tumor progression.
학술지: Exp Mol Med. 2022 Nov 4;54(11):1872–1885.

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Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment 2022 in vivo System

제목: Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment
상세 내용: "The study aims to investigate the role of shear-wave elasticity (SWE) imaging stiffness in predicting treatment responses for triple-negative breast cancer (TNBC), focusing on how cancer-associated fibroblasts (CAFs) influence tumor elasticity and the effectiveness of therapy.
The Vieworks VISQUE Smart-LF in vivo imaging system was employed to capture SWE images of TNBC tumors, assessing their stiffness as part of the non-invasive imaging technique used to correlate tumor elasticity with clinical outcomes. Imaging with the Vieworks device showed that TNBC tumors had higher SWE stiffness, linked to increased CAF activity, fibrosis, and tumor hypoxia, factors that could potentially predict a tumor's response to neoadjuvant therapies."
학술지: Bioact Mater. 2022 Nov 3;22:567–587.

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HA-DOPE-Modified Honokiol-Loaded Liposomes Targeted Therapy for Osteosarcoma 2022 in vivo System

제목: HA-DOPE-Modified Honokiol-Loaded Liposomes Targeted Therapy for Osteosarcoma
상세 내용: The paper aims to develop HA-DOPE-modified honokiol-loaded liposomes for targeted osteosarcoma therapy. The researchers seek to enhance the antitumor effects of honokiol (HNK) through improved delivery and targeting of osteosarcoma cells via interaction with the CD44 receptor. Using a VISQUE system, they observed the biodistribution of DiD-labeled liposomes in tumor-bearing mice. The fluorescence images showed that HA-DOPE-modified liposomes had significantly higher accumulation in the tumor compared to non-modified liposomes, demonstrating the effective targeting capability of the liposomal system to osteosarcoma cells via the CD44 receptor.
학술지: Int J Nanomedicine. 2022 Nov 1;17:5137–5151.

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Small molecule-assisted assembly of multifunctional ceria nanozymes for synergistic treatment of atherosclerosis 2022 in vivo System

제목: Small molecule-assisted assembly of multifunctional ceria nanozymes for synergistic treatment of atherosclerosis
상세 내용: "The primary objective of the paper is to investigate the potential of multifunctional ceria nanozymes, combined with small molecules, to treat atherosclerosis by simultaneously targeting reactive oxygen species (ROS) and inflammation within atherosclerotic lesions.
The study utilized the Vieworks VISQUE Elit/Smart in vivo imaging system to perform fluorescence imaging, helping visualize the distribution of the nanozymes within atherosclerotic plaques in mice. The result confirmed the effective accumulation of the platelet membrane-coated ceria nanozyme complexes within inflamed atherosclerotic areas, highlighting their targeted delivery and therapeutic potential."
학술지: Nat Commun. 2022 Nov 1;13:6528.

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Lipid Profiles Obtained from MALDI Mass Spectrometric Imaging in Liver Cancer Metastasis Model 2022 in vivo System

제목: Lipid Profiles Obtained from MALDI Mass Spectrometric Imaging in Liver Cancer Metastasis Model
상세 내용: The paper investigates lipid profiles in liver cancer metastasis using matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) to identify potential biomarkers for metastasis, thereby improving diagnosis and treatment strategies. VISQUE in vivo Elite is used to monitor liver cancer metastasis progression in mice via tracking the localization of SK-Hep1_Luc cells in mouse liver tissues. The imaging results confirmed the accumulation of metastatic lesions in the liver. This allowed researchers to monitor tumor progression and validate the experimental metastasis model.
학술지: Int J Anal Chem. 2022 Oct 27;2022:6007158

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Exosomal Plasminogen Activator Inhibitor-1 Induces Ionizing Radiation-Adaptive Glioblastoma Cachexia 2022 in vivo System

제목: Exosomal Plasminogen Activator Inhibitor-1 Induces Ionizing Radiation-Adaptive Glioblastoma Cachexia
상세 내용: "The study aims to elucidate the role of exosomal plasminogen activator inhibitor-1 (PAI-1) in inducing ionizing radiation-adaptive glioblastoma (GBM) cachexia and to explore the potential of targeting PAI-1 as a therapeutic strategy to manage GBM cachexia following radiotherapy.
VISQUE in vivo imaging system Smart-LF was used to monitor xenograft growth by bioluminescent imaging in the orthotopic xenograft mouse model. Results indicated that the total body weight and muscle fiber size of mice decreased after irradiation, indicating the development of cachexia in the irradiated GBM xenograft group."
학술지: Cells. 2022 Oct 1;11(19):3102.

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Huc-MSC-derived exosomes modified with the targeting peptide of aHSCs for liver fibrosis therapy 2022 in vivo System

제목: Huc-MSC-derived exosomes modified with the targeting peptide of aHSCs for liver fibrosis therapy
상세 내용: The study aims to develop Huc-MSC-derived exosomes modified with a targeting peptide (HSTP1) to specifically target activated hepatic stellate cells (aHSCs) for the treatment of liver fibrosis. The goal is to enhance the delivery efficiency and therapeutic effects of exosomes on reversing aHSC activation and mitigating liver fibrosis. VISQUE in vivo Smart is used to track and analyze the biodistribution of DiR-labeled exosomes in rats. The fluorescence signals in the abdominal liver region are monitored at various time points post-injection. The imaging results showed that the HSTP1-modified exosomes (HSTP1-Exos) exhibited stronger fluorescence signals in the liver, indicating higher targeting efficiency to the aHSC region compared to unmodified exosomes.
학술지: J Nanobiotechnology. 2022 Oct 1;20:432.

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Effect of Pinoresinol and Vanillic Acid Isolated from Catalpa bignonioides on Mouse Myoblast Proliferation via the Akt/mTOR Signaling Pathway 2022 in vivo System

제목: Effect of Pinoresinol and Vanillic Acid Isolated from Catalpa bignonioides on Mouse Myoblast Proliferation via the Akt/mTOR Signaling Pathway
상세 내용: This study investigates the effects of pinoresinol and vanillic acid, isolated from Catalpa bignonioides, on mouse myoblast proliferation, aiming to understand their potential in stimulating muscle growth via the Akt/mTOR signaling pathway. VISQUE InVivo Smart-LF was used to capture images of protein expression in Western blot analysis, allowing visualization of signaling pathway activation. Results showed that treatment with pinoresinol and vanillic acid notably increased protein phosphorylation within the Akt/mTOR pathway, supporting muscle cell proliferation and differentiation.
학술지: Molecules. 2022 Aug 24;27(17):5397

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Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma 2022 in vivo System

제목: Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma
상세 내용: The study investigates lenvatinib, a multi-kinase inhibitor, as a treatment option for nasopharyngeal carcinoma (NPC) that has developed resistance to antiangiogenic drugs (AADs). It aims to understand the mechanisms of AAD resistance and evaluate lenvatinib’s effectiveness in overcoming this resistance by targeting both VEGFR2 and FGFR1 pathways. VISQUE Invivo Elite is used to detect GFP-positive metastatic nodules in the lungs of NPC-bearing mice, providing detailed insights into tumor growth and metastasis. The imaging showed significant suppression of metastasis in NPC-bearing mice treated with lenvatinib compared to anti-VEGF treatments, confirming lenvatinib's effectiveness in targeting the tumor vasculature and reducing metastasis
학술지: Cell Death Dis. 2022 Aug 19;13(8):724.

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Reactive oxygen species‐responsive dual‐targeted nanosystem promoted immunogenic cell death against breast cancer 2022 in vivo System

제목: Reactive oxygen species‐responsive dual‐targeted nanosystem promoted immunogenic cell death against breast cancer
상세 내용: "The study aimed to develop a reactive oxygen species (ROS)-responsive dual-targeted nanosystem to promote immunogenic cell death (ICD) and enhance the therapeutic outcome against breast cancer.
The VISQUE InVivo imaging system was used to evaluate the tumor retention and targeting ability of the nanoparticles in a mouse xenograft model. The imaging results demonstrated that the nanoparticles displayed longer retention in the tumor zone and effectively accumulated in the tumor tissue, indicating their potential for targeted cancer therapy."
학술지: Bioeng Transl Med. 2022 Aug 3;8(5):e10379.

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Cardiomyocytes induced from hiPSCs by well-defined compounds have therapeutic potential in heart failure by secreting PDGF-BB 2022 in vivo System

제목: Cardiomyocytes induced from hiPSCs by well-defined compounds have therapeutic potential in heart failure by secreting PDGF-BB
상세 내용: The study explores the therapeutic potential of cardiomyocytes induced from human-induced pluripotent stem cells (hiPSCs) using well-defined compounds, focusing on their ability to secrete PDGF-BB and promote myocardial repair in heart failure. VISQUE InVivo Smart-LF was used to perform fluorescence imaging in mice to monitor the biodistribution and homing of transplanted hiPS-CMs over 20 days. The imaging demonstrated that the hiPS-CMs accumulated at the site of injury in the heart, with a longer duration of fluorescent signal observed for the compound-induced hiPS-CMs compared to the standard group, supporting their enhanced therapeutic efficacy.
학술지: Signal Transduct Target Ther. 2022 Jul 29;7:253.

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Exosomal miR-17-5p from adipose-derived mesenchymal stem cells inhibits abdominal aortic aneurysm by suppressing TXNIP-NLRP3 inflammasome 2022 in vivo System

제목: Exosomal miR-17-5p from adipose-derived mesenchymal stem cells inhibits abdominal aortic aneurysm by suppressing TXNIP-NLRP3 inflammasome
상세 내용: "The study aimed to investigate the role of exosomal miR-17-5p from adipose-derived mesenchymal stem cells (ADSCs) in inhibiting abdominal aortic aneurysm (AAA) progression by suppressing the TXNIP-NLRP3 inflammasome.
The VISQUE™ InVivo Smart-LF imaging system from Vieworks was used for in vivo fluorescence imaging to track the distribution of DR-labeled ADSC-derived exosomes in mice. Imaging results indicated that the exosomes were primarily localized in the liver, with notable accumulation also seen in the kidneys, lungs, and spleen."
학술지: Stem Cell Res Ther. 2022 Jul 26;13:349.

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Molecular imaging on ACE2‐dependent transocular infection of coronavirus 2022 in vivo System

제목: Molecular imaging on ACE2‐dependent transocular infection of coronavirus
상세 내용: The study investigates the ACE2-dependent transocular infection pathway of SARS-CoV-2, utilizing molecular imaging to provide dynamic, quantitative insights into how the virus invades and spreads within the body through the eye. VISQUE Smart-LF was employed for ex vivo fluorescence imaging to observe enhanced green fluorescent protein (EGFP) expression, which verified the presence of SARS-CoV-2 in the eyes and brain of treated mice. The imaging demonstrated significant EGFP expression in the eyes and brain of both humanized ACE2 (hACE2) and ACE2 knockout (ACE2-KO) mice, confirming transocular infection.
학술지: J Med Virol. 2022 Jul 5;94(10):4878–4889.

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Insulin-incubated palladium clusters promote recovery after brain injury 2022 in vivo System

제목: Insulin-incubated palladium clusters promote recovery after brain injury
상세 내용: The study aimed to synthesize insulin-incubated ultrasmall palladium clusters (Pd@insulin) and evaluate their therapeutic effects on traumatic brain injury (TBI) by scavenging reactive oxygen species (ROS). The VISQUE InVivo Smart-LF was used to trace the distribution of DR-labeled Pd@insulin clusters in mice. The imaging results indicated that Pd@insulin clusters were rapidly distributed throughout the body, with significant accumulation in the liver, kidney, spleen, and brain.
학술지: Biomacromolecules. 2022 Apr 22;23(8):3130–3141.

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High-Intensity Aerobic Exercise Suppresses Cancer Growth by Regulating Skeletal Muscle-Derived Oncogenes and Tumor Suppressors 2022 in vivo System

제목: High-Intensity Aerobic Exercise Suppresses Cancer Growth by Regulating Skeletal Muscle-Derived Oncogenes and Tumor Suppressors
상세 내용: The study examines how high-intensity aerobic exercise suppresses cancer growth by regulating skeletal muscle-derived oncogenes and tumor suppressors. It explores the molecular mechanisms behind exercise's anticancer effects using a colorectal cancer mouse model. VISQUE InVivo Smart was used to monitor cancer cell metastasis in mice injected with colorectal cancer cells, enabling the tracking of cancer progression during the study. The in vivo imaging revealed significant tumor growth reduction in mice subjected to high-intensity aerobic exercise, confirming exercise's inhibitory effect on metastasis.
학술지: Front Mol Biosci. 2022 Jun 21;9:818470.

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Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway 2022 in vivo System

제목: Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway
상세 내용: "The main goal of the paper is to investigate whether metformin can counteract the senescence of adipose-derived stem cells (ADSCs) and improve outcomes in osteoarthritis (OA) by reducing inflammation and cartilage degradation.
The Vieworks VISQUE in vivo imaging system was used to track the labeled ADSCs injected into the mice to assess their survival and localization in the knee joints at different time points (1, 3, and 7 days post-injection)(Oxidative Medicine and …). The imaging results showed that the fluorescence signal from the DiR-labeled ADSCs persisted up to 7 days, indicating that the cells survived and were active within the joint area during this period(Oxidative Medicine and …)."
학술지: Oxid Med Cell Longev. 2022 Jun 3;2022:4620254.

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Therapeutic strategies targeting uPAR potentiate anti–PD-1 efficacy in diffuse-type gastric cancer 2022 in vivo System

제목: Therapeutic strategies targeting uPAR potentiate anti–PD-1 efficacy in diffuse-type gastric cancer
상세 내용: This study investigates the potential of targeting the urokinase-type plasminogen activator receptor (uPAR) as a therapeutic approach to enhance anti-PD-1 efficacy in treating diffuse-type gastric cancer (DGC). Smart-LF was utilized to perform bioluminescent imaging on mouse models, allowing researchers to monitor tumor growth and evaluate the effects of the uPAR-targeted therapy. The imaging demonstrated that the combination of anti-uPAR and anti-PD-1 therapies significantly suppressed tumor growth and improved survival rates in the treated mouse models.
학술지: Sci Adv. 2022 May 25;8(21):eabn3774.

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Quantitative Biodistribution and Pharmacokinetics Study of GMP-Grade Exosomes Labeled with 89Zr Radioisotope in Mice and Rats 2022 in vivo System

제목: Quantitative Biodistribution and Pharmacokinetics Study of GMP-Grade Exosomes Labeled with 89Zr Radioisotope in Mice and Rats
상세 내용: "The study aimed to assess the quantitative biodistribution and pharmacokinetics of GMP-grade therapeutic exosomes labeled with zirconium-89 (89 Zr) after systemic intravenous administration in mice and rats.
The VISQUE InVivo Smart-LF imaging system was used for in vivo fluorescence imaging to track the distribution of DR-labeled exosomes in mice. The imaging results indicated that the exosomes were mainly distributed in the liver, with significant accumulation also observed in the kidney, lung, and spleen."
학술지: Pharmaceutics. 2022 May 24;14(6):1118.

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FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis 2022 in vivo System

제목: FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis
상세 내용: The study investigates the impact of FGF-2 signaling on nasopharyngeal carcinoma (NPC) metastasis, focusing on its role in promoting pericyte-macrophage crosstalk to drive tumor spread. VISQUE Invivo Elite was utilized for imaging GFP+ metastatic nodules in a mouse model to assess tumor spread following FGF-2 manipulation. Imaging revealed increased metastatic nodules in FGF-2–overexpressing tumors, suggesting that FGF-2 amplifies tumor metastasis by facilitating pericyte-driven macrophage recruitment
학술지: JCI Insight. 2022 May 23;7(10):e157874.

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Fluorescence-Shadowing Nanoparticle Clusters for Real-Time Monitoring of Tumor Progression 2022 in vivo System

제목: Fluorescence-Shadowing Nanoparticle Clusters for Real-Time Monitoring of Tumor Progression
상세 내용: "The study aims to develop a real-time tumor monitoring system using MMP-responsive gold nanoparticle (AuNP) clusters and quantum dots (QD) to detect tumor microenvironments, facilitating better therapeutic strategies.
The VISQUE InVivo Smart-LF system was utilized to visualize fluorescence changes in vivo, confirming the FRET-based quenching of QDs when the AuNPs and QDs formed clusters in response to MMP activity at tumor sites. The imaging results demonstrated significant fluorescence attenuation at tumor sites, which correlated with tumor size and enzyme levels, validating the system’s potential for tumor progression monitoring"
학술지: Biomacromolecules. 2022 Apr 22;23(8):3130–3141.

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Immunotherapeutic effects of recombinant Bacillus Calmette–Guérin containing sic gene in ex vivo and in vivo bladder cancer models 2022 in vivo System

제목: Immunotherapeutic effects of recombinant Bacillus Calmette–Guérin containing sic gene in ex vivo and in vivo bladder cancer models
상세 내용: The study aims to assess the efficacy of a recombinant Bacillus Calmette-Guérin (rBCG-sic) with enhanced immunotherapeutic effects on bladder cancer, targeting improved response rates in non-muscle invasive bladder cancer. VISQUE In Vivo Smart-LF was utilized to perform bioluminescence imaging to monitor bladder cancer progression in an orthotopic mouse model after rBCG-sic treatment. Imaging results showed that rBCG-sic significantly reduced tumor volume and slowed cancer progression compared to standard BCG, supporting its potential for greater anticancer efficacy.
학술지: Investig Clin Urol. 2022 Feb 23;63(2):228–237.

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A micellized bone morphogenetic protein-7 prodrug ameliorates liver fibrosis by suppressing transforming growth factor-β signaling 2022 in vivo System

제목: A micellized bone morphogenetic protein-7 prodrug ameliorates liver fibrosis by suppressing transforming growth factor-β signaling
상세 내용: "The main goal of the paper is to investigate the ability of a micellized bone morphogenetic protein-7 (BMP-7) prodrug to ameliorate liver fibrosis by suppressing transforming growth factor-β (TGF-β) signaling.
The Vieworks VISQUE in vivo Smart imaging system was used to acquire fluorescence images of mice administered with indocyanine green-labeled mPTD-BMP-7 to analyze its distribution in the liver. The imaging results showed that mPTD-BMP-7 was mainly delivered to the liver, indicating efficient hepatic delivery."
학술지: Am J Cancer Res. 2022 Feb 15;12(2):763–778.

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A novel dual-labeled small peptide as a multimodal imaging agent for targeting wild-type EGFR in tumors 2022 in vivo System

제목: A novel dual-labeled small peptide as a multimodal imaging agent for targeting wild-type EGFR in tumors
상세 내용: The study aims to develop and evaluate a dual-labeled imaging agent, Tc-99m SYPIPDT-ECG-TAMRA, for targeted imaging of tumors that overexpress wild-type epidermal growth factor receptor (wtEGFR). VISQUE In Vivo Smart-LF was employed for ex vivo fluorescence imaging to assess TAMRA-labeled agent uptake in excised tumor and organ tissues from mice. Imaging confirmed higher uptake of the agent in wtEGFR-positive tumors compared to wtEGFR-negative ones, demonstrating the agent's specificity for wtEGFR-positive cells.
학술지: PLoS One. 2022 Feb 4;17(2):e0263474

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A salicylaldehyde benzoyl hydrazone based near-infrared probe for copper(ii) and its bioimaging applications 2022 in vivo System

제목: A salicylaldehyde benzoyl hydrazone based near-infrared probe for copper(ii) and its bioimaging applications
상세 내용: This study develops a near-infrared (NIR) fluorescent probe, CySBH, for selective detection and imaging of copper (Cu²⁺) ions, aiming to aid in diagnosing copper-related diseases. VISQUE In Vivo Elite was employed for in vivo imaging to track the probe’s fluorescence response to Cu²⁺ in mice, visualizing its distribution and quenching in real-time. Imaging showed that the probe exhibited strong NIR fluorescence, which decreased with increased Cu²⁺ concentration, confirming the probe’s effective and selective response to Cu²⁺ ions in living subjects.
학술지: RSC Adv. 2022 Jan 24;12(5):3073–3080.

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Korean Red Ginseng Enhances Immunotherapeutic Effects of NK Cells via Eosinophils in Metastatic Liver Cancer Model 2022 in vivo System

제목: Korean Red Ginseng Enhances Immunotherapeutic Effects of NK Cells via Eosinophils in Metastatic Liver Cancer Model
상세 내용: This study explores how Korean Red Ginseng (KRG) combined with natural killer (NK) cell therapy enhances the immune response to suppress metastasis in a metastatic liver cancer model. VISQUE In Vivo Elite was used for bioluminescent imaging to track metastasis progression in vivo within the metastatic liver cancer model. The imaging revealed that the combination of KRG and NK cells significantly inhibited metastatic progression, reducing bioluminescence intensity compared to other treatment groups, thereby indicating an enhanced anti-cancer effect.
학술지: Nutrients. 2021 Dec 28;14(1):134.

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Real-Time Longitudinal Evaluation of Tumor Blood Vessels Using a Compact Preclinical Fluorescence Imaging System 2021 in vivo System

제목: Real-Time Longitudinal Evaluation of Tumor Blood Vessels Using a Compact Preclinical Fluorescence Imaging System
상세 내용: This study aims to use a compact preclinical fluorescence imaging system to monitor tumor blood vessel dynamics over time, focusing on evaluating the effects of angiogenesis inhibitors in real-time. VISQUE In Vivo Smart-LF was used for time-lapse imaging of tumor vasculature in mice injected with indocyanine green (ICG), allowing for detailed visualization of blood flow and vessel volume changes as the tumor progressed. The imaging revealed that tumor blood flow and vessel volume increased with tumor growth but decreased following angiogenesis inhibitor treatment, demonstrating the system’s utility for monitoring vascular responses to therapy.
학술지: Biosensors (Basel). 2021 Nov 23;11(12):471

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Local drug delivery using poly(lactic-co-glycolic acid) nanoparticles in thermosensitive gels for inner ear disease treatment 2021 in vivo System

제목: Local drug delivery using poly(lactic-co-glycolic acid) nanoparticles in thermosensitive gels for inner ear disease treatment
상세 내용: This study aims to develop a localized drug delivery system using poly(lactic-co-glycolic acid) (PLGA) nanoparticles in a thermosensitive gel for treating inner ear diseases, ensuring sustained release of dexamethasone (DEX) directly to the inner ear. VISQUE In Vivo Smart was utilized for in vivo fluorescence imaging to observe the biodistribution and retention of the coumarin-6-loaded PLGA nanoparticles in the inner ear after administration. Imaging revealed that the PLGA nanoparticle-gel formulation remained longer in the middle ear, allowing for a sustained release to the inner ear, as evidenced by prolonged fluorescence intensity up to 48 hours post-administration.
학술지: Drug Deliv. 2021 Oct 20;28(1):2268–2277

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BEX1 and BEX4 Induce GBM Progression through Regulation of Actin Polymerization and Activation of YAP/TAZ Signaling 2021 in vivo System

제목: BEX1 and BEX4 Induce GBM Progression through Regulation of Actin Polymerization and Activation of YAP/TAZ Signaling
상세 내용: This study investigates how BEX1 and BEX4 proteins influence glioblastoma (GBM) progression by promoting actin polymerization and activating YAP/TAZ signaling, especially following radiotherapy. VISQUE In Vivo Smart-LF was utilized for bioluminescent imaging to monitor tumor growth and progression in GBM-bearing mouse models following treatments. Imaging demonstrated significant tumor growth inhibition when radiotherapy was combined with Latrunculin B, an actin polymerization inhibitor, indicating potential suppression of GBM aggressiveness.
학술지: Int J Mol Sci. 2021 Sep 11;22(18):9845

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Downregulated CLIP3 induces radioresistance by enhancing stemness and glycolytic flux in glioblastoma 2021 in vivo System

제목: Downregulated CLIP3 induces radioresistance by enhancing stemness and glycolytic flux in glioblastoma
상세 내용: The paper aims to explore how downregulation of the protein CLIP3 contributes to radioresistance in glioblastoma by increasing stemness and glycolytic activity, and it investigates the potential of glimepiride, a diabetes drug, as a radiosensitizer to counteract this effect. Smart-LF was used to monitor tumor growth in mice models with orthotopic glioblastoma xenografts after treatment with radiation and glimepiride. The imaging revealed significant inhibition of tumor growth in glioblastoma models treated with a combination of glimepiride and radiation, suggesting an enhanced therapeutic effect compared to radiation alone.
학술지: J Exp Clin Cancer Res. 2021 Sep 6;40:282.

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Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen 2021 in vivo System

제목: Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen
상세 내용: The study aims to develop an adjuvant formulation combining DOTAP, CpG D35, and aluminum salt to enhance both T cell and antibody responses to protein antigens, thereby improving vaccine efficacy for infections and cancer. VISQUE in vivo Smart-LF was used to track antigen distribution in vivo, particularly focusing on how the adjuvant formulation influences antigen retention at the injection site and migration to lymph nodes. The system showed that the DOTAP-containing formulation retained antigens at the injection site and increased antigen presence in draining lymph nodes over time, suggesting improved delivery and immune response initiation.
학술지: PLoS One. 2021 Aug 2;16(8):e0254628

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CKAP4 Antibody-Conjugated Si Quantum Dot Micelles for Targeted Imaging of Lung Cancer 2021 in vivo System

제목: CKAP4 Antibody-Conjugated Si Quantum Dot Micelles for Targeted Imaging of Lung Cancer
상세 내용: The paper investigates the use of CKAP4 antibody-conjugated silicon quantum dot micelles (Si QD micelles-CKAP4) as a targeted imaging agent for lung cancer, aiming to improve fluorescence-guided surgery. Smart-LF was utilized for in vivo fluorescence imaging to track the biodistribution and targeting efficacy of Si QD micelles-CKAP4 in tumor-bearing mice. The imaging results showed strong fluorescence in lung cancer tissues compared to healthy tissues, confirming the Si QD micelles-CKAP4’s potential for specific targeting in lung cancer imaging.
학술지: Nanoscale Res Lett. 2021 Jul 31;16:124.

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Enzyme-Loaded pH-Sensitive Photothermal Hydrogels for Mild-temperature-mediated Combinational Cancer Therapy 2021 in vivo System

제목: Enzyme-Loaded pH-Sensitive Photothermal Hydrogels for Mild-temperature-mediated Combinational Cancer Therapy
상세 내용: The study aims to create a glucose oxidase-loaded, pH-sensitive photothermal hydrogel that can combine mild-temperature photothermal therapy (PTT) and starvation therapy to treat solid tumors effectively and safely. VISQUE in vivo Smart-LF was utilized to capture bioluminescence images, assessing the anti-metastasis effects of the hydrogels by measuring bioluminescence intensity in lung tissues. Imaging with the system revealed significantly reduced lung bioluminescence signals in mice treated with the hydrogel under laser irradiation, indicating effective inhibition of tumor metastasis to the lungs.
학술지: Front Chem. 2021 Jul 29;9:736468.

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Artificially engineered antiferromagnetic nanoprobes for ultra-sensitive histopathological level magnetic resonance imaging 2021 in vivo System

제목: Artificially engineered antiferromagnetic nanoprobes for ultra-sensitive histopathological level magnetic resonance imaging
상세 내용: The study aims to develop ultra-sensitive antiferromagnetic nanoparticle probes for high-resolution, non-invasive magnetic resonance imaging (MRI) at the histopathological level, with applications for detecting microscopic tumors and micrometastases. VISQUE InVivo Elite was used to capture fluorescence images of liver tissues in mice post-MRI to validate tumor targeting and distribution of the nanoprobe. Imaging with the VISQUE system confirmed specific targeting and strong fluorescence signals within microscopic tumors, corroborating the MRI findings and illustrating the probes' effectiveness in detecting small-scale tumors.
학술지: Nat Commun. 2021 Jun 22;12:3840

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Marriage of Virus‐Mimic Surface Topology and Microbubble‐Assisted Ultrasound for Enhanced Intratumor Accumulation and Improved Cancer Theranostics 2021 in vivo System

제목: Marriage of Virus‐Mimic Surface Topology and Microbubble‐Assisted Ultrasound for Enhanced Intratumor Accumulation and Improved Cancer Theranostics
상세 내용: The study aims to improve intratumor accumulation and therapeutic efficacy of nanoparticles for cancer treatment by combining virus-mimic surface topology with microbubble-assisted ultrasound. VISQUE in vivo Elite was used to conduct in vivo fluorescence imaging, selecting the ICG channel to assess nanoparticle accumulation in the tumor. The system successfully detected enhanced fluorescence intensity in tumors after treatment, demonstrating increased intratumor accumulation due to the combined nanoparticle design and ultrasound strategy.
학술지: Adv Sci (Weinh). 2021 May 14;8(13):2004670.

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In Vitro Photodynamic Effects of the Inclusion Nanocomplexes of Glucan and Chlorin e6 on Atherogenic Foam Cells 2021 in vivo System

제목: In Vitro Photodynamic Effects of the Inclusion Nanocomplexes of Glucan and Chlorin e6 on Atherogenic Foam Cells
상세 내용: The study investigated the in vitro photodynamic effects of Glucan/Chlorin e6 (Glu/Ce6) nanocomplexes on atherogenic foam cells as a potential treatment for atherosclerosis. The researchers used VISQUE in vivo Smart-LF to capture fluorescence images of the Glu/Ce6 nanocomplexes and their singlet oxygen generation under laser irradiation. The imaging showed successful internalization of the Glu/Ce6 nanocomplexes in foam cells, confirming efficient delivery of the photosensitizer Ce6 to targeted cells and demonstrating high singlet oxygen generation upon laser exposure.
학술지: Int J Mol Sci. 2020 Dec 26;22(1):177

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Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer 2021 인비보이미징시스템

제목: Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer
상세 내용: Trastuzumab은 HER2 유래 위암 치료의 주 요소이나, 충분치 않은 세포 반응과 약물 저항으로 환자의 반응성은 낮습니다. 이 논문에서는 HER2의 후속 경로인 HER2-SHCBP1-PLK1 axis의 과잉 활성화를 통해 HER2가 세포 분열을 촉진하여 종양 생성에 관여하고 trastuzumab 치료에 대한 낮은 반응성과 관련있는 것을 보고하였습니다. SHCBP1-PLK1 상호작용의 억제자인 Theaflavine-3, 3’-digallate (TFBG)가 낮은 Trastuzumab 민감성을 높여, Trastuzumab 과 같이 처리할 경우 HER2 양성 위암의 성장을 억제하는 데에 매우 효과적이라는 것을 확인하였습니다. NCI-N87/luc tumor xenograft 모델 마우스에 Trastuzumab을 처리하면서 SHCBP1를 knockdown하거나 TFBG를 같이 처리하고 Visque InVivo Smart-LF로 in vivo 발광 촬영하여 시간에 따른 암 조직의 변화를 확인하였습니다.
학술지: Nature Communications 2021 May 14;12(1):2812

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Lung-selective 25-hydroxycholesterol nanotherapeutics as a suppressor of COVID-19-associated cytokine storm 2021 인비보이미징시스템

제목: Lung-selective 25-hydroxycholesterol nanotherapeutics as a suppressor of COVID-19-associated cytokine storm
상세 내용: Cytokine storm이라 불리는 cytokine 수준의 증가와 25-hydroxycholesterol (25-HC)의 감소는 급성호흡곤란증후군(acute respiratory distress syndrome, ARDS)과 패혈증을 일으키며 COVID-19의 발병 과정에 중요한 역할을 수행하는 것으로 알려졌습니다. 이 논문에서는 세포내 콜레스테롤의 회복과 cytokine storm의 억제를 위한 약물 후보물질로, 25-HC과 didodecyldimethylammonium bromide (DDAB)을 합친 nanovesicles (25-HC@DDAB)을 개발하였습니다. 25-HC@DDAB는 폐 조직에 선택적으로 축적되고, 환자 유래 peripheral blood mononuclear cells (PBMCs) 실험에서 염증성 cytokine 수준을 감소시켰습니다. 마우스에 25-HC@DDAB를 꼬리 혈관 주사로 주입한 후, 특정 시간에 Visque InVivo Smart로 ex vivo 형광 촬영하여 시간에 따른 체내 분포를 관찰하여, 폐에서의 축적을 확인하였습니다.
학술지: Nano Today 2021 Jun; 38: 101149 2021 Jan 11;

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RNA interference-mediated suppression of TNF-α converting enzyme as an alternative anti-TNF-α therapy for rheumatoid arthritis 2021 인비보이미징시스템

제목: RNA interference-mediated suppression of TNF-α converting enzyme as an alternative anti-TNF-α therapy for rheumatoid arthritis
상세 내용: 류머티즘성 관절염 환자에게는 tumor necrosis factor-α (TNF-α)가 과량 발현되어, 치료시 항TNF-α 치료법을 사용합니다. 이 논문에서는 TNF-α의 수준을 낮출 수 있는 새로운 방법으로, TNF-α 전환 효소 (TACE)를 억제하는 방법을 제시하였습니다. 이를 위해, TACE 대상 shRNA(shTACE)와 질병 조직 표적 peptide carrier로 질병 조직 표적 RNA interference system을 제작하였습니다. shTACE/peptide carrier complex는 collagen induced arthritis (CIA) 모델 동물 실험에서 항 염증 효과와 항 파골세포 효과를 보이면서, 관절염 증상을 완화하였습니다. 또한, 환자로부터 유래한 인간 유래 세포 실험에서도 유사한 결과를 보였습니다. CIA 모델 동물에 shTACE/peptide carrier complex를 혈관 주사한 후, Visque InVivo Smart로 형광 촬영하여 shTACE/peptide carrier complex의 체내 분포를 확인하였습니다.
학술지: Journal of Controlled Release 2021 Feb 10;330:1300-1312

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Fenton-like reaction, glutathione reduction, and photothermal ablation-built-in hydrogels crosslinked by cupric sulfate for loco-regional cancer therapy 2021 인비보이미징시스템

제목: Fenton-like reaction, glutathione reduction, and photothermal ablation-built-in hydrogels crosslinked by cupric sulfate for loco-regional cancer therapy
상세 내용: 본 논문에서는 Fenton 유사 반응 연관 chemodynamic therapy (CDT)와 고열 유발 photothermal therapy (PTT)를 결합하여 암의 국소 치료에 적용하기 위해, crosslinked hydrogel system 방법으로 유방암에 적용할 수 있는 HC/Cu/ICG hydrogel을 개발하였습니다. Cupric sulfate (Cu), catechol-functionalized hyaluronic acid (HC), indocyanine green (ICG)를 결합하여 만든 HC/Cu/ICG hydrogel은 in vivo 실험에서 심각한 독성을 보이지 않으면서도 유방암에 대한 국소 CDT, PTT 치료 효과를 보였습니다. 연구자들은 마우스의 피하에 HC/Cu/ICG hydrogel을 주입한 후, 특정 날짜에 Visque InVivo Smart로 in vivo 형광 촬영하고 정량하여 체내에서의 생분해성을 확인하였습니다.
학술지: Biomaterials Science 2021 Feb 7;9(3):847-860

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Dissecting the impact of target-binding kinetics of protein binders on tumor localization 2021 인비보이미징시스템

제목: Dissecting the impact of target-binding kinetics of protein binders on tumor localization
상세 내용: 단백질 기반의 치료법의 치료 효과를 향상시키기 위해서는 체내에서의 작용을 체계적으로 제어할 수 있어야 합니다. 이 논문에서는 연구자들은 EGFR에 다른 결합 친화성을 가진 리피바디(repebody)를 종양 모델 마우스에 사용하여 in vivo 상태에서의 암 조직에 대한 단백질의 결합 친화성의 영향을 연구하고, 최적의 친화성을 가지면 결합 부위 장애물 효과를 극복하여 암 조직에 대한 단백질의 침투 및 축적을 촉진할 수 있다는 것을 보였습니다. A431 종양 모델 마우스에 다양한 리피바디를 혈관 주사한 후, 특정 시간에 Visque InVivo Smart-LF로 in vivo 형광 촬영하여 리피바디의 전신 체내 분포를 확인하고, 정량화하였습니다.
학술지: iScience 2021 Feb 19; 24(2): 102104.

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Antibreast Cancer Activity of Aspirin-Conjugated Chalcone Polymeric Micelles 2021 인비보이미징시스템

제목: Antibreast Cancer Activity of Aspirin-Conjugated Chalcone Polymeric Micelles
상세 내용: 본 논문에서, 연구자들은 아스피린을 chalcone과 접합한 형태의 새로운 nanoparticle (AS-DK143-loaded NP)을 개발하였습니다. AS-DK143 NP은 in vitro 실험에서 배양한 4T1 cell의 생존성을 감소시켰고, in vivo 실험에서 암세포에 대한 선택적 표적능을 보이고, 암 조직의 크기를 현저히 줄여, 충분한 항암 효과를 보였습니다. 4T1 cell을 피하 이종 이식한 마우스에 AS-DK143 NP을 주입한 후, 특정 날짜에 장기를 적출하여 Visque InVivo Smart로 ex vivo 형광 촬영하여 AS-DK143 NP의 체내 분포를 확인하였습니다.
학술지: Macromolecular Research 2021 Jan 28;29:105–110

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Meso-pyridinium substituted BODIPY dyes as mitochondria-targeted probes for the detection of cysteine in living cells and in vivo 2021 인비보이미징시스템

제목: Meso-pyridinium substituted BODIPY dyes as mitochondria-targeted probes for the detection of cysteine in living cells and in vivo
상세 내용: Cysteine (Cys)은 미토콘드리아에 산화 손상을 일으키는 ROS에 대한 항산화제로, 많은 생리, 병리 과정에서 중요한 역할을 수행하는 bio-thiol입니다. 본 논문에서는 BODIPY에서 일부분을 치환하여, Cys를 구분할 수 있는 형광 probe인 BDP-S-o-Py+를 개발하였습니다. BDP-S-o-Py+는 미토콘드리아를 타겟으로 하는 probe로, Cys를 감지할 수 있습니다. in vitro 및 in vivo 실험으로 확인한 결과, BDP-S-o-Py+로 살아있는 세포 내의 미토콘드리아 Cys를 영상 촬영할 수 있으며, in vivo에서도 내생적인 Cys를 관찰할 수 있었습니다. 피하에 Cys를 주입한 마우스에 BDP-S-o-Py+를 주입한 후, Visque InVivo Smart로 in vivo 형광 촬영하여 BDP-S-o-Py+의 체내 Cys 감지 능력을 확인하였습니다.
학술지: Dyes and Pigments 2021 Mar;187:109089

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Characterization of adipose-derived stromal/stem cell spheroids versus single-cell suspension in cell survival and arrest of osteoarthritis progression 2021 인비보이미징시스템

제목: Characterization of adipose-derived stromal/stem cell spheroids versus single-cell suspension in cell survival and arrest of osteoarthritis progression
상세 내용: 본 논문에서, 연구자들은 osteoarthritis (OA)의 랫 실험에서 지방 유래 줄기 세포 (adipose-derived stromal/stem cells, ASCs)의 spheroid가 이전에 사용하던 single cell보다 세포의 생존율이 높고, surgically induced osteoarthritis (OA)의 진행을 억제한다는 것을 밝혔습니다. 또한 microwell을 사용한 ASCs spheroid 제작의 최적 조건을 실험하였습니다. 수술로 osteoarthritis (OA)를 유발한 랫의 무릎 관절에 ASCs Spheroid와 singel cell을 각각 주입한 후, Visque InVivo Smart로 형광 in vivo 촬영하여 체내에서의 유지 시간을 확인하여 ASC spheroid가 single cell을 사용하는 경우보다 좀더 오래 유지됨을 확인였습니다.
학술지: Journal of Biomedical Materials Research Part A 2021 Jun;109(6):869-878

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Optically activatable photosynthetic bacteria-based highly tumor specific immunotheranostics 2020 인비보이미징시스템

제목: Optically activatable photosynthetic bacteria-based highly tumor specific immunotheranostics
상세 내용: 이 논문의 저자들은 박테리아 항암 치료를 위한 박테리아로, purple photosynthetic bacteria (PPSB)를 제안하였습니다. PPSB는 비병원성으로, 강한 NIR I/II 형광, 강한 광열 변환, 뛰어난 활성산소종 생성, 대조적인 광음향 효과 등을 가져, 종양에 대한 높은 표적 제거 능력과 정확한 종양 표시 능력을 보였습니다. 더물어, 단기 단식은 박테리아 치료의 효율성을 증가시켰습니다. 종양을 생성한 마우스에 PPSB를 혈관 주사한 후, 특정 시간에 Visque InVivo Smart-LF로 in vivo, ex vivo 형광 촬영하고 정량하여, PPSB의 체내 분포와 암 표적능을 확인하였습니다.
학술지: Nano Today 2020 Apr;37:101100

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Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta–Mediated Epithelial–Mesenchymal Transition 2020 인비보이미징시스템

제목: Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta–Mediated Epithelial–Mesenchymal Transition
상세 내용: 본 논문에서는 신장 섬유증의 효과적인 치료를 위해 섬유증의 진행을 억제하는 bone morphogenetic protein-7 (BMP-7)을 기반으로 micelle 형태의 protein transduction domain (PTD) 융합 BMP-7 (mPTD-BMP-7)을 개발하였습니다. mPTD-BMP-7은 in vitro와 in vivo 편측 요관 폐쇄 모델 실험에서 transforming growth factor-beta (TGF-β) 매개 상피-중간엽 전이 과정을 성공적으로 억제하였습니다. 또한, 신동맥을 통한 mPTD-BMP-7의 동맥 내 투여 방법을 개발하여 돼지 동물 실험에서 임상적 타당성을 확인였습니다. 돼지 동물실험에서, 신동맥을 통해 투여된 mPTD-BMP-7는 효과적으로 Bowman 공간으로 전달되어 편측 요관 폐쇄로 인한 신장 섬유증을 억제하였습니다. 돼지의 신동맥에 mPTD-BMP-7을 투여한 후, 특정 시간에 신장을 적출하여 Visque InVivo Smart로 형광 ex vivo 촬영하고, 정량하여 mPTD-BMP-7의 신장 내 분포를 확인하였습니다.
학술지: Frontiers in Pharmacology 2020 Nov 19;11:591275

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Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer 2020 인비보이미징시스템

제목: Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer
상세 내용: 본 논문에서는 chemotherapy와 photothermal therapy (PTT)를 동시에 할 수 있는 biophotonic nanoplatform으로, PTT-photosensitizer인 zirconium carbide (ZrC) nanosheet와 항암 전구 약물인 SN38-Nif을 결합, 개발하였습니다. 이렇게 개발된 ZrC@prodrug biophotonic nanoplatform을 in vitro와 in vivo 실험으로 테스트한 결과, 강력한 항암 효과를 보였습니다. 특히, 마우스 모델 실험에서 ZrC@prodrug은 암의 재발과 전이, 염증, 혈관 생성을 현저하게 억제했습니다. 연구자들은 ZrC@prodrug과 비교군을 마우스 모델에 처리한 후, Visque InVivo Elite로 2주 후 간의 종양을 ex vivo 형광 촬영하고 정량하여 ZrC@prodrug의 종양 억제 효과를 확인하였습니다.
학술지: Advanced Science 2020 Nov 5;7(24):2001191

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Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis 2020 인비보이미징시스템

제목: Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis
상세 내용: bone morphogenetic protein-7 (BMP-7)는 많은 섬유증 질병을 회복시키는 치료용 성장 인자로, 복막 투석(peritoneal dialysis, PD)으로 발생하는 복막 섬유증(peritoneal fibrosis)에도 적용할 수 있다. 본 논문에서 연구자들은 protein transduction domains (PTD)을 사용한 새로운 약물 전달 체계를 개발하여 BMP-7에 적용하였습니다. 이렇게 개발된 PTD-BMP-7을 epithelial-mesenchymal transition (EMT) 관련 섬유증에 적용하여 치료 효과를 검증하였습니다. 랫을 활용한 in vivo 실험에서 PTD-BMP-7의 복강 내 주입은 복막 투석으로 발생한 변화를 현저히 줄이고, 이미 유발된 복막투석 섬유증의 진행을 매우 억제하였습니다. 연구자들은 PTD-BMP-7을 마우스에 복강 내 주입한 후 특정 시간에 Visque InVivo Elite로 in vivo 형광 촬영, Visque InVivo Smart로 ex vivo 형광 촬영하여 체내 분포 및 체내 반감기를 확인하였습니다.
학술지: Journal of Cellular and Molecular Medicine 2020 Nov;24(22):13507-13522

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“Star” miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer 2020 인비보이미징시스템

제목: “Star” miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer
상세 내용: 본 논문에서는 연구자들은 종양 세포의 전이를 막기 위해 DPC/miR-34a란 nanoplex를 만들었습니다. DPC/miR-34a는 CM 말단을 갖고 있는 Dextrin 조작 1.8 k PEI (Dextrin-PEI-CM nanoplex (DPC))에 cell adhesion molecules인 CD44의 억제제인 Star miR-34a을 붙여 만들었습니다. DPC/miR-34a nanoplex는 in vitro cell invasion assay와 in vivo 암 전이 모델에서 암세포의 침입을 억제하였습니다. 또한, 폐암 전이 모델과 동위 MDA-MB-231 종양 모델에서 뛰어난 항암 효과와 항전이 효과를 보였습니다. 동위 MDA-MB-231 종양 모델 마우스에 DPC/miR-34a을 혈관 주입한 후, 정해진 시간에 Visque InVivo Elite로 in vivo 형광 촬영하여 주입 후 시간에 따른 체내 분포를 확인하였습니다.
학술지: Journal of Controlled Release 2020 Oct 10;326:615-627

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Ferritin Nanocage-Based Methyltransferase SETD6 for COVID-19 Therapy 2020 인비보이미징시스템

제목: Ferritin Nanocage-Based Methyltransferase SETD6 for COVID-19 Therapy
상세 내용: 본 논문에서는 ferritin nanocage 기반의 운반체에 CPP(cell-penetrating peptide)인 TAT-NBD와 SET-domain containing 6 (SETD6)를 결합하여 TAT NBD-short ferritin-SETD6 (TFS)를 만들었습니다. TFS는 SETD6의 수준을 향상시켜 바이러스 감염 매개 염증을 매개하는 nuclear factor-κB (NF-κB) signaling을 감소시켰습니다. cytokine storm 모델 마우스에 TFS를 혈관 주사한 결과, 생존률이 회복되고 폐 조직의 손상과 cytokine 수준이 줄어들었습니다. 마우스에 TFS를 혈관 주사한 후, 특정 시간에 장기를 적출하여 Visque InVivo Smart로 형광 ex vivo 촬영하여 TFS의 체내 분포를 확인하였습니다.
학술지: Advanced Functional Materials 2020 Nov 25;30(48):2006110

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A brain tumor-homing tetra-peptide delivers a nano-therapeutic for more effective treatment of a mouse model of glioblastoma 2020 인비보이미징시스템

제목: A brain tumor-homing tetra-peptide delivers a nano-therapeutic for more effective treatment of a mouse model of glioblastoma
상세 내용: CPP(Organ-specific cell-penetrating peptides)는 체내에서 치료 물질을 특정 조직에 운반하는데 매우 효과적인 물질로, 암 치료에 있어 주목받고 있습니다. 본 논문에서는 glioblastoma multiforme (GBM) brain tumor을 목적으로 하는 새로운 CPP로, amino acid sequence serine-isoleucine-tyrosine-valine(SIWV)를 개발하고, SIWV의 GBM에 대한 특이성을 in vitro와 in vivo 실험에서 확인하였습니다. SIWV의 체내 분포와 약물 운반 물질로서의 가능성은 항암제인 SN-38을 연결한 SIWV를 GBM xenograft 마우스에 주입한 후, Visque InVivo Smart-LF로 정해진 시간에 in vivo 및 ex vivo 촬영하고 정량하여 확인하였습니다.
학술지: Nanoscale Horizons 2020 Jul 27;5(8):1213-1225

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Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature 2020 인비보이미징시스템

제목: Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature
상세 내용: 본 논문에서는 두 종류의 Jet-lagged nanoparticles을 개발하여 vascular normalization therapy와 tumor cell metabolic treatment을 결합하였습니다. apatinib (APA)-loaded TEC-targeting nanodrug (APA/MCP)는 VEGF/VEGFR2를 차단하여 tumor endothelial cells (TECs)의 증식을 억제하였고, lonidamine (LND)-loaded tumor cell-targeting nanodrug (LND/MCA)은 Lactic acid (LA)의 방출을 억제하여 암의 산 대사 과정을 바꿨습니다. 여기에 더하여, programmed death 1 (PD-1) drug와 Jet-lagged nanoparticles을 같이 처리하는 경우의 치료 효과가 PD-1만 처리하는 경우보다 3배 더 높은 효과를 보였습니다. Jet-lagged nanoparticles을 종양이 생성된 마우스에 주입한 후, 정해진 시간마다 Visque InVivo Elite로 in vivo 형광 촬영하고 장기를 적출하여 ex vivo 형광 촬영하여 종양 표적능과 체내 분포 특성을 확인하고, 정량하였습니다.
학술지: Advanced Healthcare Materials 2020 Jun;9(12):e2000075

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Dual functional nanoparticles containing SOX duo and ANGPT4 shRNA for osteoarthritis treatment 2020 인비보이미징시스템

제목: Dual functional nanoparticles containing SOX duo and ANGPT4 shRNA for osteoarthritis treatment
상세 내용: 본 논문에서는 골관절염(osteoarthritis, OA)의 진행을 억제하고, 연골 형성 (chondrogenesis)을 촉진하기 위한 방법으로, dexamethasone-conjugated polyethylenimine (DEX PEI)와, SOX duo (SOX-9, -6)와 ANGPTL4 small hairpin RNA (shANG)를 가진 minicircle plasmid (MC)을 합쳐 nanoparticle [MC SOX9/6/shANG]을 제작하였습니다. 해당 nanoparticle로 처리한 지방 유래 줄기 세포 (Adipose-derived stem cells, ADSCs) (MC SOX9/6/shANG-tADSCs)의 in vitro, in vivo 실험에서, 줄기 세포의 연골 형성은 촉진되고, 골관절염의 감염은 억제되는 것을 확인하였습니다. 형광으로 표지한 MC SOX9/6/shANG-tADSCs 줄기 세포를 수술로 골관절염을 유발한 랫의 오른쪽 무릎 관절에 주입한 후, 정해진 시간에 Visque InVivo Elite로 촬영하여 체내 분포를 추적, 확인하였습니다.
학술지: Journal of Biomedical Materials Research Part B: Applied Biomaterials 2020 Jan;108(1):234-242

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Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery 2020 인비보이미징시스템

제목: Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery
상세 내용: 본 논문에서는 허혈성 뇌졸증의 신경학적, 기능적 회복을 위해 nanozyme을 활용한 새로운 치료법을 제안하였습니다. Nanozyme은 뛰어난 항염, 항 산화능을 가지고 있지만, 허혈성 뇌졸증의 병변 부위에서 축적이 충분히 되지 않아 치료 효과를 보이기 힘들었습니다. 연구자들은 nanozyme을 활용한 허혈성 뇌졸증에 대한 비침습적 활성 표적화 치료법을 개발하였습니다. 구체적으로, nanozyme을 호중구 유사 세포막으로 코팅하여 손상 부위로의 nanozyme 전달을 향상시켰습니다. 마우스에 transient middle cerebral artery occlusion (tMCAO)으로 ischemic stroke를 일으키고, FITC로 표지한 neutrophil-like cell membrane (NCM)-coated mesoporous Prussian blue nanozyme (MPBzyme@NCM)을 혈관 내 주사한 후, Visque InVivo Elite로 in vivo 형광 촬영하여 시간에 따른 병변 부위에서의 축적을 확인하였습니다.
학술지: ACS Nano

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Interrogation of Folic Acid-Functionalized Nanomedicines: The Regulatory Roles of Plasma Proteins Reexamined 2020 인비보이미징시스템

제목: Interrogation of Folic Acid-Functionalized Nanomedicines: The Regulatory Roles of Plasma Proteins Reexamined
상세 내용: 본 논문에서는 엽산 (folic acid, FA)를 사용하여 nanomedicine의 표적 전달이 향상되는 기전을 확인하였습니다. Nanomedicine을 실험할 때, 엽산을 사용하면 표적 전달이 촉진된다는 것은 알려져 있지만, 구체적인 기전은 아직 밝혀진 것이 없습니다. 엽산을 liposome의 표면에 달면 IgM의 흡수가 촉진되어 수용체 인식을 막고 보체 활성화가 가속되는 것을 in vivo 상에서 확인하였습니다. 그리고, nanoparticle에 적용하는 경우에도 IgM의 흡수에 취약했습니다. 종양을 생성한 마우스에 엽산을 표지한 liposome (FA-sLip)을 혈관 주사한 후, Visque InVivo Elite로 in vivo 형광 촬영하여, FA-sLip의 체내 분포를 확인하였습니다.
학술지: ACS Nano

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Supramolecular Container-Mediated Surface Engineering Approach for Regulating the Biological Targeting Effect of Nanoparticles 2020 인비보이미징시스템

제목: Supramolecular Container-Mediated Surface Engineering Approach for Regulating the Biological Targeting Effect of Nanoparticles
상세 내용: 본 논문의 연구자들은 nanoparticle의 표면에 ligand를 붙이는 방법으로, supramolecular container를 사용하는 것을 제시하였습니다. 여기에서는 Acyclic cucurbituril (aCB) molecular container를 사용하여 nanoparticle과 ligand를 화학적으로 결합하여 복합체를 이루게 하여, 물속에서의 분산성과 ligand의 표적능이 향상되는 것을 확인하였습니다. 특히, 향상된 표적능은 in vitro와 in vivo 실험에서 촬영하여 확인할 수 있었습니다. 종양을 생성한 마우스에 rhodamine B isothiocyanate (RITC)로 표지한 nanoparticle을 혈관 주사한 후, 정해진 시간에 Visque InVivo Elite로 형광 촬영하여 nanoparticle의 biodistribution을 관찰하여 표적능이 향상되었음을 확인하였습니다.
학술지: Nano Letters

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Enhancement of Nanozyme Permeation by Endovascular Interventional Treatment to Prevent Vascular Restenosis via Macrophage Polarization Modulation 2020 인비보이미징시스템

제목: Enhancement of Nanozyme Permeation by Endovascular Interventional Treatment to Prevent Vascular Restenosis via Macrophage Polarization Modulation
상세 내용: 본 논문에서는 혈관 내 중재 치료 후 발생하는 혈관 재협착을 줄이기 위한 방법으로, nanozyme을 활용한 방법을 제시하였습니다. Nanozyme은 혈관 재협착을 악화시키는 주요 요인인 reactive oxygen species (ROS)를 제거하나, 혈관 내피 세포를 효율적으로 통과하지 못합니다. 이 논문에서는 혈관 내 중재 치료시 발생하는 내피 세포 박리 현상을 통해 nanozyme인 Prussian blue nanozyme (PBzyme)을 혈관 내벽으로 침투시켜 ROS를 제거하여 혈관 재협착이 일어나지 못하도록 하였습니다. 연구자들은 Visque InVivo Elite로 마우스의 혈관을 in vivo 형광 촬영하여 PBzyme이 있는 경우 vascular balloon injury (VBI) 처리 시 혈관 재협착이 일어나는지 확인하였습니다.
학술지: Advanced Functional Materials

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A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues 2020 인비보이미징시스템

제목: A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues
상세 내용: 본 논문에서는 신경학적 질병과 강하게 연관된 Acetylcholinesterase (AChE)의 활동을 영상화할 수 있는 substrate를 개발하였습니다. 개발된 substrate인 P10은 butyrylcholinesterase (BChE)와 carboxylesterase (CE)으로부터의 간섭을 효과적으로 제거하면서 AChE의 활성을 촬영할 수 있음을 in vitro 실험으로 확인, 증명하였습니다. 그리고, 마우스 뇌에서의 AChE의 활성을 Visque InVivo Elite로 in vivo와 ex vivo 촬영을 하여 측정, 확인하였습니다.
학술지: Chemical Science

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Molecular Design of Conjugated Small Molecule Nanoparticles for Synergistically Enhanced PTT/PDT 2020 인비보이미징시스템

제목: Molecular Design of Conjugated Small Molecule Nanoparticles for Synergistically Enhanced PTT/PDT
상세 내용: Photothermal therapy (PTT)와 photodynamic therapy (PDT)의 동시 적용은 그 상승 효과 때문에 강화된 암 치료법으로 연구되고 있습니다. 본 논문에서는 PTT/PDT를 를 동시에 적용할 수 있는 IID-ThTPA nanoparticles (IID-ThTPA NPs)을 개발하여 in vivo와 in vitro에서 가능성을 실험하였습니다. IID-ThTPA nanoparticles는 isoindigo (IID)를 selective acceptor로, triphenylamine (TPA)를 donor로 하여 제작되었습니다. 이렇게 만들어진 IID-ThTPA NPs은 PTT/PDT 실험에서 높은 성능을 보였고, 종양 제거 효과도 매우 뛰어났습니다. 마우스에 IID-ThTPA NPs을 주입 후 정해진 시간에 장기를 적출하여 Visque InVivo Elite로 형광 촬영을 하여 체내 분포를 확인하였습니다.
학술지: Nano-Micro Letters

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In Vivo Imaging of Senescent Vascular Cells in Atherosclerotic Mice Using a β-Galactosidase-Activatable Nanoprobe 2020 인비보이미징시스템

제목: In Vivo Imaging of Senescent Vascular Cells in Atherosclerotic Mice Using a β-Galactosidase-Activatable Nanoprobe
상세 내용: 본 논문에서는 암 치료 방법으로 주목받고 있는 Photodynamic therapy (PDT)를 위한 근적외선 (NIR) 영역의 새로운 Photosensitizer (PS)로 Monoiodo Aza-BODIPY를 개발하여 in vivo 및 ex vivo 연구로 효과를 확인하였습니다.
BODIPY는 여러 특징으로, 이상적인 PS로 주목받고 있는 물질인데, 그 중 aza-BODIPY는 높은 광흡수율, NIR 파장(650 - 900nm) 흡수 등이 특징입니다. aza-BODIPY를 변형한 물질 중, Monoiodo Aza-BODIPY는 뛰어난 광안정성, 화학적 안정성, 광세포독성을 보였습니다. 그리고, in vivo 및 in vitro 연구를 통해 적은 투여량과 1회 광 조사에서도 우수한 항암 효능을 보이는 것을 확인하였습니다.
Visque InVivo Smart로 in vivo 및 ex vivo 상태에서 마우스의 장기를 촬영하여 주입 후 시간에 따른 Monoiodo Aza-BODIPY의 장기 분포를 확인하였습니다.
학술지: Analytical Chemistry

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Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy 2020 인비보이미징시스템

제목: Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy
상세 내용: 본 논문에서는 암 치료 방법으로 주목받고 있는 Photodynamic therapy (PDT)를 위한 근적외선 (NIR) 영역의 새로운 Photosensitizer (PS)로 Monoiodo Aza-BODIPY를 개발하여 in vivo 및 ex vivo 연구로 효과를 확인하였습니다.
BODIPY는 여러 특징으로, 이상적인 PS로 주목받고 있는 물질인데, 그 중 aza-BODIPY는 높은 광흡수율, NIR 파장(650 - 900nm) 흡수 등이 특징입니다. aza-BODIPY를 변형한 물질 중, Monoiodo Aza-BODIPY는 뛰어난 광안정성, 화학적 안정성, 광세포독성을 보였습니다. 그리고, in vivo 및 in vitro 연구를 통해 적은 투여량과 1회 광 조사에서도 우수한 항암 효능을 보이는 것을 확인하였습니다.
Visque InVivo Smart로 in vivo 및 ex vivo 상태에서 마우스의 장기를 촬영하여 주입 후 시간에 따른 Monoiodo Aza-BODIPY의 장기 분포를 확인하였습니다.
학술지: Journal of Medicinal Chemistry
2020 Sep 10;63(17):9950-9964

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Detecting Cysteine in Bioimaging with a Near‐Infrared Probe Based on a Novel Fluorescence Quenching Mechanism 2020 인비보이미징시스템

제목: Detecting Cysteine in Bioimaging with a Near‐Infrared Probe Based on a Novel Fluorescence Quenching Mechanism
상세 내용: 본 논문에서는 cysteine (Cys)을 감지할 수 있는 NIR turn-on fluorescent probe (BDP-NIR)를 개발, 소개하였습니다.
BDP-NIR은 BODIPY를 기반으로 구조를 일부 변경하여 만든 형광 probe로, Cys 존재 시 Stokes shift (105 nm)가 일어나 Cys를 감지할 수 있습니다. 특히, BDP-NIR은 0μM부터 500μM까지의 Cys 농도 범위에서 농도에 선형 비례하여 형광 밝기가 비례해서 Cys를 정량할 수 있었습니다. 그리고, Visque InVivo Elite로 마우스를 촬영하여 endogenous Cys도 감지할 수 있음을 확인하였습니다.
학술지: ChemBioChem

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Heme Oxygenase 1-Targeted Hybrid Nanoparticle for Chemo- and Immuno-Combination Therapy in Acute Myelogenous Leukemia 2020 인비보이미징시스템

제목: Heme Oxygenase 1-Targeted Hybrid Nanoparticle for Chemo- and Immuno-Combination Therapy in Acute Myelogenous Leukemia
상세 내용: 본 논문에서는 Acute myelogenous leukemia (AML, 급성 골수성 백혈병)에 대한 새로운 치료 방법으로, Hybrid Nanoparticle을 개발, human AML-bearing orthotopic mouse model에서 그 효과를 확인하였습니다.
화학 저항성 AML을 유발하는 효소인 Heme oxygenase 1의 반응을 억제하는 tin mesoporphyrin (SnMP)과 백혈병 세포 표적 항체를 lipid-polymer hybrid nanoparticle (hNP)에 실어 백혈병 세포의 화학 민감도를 강화하는 HO1-inhibiting T-hNP (T-hNP/SnMP)를 만들었습니다. 이렇게 만들어진 T-hNP/SnMP는 AML의 마우스 모델에서 화학치료제인 daunorubicin의 치료 효과를 향상시켰으며, 골수세포를 재프로그램하여 면역 반응을 증폭시켜, AML의 새로운 치료 방법으로의 가능성을 입증하였습니다.
Visque InVivo Smart로 마우스의 장기를 촬영하여 주입 후 시간에 따른 T-hNP/SnMP의 장기 분포를 확인하고, 골수에 분포하는 것을 확인하였습니다.
학술지: Advanced Science
2020 Jul; 7(13): 2000487

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Penta-fluorophenol: A Smiles rearrangement-inspired cysteine-selective fluorescent probe for imaging of human glioblastoma 2020 인비보이미징시스템

제목: Penta-fluorophenol: A Smiles rearrangement-inspired cysteine-selective fluorescent probe for imaging of human glioblastoma
상세 내용: 본 논문에서는 뇌종양의 일종인 glioblastoma (GBM)를 상대적으로 간단히 진단, 추적할 수 있는 새로운 biomarker, electron-deficient penta-fluorophenol (NPO-B)를 개발, 소개하였습니다. NPO-B는 GBM의 표지인 cysteine (Cys)을 감지하는 형광 probe로, 독성과 면역 반응을 보이지 않으면서 Cys의 양을 측정할 수 있습니다. 특히, NPO-B가 GBM을 잘 감지하는지 xenograft 마우스에서 적출한 뇌와 장기, 환자에게서 적출한 조직을 대상으로 Visque InVivo Elite로 ex vivo imaging을 하여 확인하였습니다.
학술지: Chemical Science
2020 May 11;11(22):5658-5668

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Selenium and dopamine-crosslinked hyaluronic acid hydrogel for chemophotothermal cancer therapy 2020 인비보이미징시스템

제목: Selenium and dopamine-crosslinked hyaluronic acid hydrogel for chemophotothermal cancer therapy
상세 내용: 본 논문에서는 암의 치료를 위해 selenuium (Se)과 dopamine (HD)을 indocyanine green (ICG)와 함께 hyaluronic acid (HA) hydrogel에 조합해 HD/Se/ICG gel을 개발하였습니다. 이렇게 만들어진 gel은 주사제로 사용이 용이하고, 생분해성이 낮아 오래 유지되며, 충분한 photothermal therapy 효율성을 보여, 심각한 독성 없이 매우 효율적인 종양 성장 억제능을 보였습니다. 마우스에 HD/Se/ICG gel을 주입 후 정해진 시간에 Visque InVivo Smart로 형광 촬영을 하여 생체 내에서의 생분해성을 확인하였습니다.
학술지: Journal of Controlled Release
2020 Apr 15;S0168-3659(20)30237-6.

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Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy 2020 인비보이미징시스템

제목: Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy
상세 내용: 본 논문에서는 gold nanoparticles (AuNPs)에 siRNA를 붙여 암 치료에 활용하는 방법을 소개하였습니다. AuNP로는 높은 수준의 세포 자살을 유도하고, siRNA로는 종양 유전자의 활성을 억제함을 in vitro상에서 확인하였습니다. 그리고 in vivo 상에서는 해당 물질을 처리한 마우스에서 암 조직의 크기가 4주동안 급격하게 줄어드는 것을 확인하였습니다. 그리고, 해당 물질의 생체 내 분포를 Visque InVivo Smart로 in vivo imaging을 하여 확인하였습니다.
학술지: Pharmaceutics
2020 Mar; 12(3): 261.

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Multifunctional Dendrimer-Entrapped Gold Nanoparticles for Labeling and Tracking T Cells Via Dual-Modal Computed Tomography and Fluorescence Imaging 2020 인비보이미징시스템

제목: Multifunctional Dendrimer-Entrapped Gold Nanoparticles for Labeling and Tracking T Cells Via Dual-Modal Computed Tomography and Fluorescence Imaging
상세 내용: 본 논문에서는 T cell 면역 치료법의 중요한 대상인 T cell을 효과적으로 관찰, 추적할 수 있는 nanoparticle을 새로이 개발하여 그 효능을 computed tomography (CT)와 형광 촬영으로 관찰, 증명하였습니다. 특히, in vivo 상태에서의 사용성을 확인하기 위하여, 실험 동물에 nanoparticle을 주입한 후, Visque InVivo Smart로 형광 in vivo imaging을 하여 활성화된 T cell을 형광으로 효과적으로 관찰할 수 있음을 확인하였습니다.
학술지: Biomacromolecules
2020 Apr 13;21(4):1587-1595.

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Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design 2019 인비보이미징시스템

제목: Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design
상세 내용: Aminoacyl-tRNA synthetase complex-interacting multifunctional protein 1 (AIMP1)-derived peptide (AdP)는 fibroblast를 활성화하고(FA), melanocyte를 억제하여(MI), 피부의 항노화용 화장품 성분으로 개발되었습니다. 하지만, 낮은 투과율로 AdP의 국소 전달을 위한 알맞은 방법이 필요했습니다. 이 연구에서는 fibroblast와 melanocyte에 대한 AdP의 몇몇 fragment의 효과를 시험하고, hydrosol을 기반으로 국소 전달을 위한 첨가제를 개발하고 최적화하였습니다. 이렇게 개발된 FA-AdP-loaded hydrosol은 Strat-M membrane (STM)에서 기존 FA-AdP보다 127배 높은 침착을 보였습니다. FA-AdP-loaded hydrosol을 형광으로 표지하고 인조 피부에 적용한 후, Visque InVivo Smart로 근적외선 형광 촬영하여 피부에 침작된 FA-AdP-loaded hydrosol의 양을 정량하였습니다.
학술지: Molecules 2019 May 22;24(10):1967

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Smart Microcapsules with Molecular Polarity- and Temperature-Dependent Permeability 2019 인비보이미징시스템

제목: Smart Microcapsules with Molecular Polarity- and Temperature-Dependent Permeability
상세 내용: 분자 선택적인 투과능을 가지는 microcapsule은 microreactor, 캡슐형 센서, 약물 및 세포 운반체, 인조 세포 등 다양한 형태로 활용할 수 있습니다. 본 논문에서는 두 종류의 monomers와 dodecanol을 이용하여, water-in-oil-in-water (W/O/W) double-emulsion 형태의 oil shell을 만들었습니다. 이렇게 만들어진 oil shell은 특정 온도 이상에서 선택적인 투과능을 보였습니다. oil shell 안에 ICG를 넣은 후, 마우스의 뒷다리 허벅지에 피하 주사한 후, 정해진 시간에 Visque InVivo Smart로 in vivo 형광 촬영하여 체내에서의 분비 효과를 확인하였습니다.
학술지: Small (Nano Micro Small) 2019 May;15(21):e1900434

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Oral Delivery of Honokiol Microparticles for Nonrapid Eye Movement Sleep 2019 인비보이미징시스템

제목: Oral Delivery of Honokiol Microparticles for Nonrapid Eye Movement Sleep
상세 내용: Honokiol (HNK)은 Magnolia Officinalis에서 추출한 물질로, nonrapid eye movement (NREM)을 촉진하는 것으로 알려져 있지만, 경구 투여 시 매우 낮은 생물학적 이용도로 불면증의 치료에는 사용되지 않고 있습니다. 본 논문에서는 HNK를 poly lactide-glycolide acid microparticles과 합성(HNK-MP)하여 생물학적 이용도를 매우 향상시켰습니다. 경구 투여 후 HNK-MP는 HNK에 비해 AUC0-12 h에서 15배 향상되었으며, maximum blood concentration (Cmax) 역시 6.5배 상승하였습니다. HNK-MP의 경구 투여는 NRME 수면을 60% 증가시켰습니다. 생물학적 안정성은 in vivo 실험을 통해 확인되었으며, 위장관의 손상도 발생하지 않았습니다. HNK-MP를 경구 투여한 후, 정해진 시간에 위장관을 적출, Visque InVivo Elite로 ex vivo 촬영하여 위장관 내에서의 정체를 확인하였습니다.
학술지: Molecular Pharmaceutics 2019 Feb 4;16(2):737-743

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Biodegradable Mesoporous Silica Achieved via Carbon Nanodots-Incorporated Framework Swelling for Debris-Mediated Photothermal Synergistic Immunotherapy 2019 인비보이미징시스템

제목: Biodegradable Mesoporous Silica Achieved via Carbon Nanodots-Incorporated Framework Swelling for Debris-Mediated Photothermal Synergistic Immunotherapy
상세 내용: 본 논문에서는 암의 photothermal therapy (PTT) 및 immunotherapy를 위하여 carbon nanodot (CD)를 mesoporous silica nanoparticles (MSNs)에 결합한 CD@MSNs를 개발하여, 실험 동물에서 그 효과를 확인하였습니다. 개발한 CD@MSNs를 실험 동물에 적용하여 PTT의 효과 및 전이 암에 대한 면역 치료 효과를 확인하였습니다. 특히, nanoparticle을 체내 주입한 후, 정해신 시간에 적출한 각 장기를 Visque InVivo Smart로 형광 촬영, 정량하여 시간에 따른 분포를 추적하였습니다.
학술지: Nano Letters
2019 Dec 11;19(12):8409-8417.

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Osteogenesis and angiogenesis are simultaneously enhanced in BMP2-/VEGF-transfected adipose stem cells through activation of the YAP/TAZ signaling pathway. 2019 인비보이미징시스템

제목: Osteogenesis and angiogenesis are simultaneously enhanced in BMP2-/VEGF-transfected adipose stem cells through activation of the YAP/TAZ signaling pathway.
상세 내용: 본 논문에서는 실험동물에 각각 BMP2와 VEGF를 가진 줄기 세포를 혼합, 주입하여 뼈 재생 및 혈관 재생 효과를 보이는지 확인하고, 최적의 혼합 비율을 찾았습니다. 특히, 이식한 줄기세포가 이식 장소에서 잘 유지되는지 Visque InVivo Elite로 in vivo imaging을 하여 추적, 확인하였습니다.
학술지: Biomaterials Science
2019 Nov 1;7(11):4588-4602.

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Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance 2019 인비보이미징시스템

제목: Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance
상세 내용: 본 논문에서는 비만 모델 실험동물에 백색 지방세포의 Fabp4를 목표로 한 CRISPR interference system을 주입하여 몸무게 및 지방량 등의 변화를 추적, 효과를 확인하였습니다. 특히, CRISPRi system이 목표로 한 지방세포 조직에 효과적으로 전달되었는지 Visque InVivo Smart로 ex vivo imaging을 하여 확인하였습니다.
학술지: Genome Research
2019 Sep;29(9):1442-1452.

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Conjugation of prostate cancer-specific aptamers to polyethylene glycol-grafted polyethylenimine for enhanced gene delivery to prostate cancer cells 2019 인비보이미징시스템

제목: Conjugation of prostate cancer-specific aptamers to polyethylene glycol-grafted polyethylenimine for enhanced gene delivery to prostate cancer cells
상세 내용: 본 논문에서는 전립선암에 specific한 aptamer (Wy5a)를 polyethylene glycol (PEG) linker를 활용 polyethylenimine (PEI)에 conjugate하여 전립선암을 xenograft한 마우스에 꼬리정맥 주사하여 암조직에 효과적으로 유전자를 전달할 수 있는지 Visque InVivo Smart로 ex vivo imaging을 하여 확인하였습니다.
학술지: Journal of Industrial and Engineering Chemistry
2019 May 25;73:182-191

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A nanoscale photothermal agent based on a metal-organic coordination polymer as a drug-loading framework for effective combination therapy 2019 인비보이미징시스템

제목: A nanoscale photothermal agent based on a metal-organic coordination polymer as a drug-loading framework for effective combination therapy
상세 내용: 본 논문에서는 photothermal agent의 체내 분포를 Visque InVivo Elite를 사용하여 in vivo 및 ex vivo imaging 후 정량하여 시간에 따른 체내 분포를 확인, photothermal agent가 암 조직에 축적됨을 확인하였습니다.
학술지: Acta Biomaterialia
2019 Aug;94:435-446.

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pH-Responsible Fluorescent Carbon Nanoparticles for Tumor Selective Theranostics via pH-turn on/off Fluorescence and Photothermal Effect In Vivo and In Vitro 2018 인비보이미징시스템

제목: pH-Responsible Fluorescent Carbon Nanoparticles for Tumor Selective Theranostics via pH-turn on/off Fluorescence and Photothermal Effect In Vivo and In Vitro
상세 내용: 본 논문에서는 암세포를 감지하는 동시에 near infrared (NIR) 파장의 photothermal therapy (PTT)를 위한 probe로, photothermal dye (IR825)와 carbonized zwitterionic polymer [FNP-I]로 만들어진 nanoparticle을 개발하였다. 해당 nanoparticle은 pH에 반응하는 형광 probe로 암세포를 감지하고, near infrared (NIR) 파장의 photothermal therapy (PTT)가 가능하다. 암세포의 주위에서 FNP-1가 활성화되어 형광이 나오고, IR825를 방출하여 PTT를 촉진한다. 암조직을 가진 마우스를 활용한 in vivo 실험에서 제작한 nanoparticle을 혈관 주사하여 확인한 결과, 형광 신호와 pH에 반응하는 PTT 효과를 확인하였습니다. Visque InVivo Elite로 실험용 마우스에 nanoparticle을 혈관 주사한 후, in vivo 촬영하여 주입 후 시간에 따른 체내 분포와, 장기를 적출한 후 ex vivo 촬영하여 암 조직에 축적되는 것을 확인하였습니다.
학술지: Nanoscale 2018 Feb 1;10(5):2512-2523

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Age-related changes in pial arterial structure and blood flow in mice 2016 인비보이미징시스템

제목: Age-related changes in pial arterial structure and blood flow in mice
상세 내용: 본 논문에서는 실험 동물의 뇌 혈관 구조 및 혈류를 Clevue의 실시간 형광 영상 쵤영 기능과 feature map 기능을 사용하여 분석하여 나이에 따른 변화를 확인하였습니다.
학술지: Neurobiology of Aging.
2016 Jan;37:161-170

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Exendin-4 protects hindlimb ischemic injury by inducing angiogenesis 2015 인비보이미징시스템

제목: Exendin-4 protects hindlimb ischemic injury by inducing angiogenesis
상세 내용: 본 논문에서는 허혈 조직이 있는 실험동물에 exendin-4를 주입한 후, 허혈조직에서의 혈류 흐름을 CleVue의 실시간 형광 영상 촬영 기능과 feature map 기능 등을 사용하여 분석하여 extendin-4의 angiogenesis 효과를 확인하였습니다.
학술지: Biochemical and Biophysical Research Communications.
2015 Oct 2;465(4):758-63.

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Use of indocyanine green for optical analysis of cortical infarcts in photothrombotic ischemic brains 2015 인비보이미징시스템

제목: Use of indocyanine green for optical analysis of cortical infarcts in photothrombotic ischemic brains
상세 내용: 본 논문에서는 빛을 이용하여 유도한 뇌경색에서 뇌경색 후 시간에 따른 회복 정도 및 혈류의 흐름을 실시간 형광 영상, FluAngio Map 및 DyAngio Map 등을 이용, 분석하여 혈류의 흐름과 회복 정도의 관계성을 확인하였습니다. 해당 분석 방법은 뷰웍스와 경희 의대에서 공동으로 연구에 참여하였고, CleVue를 사용하여 이와 동일하게 분석할 수 있습니다.
학술지: Journal of Neuroscience Methods.
2015 Jun 15;248:46-50

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Optical measurement of mouse strain differences in cerebral blood flow using indocyanine green 2015 인비보이미징시스템

제목: Optical measurement of mouse strain differences in cerebral blood flow using indocyanine green
상세 내용: 본 논문에서는 마우스의 대뇌 혈류를 실시간 형광 영상 및 FluAngio Map(+Trising 또는 +Imax)으로 분석, 비교하여 품종에 따른 차이를 확인하였습니다. 분석 방법은 뷰웍스와 경희 의대에서 공동으로 연구에 참여하였고, CleVue를 사용하여 이와 동일하게 분석할 수 있습니다.
학술지: Journal of Cerebral Blood Flow & Metabolism.
2015 Jun;35(6):912-6

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Noninvasive Optical Measurement of Cerebral Blood Flow in Mice Using Molecular Dynamics Analysis of Indocyanine Green 2012 인비보이미징시스템

제목: Noninvasive Optical Measurement of Cerebral Blood Flow in Mice Using Molecular Dynamics Analysis of Indocyanine Green
상세 내용: 본 논문에서는 마우스의 대뇌 혈류를 ICG와 실시간 형광 영상을 이용하여 촬영 후 dynamics 등을 분석하여 여러가지 혈류 특성을 측정하였습니다. 해당 분석 방법은 CleVue를 사용하여 동일하게 분석할 수 있습니다.
학술지: PLOS ONE.
2012;7(10):e48383.

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Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature 2011 인비보이미징시스템

제목: Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature
상세 내용: 본 논문에서는 ICG와 실시간 형광 영상을 이용하여 암 조직의 혈류를 촬영 후 dynamics 등을 분석하여 암 조직과 정상 조직간의 혈류 차이를 연구하였습니다. 해당 분석 방법은 CleVue를 사용하여 동일하게 분석할 수 있습니다.
학술지: Journal of Biomedical Optics
2011 Apr;16(4):046008.

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Efficient differentiation of human pluripotent stem cells into functional CD34 progenitor cells by combined modulation of the MEK/ERK and BMP4 signaling pathways 2010 인비보이미징시스템

제목: Efficient differentiation of human pluripotent stem cells into functional CD34 progenitor cells by combined modulation of the MEK/ERK and BMP4 signaling pathways
상세 내용: 본 논문에서는 실시간 형광 영상 촬영 등을 활용하여 허혈 조직에서의 혈류의 흐름을 이미징 후, 해당 조직에서의 dynamics 등을 분석하여 줄기 세포 치료법을 이용한 혈관 생성 효과를 확인하였습니다.해당 분석 방법은 CleVue를 활용하여 동일하게 분석할 수 있습니다.
학술지: BLOOD.
2010 Dec16;116(25):5762-72

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Unsorted human adipose tissue-derived stem cells promote angiogenesis and myogenesis in murine ischemic hindlimb model 2010 인비보이미징시스템

제목: Unsorted human adipose tissue-derived stem cells promote angiogenesis and myogenesis in murine ischemic hindlimb model
상세 내용: 본 논문에서는 허혈 조직이 있는 실험 동물을 줄기세포로 처리한 후, 허혈 조직에서의 혈류 흐름 등을 dynamics 등으로 분석하여 혈관 생성 및 근육 생성이 촉진되는 걸 확인하였습니다. 해당 분석 방법은 CleVue를 활용하여 동일하게 분석할 수 있습니다.
학술지: Microvascular Research.
2010 Dec;80(3):310-6.

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Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics 2009 인비보이미징시스템

제목: Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics
상세 내용: 본 논문에서는 실시간 형광 영상 촬영과 분석 기능을 활용하여 허혈 조직에서의 혈류의 흐름을 분석, 혈류의 흐름과 괴사 정도와의 관계를 조사하였습니다. 해당 분석 방법은 CleVue를 활용하여 동일하게 분석할 수 있습니다.
학술지: PLOS One. 2009;4(1):e4275.

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