纸类 | 生物成像

Regulation of cerebral blood flow boosts precise brain targeting of vinpocetine-derived ionizable-lipidoid nanoparticles 2024

标题: Regulation of cerebral blood flow boosts precise brain targeting of vinpocetine-derived ionizable-lipidoid nanoparticles
说明: In this study, researchers demonstrated that vinpocetine-derived ionizable-lipidoid nanoparticles (VIP) effectively enhance brain targeting. In the brain-targeting experiment, LNP@DiD was administered to healthy mice via the tail vein. Fluorescence images were captured at specified time intervals (0.5 h, 1 h, 2 h, 4 h, and 8 h) using the VISQUE in vivo Smart-LF System. The nanoparticles containing VIP exhibited significantly higher fluorescence intensity in the brain compared to those without VIP, confirming their robust brain-targeting capability. Additionally, major organs were collected from the mice at each time point for ex vivo imaging. Mice treated with VIP showed stronger fluorescence signals in the brain compared to the positive control, GSH-LNP, which utilized glutathione (GSH) as the targeting agent.
期刊: Nat Commun. 2024; 15: 3987.

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BMP7-Loaded Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorate Liver Fibrosis by Targeting Activated Hepatic Stellate Cells 2024

标题: BMP7-Loaded Human Umbilical Cord Mesenchymal Stem Cell-Derived Small Extracellular Vesicles Ameliorate Liver Fibrosis by Targeting Activated Hepatic Stellate Cells
说明: "The study focuses on enhancing the healing properties of small extracellular vesicles derived from human umbilical cord mesenchymal stem cells (hucMSC-sEVs). Due to their tendency to non-specific distribution in vivo and be partially removed by monocular macrophages, natural hucMSC-sEVs are not fully effective when administered systemically. To address this, researchers have modified these vesicles at the genetic level to overexpress the anti-fibrotic gene bone morphogenic protein7(BMP7) in partner cell.
In study, two mice from each group received injections of different types of small extracellular vesicles (sEVs) into their tail veins. The researchers tracked the sEVs’ movement through the body using the VISQUE in vivo system at various intervals up to 72 hours after injection. The imaging revealed that the BMP7-loaded sEVs were effectively directed to the liver of the mice. These results demonstrated hucMSC-sEVs primarily accumulated in the liver and had a moderate ability to target activated hepatic stellate cells (aHSCs), which was further improved by incorporating BMP7 into the sEVs."
期刊: Int J Nanomedicine. 2024; 19: 3475–3495.

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Role of gut-derived bacterial lipopolysaccharide and peripheral TLR4 in immobilization stress-induced itch aggravation in a mouse model of atopic dermatitis 2024

标题: Role of gut-derived bacterial lipopolysaccharide and peripheral TLR4 in immobilization stress-induced itch aggravation in a mouse model of atopic dermatitis
说明: This study elucidates the mechanisms by which emotional stress exacerbates itch sensation and inflammation in the skin of patients with atopic dermatitis (AD). The researchers investigate the role of gut-derived bacterial components, specifically lipopolysaccharides (LPS), in this process, focusing on how chronic stress impacts intestinal barrier function and its subsequent effects on skin inflammation and itch in a mouse model of AD. VISQUE InVivo Smart LF was employed to visualize the whole-body distribution of Cy5.5-labeled bacterial LPS. On the final day of the daily immobilization stress (IMO) period, nude mice were anesthetized, and their rectums were isolated. Cy5.5-labeled LPS was injected into the proximal colon, and the abdominal wall was sutured. The imaging showed how bacterial LPS permeates and distributes in the body under chronic stress conditions, providing insights into the role of gut-derived LPS in exacerbating skin inflammation and itch in the context of chronic stress and AD.
期刊: Sci Rep. 2024; 14: 6263.

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Modulation of chronic obstructive pulmonary disease progression by antioxidant metabolites from Pediococcus pentosaceus: enhancing gut probiotics abundance and the tryptophan-melatonin pathway 2024

标题: Modulation of chronic obstructive pulmonary disease progression by antioxidant metabolites from Pediococcus pentosaceus: enhancing gut probiotics abundance and the tryptophan-melatonin pathway
说明: "This study focused on evaluating the therapeutic potential of probiotics for Chronic obstructive pulmonary disease (COPD), a condition closely associated with oxidative stress. They tested the probiotic Pediococcus pentosaceus SMM914 could alleviate COPD symptoms and slow disease progression.
The study used VISQUE InVivo Smart and CleVue software for NIR fluorescence imaging to track the colonization of the probiotic SMM914 in the body. Using Cy5.5-d-lys labeled SMM914 mouse measured at 15 min, 45 min, and 4 hours after oral administration. The results showed that 45 minutes post-administration, most of the bacteria were located in the jejunum. After 2.5 hours, the bacteria concentrated in the ileum and colon, and by 4 hours, the fluorescence was primarily in the ileum, suggesting that the bacteria were nearing excretion. Additionally, the supplementation with SMM914 not only increased serotonin production but also improved the activity of the DDC enzyme in an inflammatory setting, supporting the probiotic's potential therapeutic effects in managing COPD."
期刊: Gut Microbes. 2024; 16(1): 2320283.

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Promoting collateral formation in type 2 diabetes mellitus using ultra-small nanodots with autophagy activation and ROS scavenging 2024

标题: Promoting collateral formation in type 2 diabetes mellitus using ultra-small nanodots with autophagy activation and ROS scavenging
说明: The study investigates the potential of molybdenum disulfide nanodots (MoS2 NDs) in promoting collateral formation and improving prognosis in type 2 diabetes mellitus (T2DM) by enhancing endothelial autophagy and scavenging reactive oxygen species (ROS). The Vieworks in vivo flourescence imaging system was used to assess the biodistribution of Cy5.5-labeled MoS2 NDs in ischemic muscles of a hindlimb ischemia mouse model to observe how these nanodots localize in the body post-injection. The imaging showed successful biodistribution of MoS2 NDs to the ischemic sites, indicating their effective delivery to targeted areas in the body
期刊: J Nanobiotechnology. 2024; 22: 85.

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Pharmaceutical Manipulation of Mitochondrial F0F1‐ATP Synthase Enables Imaging and Protection of Myocardial Ischemia/Reperfusion Injury Through Stress‐induced Selective Enrichment 2023

标题: Pharmaceutical Manipulation of Mitochondrial F0F1‐ATP Synthase Enables Imaging and Protection of Myocardial Ischemia/Reperfusion Injury Through Stress‐induced Selective Enrichment
说明: This study aims to assess the ability of IR-780, a near-infrared dye, to image and protect myocardial tissue from ischemia/reperfusion (I/R) injury by selectively targeting the injured cardiomyocytes. VISQUE In Vivo Smart-LF was employed to visualize IR-780’s accumulation in myocardial tissue in a pig I/R model, aiding in the real-time imaging of the injury site. The imaging demonstrated selective retention of IR-780 in ischemic areas, allowing for effective visualization of at-risk tissue and showing a reduction in I/R-induced cell death, which suggests potential cardioprotective effects.
期刊: Adv Sci (Weinh). 2023 Dec 14;11(9):2307880.

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Imaging assessment of photosensitizer emission induced by radionuclide-derived Cherenkov radiation using charge-coupled device optical imaging and long-pass filters 2023

标题: Imaging assessment of photosensitizer emission induced by radionuclide-derived Cherenkov radiation using charge-coupled device optical imaging and long-pass filters
说明: This study seeks to find a cost-effective method to confirm the excitation of photosensitizers (PS) by radionuclide-derived Cherenkov radiation (CR) and distinguish the resulting fluorescence emission. Tetrakis (4-carboxyphenyl) porphyrin (TCPP) was selected as a model PS, and the beta-emitting isotope Copper-64 was used as a CR-producing radionuclide. VISQUE InVivo Smart was used to confirm the inherent fluorescence characteristics of TCPP emission with filters for blue light (390-490 nm) excitation and red light (690-740 nm) emission. Combining other experiments, the researchers conclude that a simple method utilizing a CCD optical imaging system with different high-transmission long-pass filters and subtraction image processing can effectively assess if photosensitizer candidates are excited by radionuclide-derived Cherenkov radiation to generate fluorescence emission.
期刊: World J Radiol. 2023 Nov 28; 15(11): 315–323.

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Development of a fibroblast activation protein-targeted PET/NIR dual-modality probe and its application in head and neck cancer 2023

标题: Development of a fibroblast activation protein-targeted PET/NIR dual-modality probe and its application in head and neck cancer
说明: This study developed the PET/NIR probe [68Ga]Ga-FAP-2286-ICG, targeting fibroblast activation protein (FAP) found in cancer-associated fibroblasts in solid tumors like head and neck squamous cell carcinoma (HNSCC). Fluorescence imaging using the VISQUE InVivo Smart-LF confirmed the probe's effectiveness in guiding surgical resections for precise tumor removal. U87MG tumor-bearing mice were imaged post-injection to identify the optimal timing for fluorescence imaging (FI), showing significant contrast as early as 0.5 hours, peaking at 24 hours and remaining elevated until 72 hours, indicating the ideal window for surgery. Unlike free ICG, which showed no tumor fluorescence, FAP-2286-ICG demonstrated enhanced targeting and retention. The imaging also revealed significant fluorescence in the liver and kidneys, indicating metabolic processing, and residual fluorescence post-surgery highlighted its potential for detecting remaining cancerous tissues.
期刊: Front Bioeng Biotechnol. 2023 Nov 3;11:1291824.

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Rapid and Widespread Distribution of Intranasal Small Extracellular Vesicles Derived from Mesenchymal Stem Cells throughout the Brain Potentially via the Perivascular Pathway 2023

标题: Rapid and Widespread Distribution of Intranasal Small Extracellular Vesicles Derived from Mesenchymal Stem Cells throughout the Brain Potentially via the Perivascular Pathway
说明: The study aims to explore the distribution and potential therapeutic benefits of small extracellular vesicles (sEVs) derived from mesenchymal stem cells (MSCs) when administered intranasally, emphasizing their rapid and extensive delivery through the perivascular pathway. A VISQUE In Vivo Smart-LF system was utilized to image the distribution of Cy7-labeled MSC-sEVs in mice at 0.5, 2, 6, and 24 hours post-intranasal administration. Additionally, ex vivo imaging was performed to compare delivery to different organs. This data confirmed the fast and centralized distribution of intranasal MSC-sEVs.
期刊: Pharmaceutics. 2023 Nov; 15(11): 2578.

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Exosomal mRNAs for Angiogenic–Osteogenic Coupled Bone Repair 2023

标题: Exosomal mRNAs for Angiogenic–Osteogenic Coupled Bone Repair
说明: The main goal of the study is to develop a scalable and efficient method for producing therapeutic small extracellular vesicles (sEVs) loaded with mRNAs for bone tissue regeneration. The Vieworks VISQUE in vivo Smarr-LF was employed to perform fluorescence imaging of PKH26-labeled sEVs within the PEGS-A hydrogel and standard scaffolds in a rat model. The imaging showed strong PKH26 signals, indicating successful delivery and distribution of the sEVs. Notably, the PEGS-A hydrogel demonstrated significantly higher fluorescence intensity compared to the standard scaffold after three days. This imaging confirmed that the sEVs were retained and released more effectively within the hydrogel, leading to sustained therapeutic presence at the defect site over a two-week period.
期刊: Adv Sci (Weinh). 2023 Oct 17;10(33):2302622.

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Sustained delivery of triamcinolone acetonide from a thermosensitive microemulsion gel system for the treatment of sensorineural hearing loss 2023

标题: Sustained delivery of triamcinolone acetonide from a thermosensitive microemulsion gel system for the treatment of sensorineural hearing loss
说明: The study aims to develop and evaluate a thermosensitive microemulsion gel (MEG) system for the sustained delivery of triamcinolone acetonide (TA) to treat sensorineural hearing loss (SNHL) by improving drug retention and therapeutic efficacy in the inner ear. The VISQUE InVivo Smart-LF system was used to monitor the in vivo distribution and retention of Cy7-labeled TA-MEG in the inner ear of mice after intratympanic administration. This imaging system helped track the gel's localization and persistence over time. Imaging results demonstrated that the TA-MEG formulation exhibited sustained retention in the cochlea, with significant localization observed up to 6 days post-administration. This prolonged presence indicated effective gelation and slow degradation, validating the MEG system's potential for sustained drug delivery in SNHL treatment.
期刊: Drug Deliv. 2023; 30(1): 2242003.

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Site-selective superassembly of biomimetic nanorobots enabling deep penetration into tumor with stiff stroma 2023

标题: Site-selective superassembly of biomimetic nanorobots enabling deep penetration into tumor with stiff stroma
说明: The primary objective of the study is to develop and evaluate a new class of nanorobots designed to improve tumor penetration and drug delivery for treating triple-negative breast cancer (TNBC). The VISQUE InVivo Elite was used to monitor tumor growth and evaluate the distribution of UHHTN(Urchin Head/Hollow Tail Nanostructures)/DOX(Doxorubicin) nanorobots in mouse models. Researchers conducted bioluminescence imaging to monitor tumor progression and performed ex vivo imaging of various organs at different time intervals after the nanorobot injection. This imaging approach provided detailed insights into the accumulation of the nanorobots in the tumor compared to other tissues, confirming their targeted delivery and enhancing understanding of their therapeutic efficacy. The results showed that the UHHTN/DOX nanorobots, combined with photothermal therapy, significantly reduced tumor growth and improved survival rates in treated mice, demonstrating their potential for effective cancer treatment.
期刊: Nat Commun. 2023 Aug 2;14:4628.

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Activation of autophagy by in situ Zn2+ chelation reaction for enhanced tumor chemoimmunotherapy 2023

标题: Activation of autophagy by in situ Zn2+ chelation reaction for enhanced tumor chemoimmunotherapy
说明: The main goal of this study is to develop a nanoplatform that activates autophagy through in situ zinc ion (Zn²⁺) chelation, aiming to enhance the efficacy of tumor chemoimmunotherapy. VISQUE InVivo Elite was used to monitor the biodistribution and metabolism of Cy5.5-labeled DSF@Zn-DMSNs in tumor-bearing mice at various time points post-administration. Imaging results showed that the DSF@Zn-DMSNs had widespread and prolonged retention in tumor tissues. This retention confirmed the effective targeting and accumulation of the nanoparticles within the tumor, which correlated with the enhanced therapeutic outcomes observed in the study, including significant tumor growth inhibition and improved immune responses.
期刊: Bioact Mater. 2023 Nov; 29: 116–131.

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Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics 2023

标题: Discovery of Intratumoral Oncolytic Bacteria Toward Targeted Anticancer Theranostics
说明: The study aimed to investigate the herapeutic potential of tumor-resident bacteria and their association with photosynthetic bacteria for targeted cancer immunotherapy. VISQUE InVivo Smart-LF was employed to monitor the near-infrared fluorescence (NIR FL) emitted by the bacteria in tumor-bearing mice, providing insights into their targeting efficacy and biodistribution. Mice received intravenous injections of bacterial suspensions, and imaging was performed five days post-injection to visualize the NIR FL intensity across the entire body, specifically focusing on tumor sites. The results indicated a strong tumor-targeting effect, with significant fluorescence observed in targeted tumors compared to other tissues, highlighting the effectiveness of the bacterial treatments in suppressing tumor growth and inducing durable antitumor immunity. Overall, the imaging demonstrated that these bacteria could serve as a promising therapeutic strategy for cancer treatment.
期刊: Adv Sci (Weinh). 2023 May 7;10(20):2301679.

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Synergistic Encapsulation of Paclitaxel and Sorafenib by Methoxy Poly(Ethylene Glycol)-b-Poly(Caprolactone) Polymeric Micelles for Ovarian Cancer Therapy 2023

标题: Synergistic Encapsulation of Paclitaxel and Sorafenib by Methoxy Poly(Ethylene Glycol)-b-Poly(Caprolactone) Polymeric Micelles for Ovarian Cancer Therapy
说明: The main goal of this study is to develop and evaluate methoxy poly(ethylene glycol)-b-poly(caprolactone) (mPEG-b-PCL) polymeric micelles for the co-delivery of paclitaxel (PTX) and sorafenib (SRF) to enhance the treatment efficacy against ovarian cancer (OC) by exploiting the synergistic effects of these two drugs. Smart-LF was used in this study to monitor the in vivo bioluminescent imaging (BLI) of tumors in xenograft nude mice models. This system helped evaluate the tumor growth inhibition (TGI) percentage before and after drug administration. The imaging results using Smart-LF showed that the PTX/SRF micelle formulation exhibited significant tumor growth inhibition compared to the control and other treatment groups. The bioluminescence signal intensity measurements indicated effective suppression of tumor growth in the experimental groups treated with the micellar formulation.
期刊: Pharmaceutics. 2023 Apr; 15(4): 1206.

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Efficacy of recombinant Bacillus Calmette-Guérin containing dltA in in vivo three-dimensional bio-printed bladder cancer-on-a-chip and ex vivo orthotopic mouse model 2023

标题: Efficacy of recombinant Bacillus Calmette-Guérin containing dltA in in vivo three-dimensional bio-printed bladder cancer-on-a-chip and ex vivo orthotopic mouse model
说明: The main goal of the paper is to evaluate the efficacy and optimal dosage of recombinant Bacillus Calmette-Guérin containing dltA (rBCG-dltA) in treating bladder cancer, using both a three-dimensional bio-printed bladder cancer-on-a-chip model and an orthotopic bladder cancer mouse model. The Vieworks VISQUE InVivio Smart-LF was used to monitor bioluminescence in the mouse bladder, enabling researchers to observe tumor progression and regression after treatment with rBCG-dltA. Smart-LF demonstrated that the rBCG-dltA treatment, particularly at higher dosages, significantly reduced tumor intensity compared to controls, indicating its potential antitumor efficacy(icu-64-296).
期刊: Investig Clin Urol. 2023 Mar 29;64(3):296–305

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Wavelength engineerable porous organic polymer photosensitizers with protonation triggered ROS generation 2023

标题: Wavelength engineerable porous organic polymer photosensitizers with protonation triggered ROS generation
说明: The primary objective of this research is to develop a wavelength-engineerable, protonation-triggered porous organic polymer (POP) that can efficiently generate reactive oxygen species (ROS) for applications in biomedicine and photocatalysis. The focus is on creating a novel imidazolium-based POP that facilitates ROS generation without relying on metal ions or heavy atoms, addressing the challenge of maintaining high ROS yield while allowing for tunable excitation wavelengths. VISQUE InVivo Elite was used for the fluorescence imaging of U87MG-xenograft mice. The mice were imaged 24 hours after they were administered PBS, KUP-1, and O2@KUP-1 to assess the biodistribution and effectiveness of the developed photosensitizer. The imaging data indicated that O2@KUP-1 showed enhanced fluorescence signals compared to KUP-1 and PBS, demonstrating the effectiveness of the POP in generating ROS in vivo. This result underscores the potential of the developed polymer for applications in targeted cancer therapies and other biomedical fields.
期刊: Nat Commun. 2023; 14: 1498.

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Novel NF-κB reporter mouse for the non-invasive monitoring of inflammatory diseases 2023

标题: Novel NF-κB reporter mouse for the non-invasive monitoring of inflammatory diseases
说明: "The main goal of the study was to create and evaluate a novel NF-kB luciferase reporter mouse model that enables non-invasive, real-time tracking of inflammatory responses in whole-body and cell-specific contexts.
The Vieworks VISQUE InVivo ART100 imaging system was used to capture bioluminescence signals in the mice, allowing researchers to visualize inflammation progression in different models, such as those induced by lipopolysaccharide (LPS) or diet. The result showed increased bioluminescence in targeted tissues correlating with inflammatory activity, validating the reporter mice’s capacity to reflect inflammation accurately and dynamically."
期刊: Sci Rep. 2023 Mar 2;13:3556.

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Bone tumor-homing nanotherapeutics for prolonged retention in tumor microenvironment and facilitated apoptotic process via mevalonate pathway inhibition 2023

标题: Bone tumor-homing nanotherapeutics for prolonged retention in tumor microenvironment and facilitated apoptotic process via mevalonate pathway inhibition
说明: This paper aims to create and assess bone tumor-targeting polymeric nanotherapeutics (NTs) designed to enhance drug retention within the tumor microenvironment (TME) and improve the effectiveness of bone cancer treatments. The research involves the use of alendronate-decorated chondroitin sulfate A-graft-poly(lactide-co-glycolide) (PLCSA-AD) combined with the chemotherapy drug doxorubicin (DOX) to inhibit the mevalonate pathway, a key metabolic process in tumor cells, thereby inducing apoptosis (cell death) and enhancing therapeutic outcomes. VISQUE InVivo Smart was employed to visualize and verify the accumulation of PLCSA-AD nanotherapeutics in bone tumor tissue in a mouse xenograft model. Cy5.5-labeled NTs were injected into the tail vein of tumor-bearing mice, with imaging performed at 0, 1, 3, 5, 9, and 24 hours post-injection. Subsequently, the tumors and major organs were dissected for ex vivo analysis. The imaging results revealed a significant increase in tumor accumulation of the PLCSA-AD nanotherapeutics, showing a 2.64-fold higher radiant efficiency at 24 hours and a 1.73-fold higher tumor disposition ex vivo compared to the non-alendronate-decorated variant (PLCSA).
期刊: Mater Today Bio. 2023 Apr; 19: 100591.

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Endocrine modulation of brain-skeleton axis driven by neural stem cell-derived perilipin 5 in the lipid metabolism homeostasis for bone regeneration 2023

标题: Endocrine modulation of brain-skeleton axis driven by neural stem cell-derived perilipin 5 in the lipid metabolism homeostasis for bone regeneration
说明: "The primary aim of the paper is to explore how neural stem cell-derived exosomes, particularly those containing perilipin 5 (PLIN5), contribute to lipid metabolism regulation to enhance bone regeneration via an endocrine axis linking the brain and skeleton.
The Vieworks VISQUE InVivo Elite imaging system was used to track fluorescently labeled neural stem cell-derived exosomes as they traveled from the brain to bone injury sites, allowing researchers to visualize their distribution and homing capabilities. Results confirmed that the exosomes reached the bone injury areas, highlighting their potential to aid bone regeneration by modulating lipid metabolism in metabolic disorder conditions."
期刊: Mol Ther. 2023 Feb 9;31(5):1293–1312.

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Overcoming the On‐Target Toxicity in Antibody‐Mediated Therapies via an Indirect Active Targeting Strategy 2023

标题: Overcoming the On‐Target Toxicity in Antibody‐Mediated Therapies via an Indirect Active Targeting Strategy
说明: The primary objective of this study is to address and mitigate the on-target toxicity that is often associated with antibody-mediated therapy, specifically targeting tumor-associated antigens. The researchers aim to develop a method to reduce the adverse effects of antibody treatment by using a strategy that involves masking the antibody's binding domain with a cleavable peptide, which is then selectively removed in the tumor microenvironment. This approach seeks to enhance the specificity and safety of antibody-mediated cancer therapies. VISQUE in vivo Smart-LF was used for the ex vivo images of the fluorescently labeled antibodies. This allowed for temporal observation of how the antibodies circulated and accumulated in the tumors versus normal tissues, providing critical data on the effectiveness of the masking strategy. The images demonstrated that the engineered antibodies, with the masking peptide, showed reduced accumulation in non-tumor tissues compared to the unmodified antibodies. This selective localization was crucial to confirm that the peptide masking approach effectively reduced on-target toxicity without compromising the antibody's ability to target tumors.
期刊: Adv Sci (Weinh). 2023 Mar; 10(9): 2206912.

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Repurpose dasatinib and quercetin: Targeting senescent cells ameliorates postmenopausal osteoporosis and rejuvenates bone regeneration 2023

标题: Repurpose dasatinib and quercetin: Targeting senescent cells ameliorates postmenopausal osteoporosis and rejuvenates bone regeneration
说明: "This study demonstrated that the combination of dadatinib and quercetin (DQ) can effectively improve postmenopausal osteoporosis (PMO) and enhance born health. Osteoporosis is one of the prevalnet chronic disease in the elderly, especially postmenopausal women. Current treatments for PMO often have shown adverse effects. DQ has shown potential in treating various age-related conditions. However, its effectiveness in PMO was not investigated until now.
VISQUE InVivo ART100 used to capture quantify fluorescence intensity of the released substance in vivo. The device was used to take images of the rat femoral defect area at different time points (1st, 3rd, and 5th day) to monitor the release of the substances. The fluorescence intensity of the released quercetin (using calcein as a model) and BMP2 (modified with sulfo-cyanine7) was measured. This study confirms that DQ can ameliorate PMO."
期刊: Bioact Mater. 2023 Jul; 25: 13–28.

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Nanoformulation of the K-Ras(G12D)-inhibitory peptide KS-58 suppresses colorectal and pancreatic cancer-derived tumors 2023

标题: Nanoformulation of the K-Ras(G12D)-inhibitory peptide KS-58 suppresses colorectal and pancreatic cancer-derived tumors
说明: The main goal of the paper is to develop and evaluate a nanoformulation of the K-Ras(G12D)-inhibitory peptide KS-58 for its effectiveness in suppressing colorectal and pancreatic cancer-derived tumors. The researchers aimed to improve the delivery and efficacy of the KS-58 peptide, which targets the K-Ras(G12D) mutation, a common and challenging mutation in these types of cancers. Vieworks VISQUE InVivo Smart-LF was used to monitor and evaluate the tumor growth and the therapeutic effect of the KS-58 nanoformulation in live animal models. The imaging with VISQUE InVivo Smart-LF provided clear bioluminescent images that allowed the researchers to assess the effectiveness of the KS-58 nanoformulation. The results showed significant suppression of tumor growth in the treated groups compared to the control groups. The data obtained from the imaging system supported the study's goal by demonstrating that the nanoformulated KS-58 peptide effectively inhibits tumor growth in colorectal and pancreatic cancer models, thus validating the therapeutic potential of this approach.
期刊: Sci Rep. 2023; 13: 518.

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Potential Alzheimer's disease therapeutic nano-platform: Discovery of amyloid-beta plaque disaggregating agent and brain-targeted delivery system using porous silicon nanoparticles 2023

标题: Potential Alzheimer's disease therapeutic nano-platform: Discovery of amyloid-beta plaque disaggregating agent and brain-targeted delivery system using porous silicon nanoparticles
说明: "This study introduces a new agent for breaking down amyloid-beta(Aβ) plaque and a targeted delivery system for Alzheimer's disease(AD) treatment usinf porous silicon nanoparticles(pSiNPs). The nano-formulation, named BiotiCaCl2-ANA-pSiNPs (BCAP), effectively delivered a significant amount of the therapeutic agent 6-amino-2-naphthalenesulfonic acid (ANA) to the AD brain. This led to the disaggregation of amyloid plaques and improved memory in an AD mouse model.
Fluorescence imaging with VISQUE InVivo Elit was used to monitor the distribution of BCAP in the body. The results indicated that BCAP accumulated significantly in the AD brain, demonstrating its superior brain-targeting capability. Imaging also confirmed that BCAP effectively disaggregated Aβ plaques in vivo, reducing their presence in the AD brain. The study demonstrated that BCAP disaggregated Aβ plaques with high efficacy in vitro and showed high biocompatibility in vivo, leading to improved memory and reduced inflammation."
期刊: Bioact Mater. 2023 Jun; 24: 497–506.

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DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma 2023

标题: DGKB mediates radioresistance by regulating DGAT1-dependent lipotoxicity in glioblastoma
说明: The study investigates the role of diacylglycerol kinase B (DGKB) in the radioresistance of glioblastoma (GBM) cells. The researchers seek to understand how the downregulation of DGKB, which regulates the intracellular concentration of diacylglycerol (DAG), contributes to the resistance of GBM cells to radiotherapy. By exploring the mechanisms of DGKB downregulation and its effects on lipid metabolism, the study aims to identify potential therapeutic targets to overcome GBM radioresistance. VISQUE in vivo SmartLF was employed for bioluminescent imaging to track the tumor size and progression after irradiation in mice implanted with luciferase-expressing GBM cells. This imaging visualized the effects of radiotherapy combined with DGKB overexpression on tumor growth. It was revealed that radiation alone had minimal effects on tumor growth. However, when combined with DGKB overexpression, there was a significant reduction in tumor growth by approximately 62.19%.
期刊: Cell Rep Med. 2023 Jan 17; 4(1): 100880.

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Development of an alginate-gelatin bioink enhancing osteogenic differentiation by gelatin release 2023

标题: Development of an alginate-gelatin bioink enhancing osteogenic differentiation by gelatin release
说明: This study aimed to enhance the osteogenic activity in a hydrogel by integrating gelatin, which supports both encapsulated and nearby cells. Methacrylate-modified alginate (MA-alginate) was selected for its low cell adhesion properties. VISQUE inVivo Smart system was used to monitor gelatin levels in hydrogel disks at several time points (0h, 1, 7, 14, and 21 days) using fluorescent imaging. By attaching fluorescein dye to gelatin, we assessed its release over time. The results showed that higher amounts of gelatin led to increased and gradual release of gelatin. This release improved the swelling properties, cell viability, and promote bone growth of the hydrogels compared to those made only of MA-alginate. Thus, the gelatin-containing alginate-based bioink developed could effectively support osteogenesis in bone tissue regeneration.
期刊: Int J Bioprint. 2023; 9(2): 660.

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Active Targeting of Versatile Nanocomplex Using the Novel Biomarker of Breast Cancer Stem Cells 2023

标题: Active Targeting of Versatile Nanocomplex Using the Novel Biomarker of Breast Cancer Stem Cells
说明: The paper investigates the activation of autophagy through in situ Zn²⁺ chelation reaction to enhance tumor chemoimmunotherapy. The researchers explore how Zn²⁺ chelation can induce autophagy and subsequently improve the efficacy of chemotherapeutic drugs and immune responses against tumors. VISQUE in vivo Smart-LF is used to monitor and analyze the biodistribution of a Zn²⁺ chelator (ZnDPA) in mice. Fluorescent imaging of CT-26 tumor bearing BALB/c mice after 0, 2, 4, 8, 12, 24, 48, and 72h after the intratumoral injection of Cy5.5-labeld DSF@Zn-DMSNs revealed that ZnDPA exhibited significant tumor targeting capability, as evidenced by the accumulation of strong fluorescent signals in the tumor regions. This successful targeting highlights the potential of Zn²⁺ chelation for enhancing the delivery and effectiveness of chemotherapy and immunotherapy in cancer treatment.
期刊: Int J Mol Sci. 2023 Jan; 24(1): 685.

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Enhancement of the therapeutic efficacy of the MAP regimen using thiamine pyrophosphate‐decorated albumin nanoclusters in osteosarcoma treatment 2023

标题: Enhancement of the therapeutic efficacy of the MAP regimen using thiamine pyrophosphate‐decorated albumin nanoclusters in osteosarcoma treatment
说明: "Current guidelines for Osteosarcoma recommend combination therapy rather than a single therapy. This study proposed that adding thiamine pyrophosphate (TPP) to nano-drug delivery systems could target bone tumors more effectively without increasing toxicity. The goal was to test whether replacing free methotrexate (MTX) and doxorubicin (DOX) with TPP-coated albumin nanoclusters could improve the effectiveness of the MAP regimen.
VISQUE InVivo Smart was used to monitor fluorescence signals from Cy5.5-labeled nanoclusters injected into mice. Whole-body body fluorescence was tracked using near-infrared fluorescence (NIRF) imaging at various time points: 0, 1, 2, 4, 6, 12, and 24 hours post-injection. After 24 hours, the system was also employed for ex vivo imaging of major organs and tumor tissues. The results showed that the albumin-based nanoclusters gradually accumulated in the osteosarcoma over time, with the TPP-decorated nanoclusters demonstrating improved tumor distributions. Thus, this study showed that the HSA(Human Serum Albumin)-TPP NCs-based MAP regimen may be a promising strategy for Osteosarcoma treatment."
期刊: Bioeng Transl Med. 2023 Nov; 8(6): e10472.

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Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy 2022

标题: Design of a self-driven probiotic-CRISPR/Cas9 nanosystem for sono-immunometabolic cancer therapy
说明: The study aims to develop and evaluate a novel nanosystem that combines probiotics (Lactobacillus rhamnosus GG) with CRISPR-Cas9 technology to target and treat cancer. The researchers harness the tumor-targeting properties of probiotics and the gene-editing capabilities of CRISPR-Cas9 to specifically attack cancer cells in hypoxic tumor environments, improving the efficacy of cancer therapy. VISQUE imaging system is employed to track and analyze the accumulation and distribution of the nanosystem labeled with cy5.5 within tumor-bearing mice. This fluorescence imaging allowed for real-time observation of how well the nanosystem targeted and accumulated in tumor tissues. The imaging results showed that the hybrid nanosystem (LGG-MHS-Cas9) effectively accumulated in tumor tissues, particularly in hypoxic regions, while showing minimal off-target accumulation in other organs. These findings confirm the nanosystem’s potential for improving the precision and effectiveness of cancer therapies by concentrating the therapeutic agents in the areas where they are most needed.
期刊: Nat Commun. 2022; 13: 7903.

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Nanorod/nanodisk‐integrated liquid crystalline systems for starvation, chemodynamic, and photothermal therapy of cancer 2022

标题: Nanorod/nanodisk‐integrated liquid crystalline systems for starvation, chemodynamic, and photothermal therapy of cancer
说明: "This study developed a hybrid gel indocyanine green (ICG), glucose oxidase (GOx), and copper(II) sulfate (Cu) with cellulose nanocrystal (CNC) and Laponite (LAP) was developed for targeted cancer therapy. The gel combines: Photothermal Therapy (PTT), Starvation Therapy (ST), Chemodynamic Therapy (CDT).
VISQUE InVivo Smart was used to evaluate ICG felease form the gel and its uptaken by CT-26 cancer cells, with fluorescence intensity measured at 0, 10, 30, 60, 120, 360, and 1440 min. The CNC/LAP gel with ICG effectively induces hyperthermia in tumors when exposed to near-infrared (NIR) laser, demonstrating improved retention and extended therapeutic effects. Overall, this gel shows promise for localized cancer treatment and maintains a high safety profile."
期刊: Bioeng Transl Med. 2023 Sep; 8(5): e10470.

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Modified exosomal SIRPα variants alleviate white matter injury after intracerebral hemorrhage via microglia/macrophages 2022

标题: Modified exosomal SIRPα variants alleviate white matter injury after intracerebral hemorrhage via microglia/macrophages
说明: The study aims to develop and evaluate modified exosomal SIRPα variants (SIRPα-v Exos) to alleviate white matter injury after intracerebral hemorrhage (ICH) by promoting the clearance of hematoma and regulating the immune response via microglia/macrophages. VISQUE™ Imaging System was used to monitor the biodistribution of DiR-labeled SIRPα-v Exosomes in mice after intravenous injection. This system tracked the exosomes’ accumulation in various organs over time. Imaging results showed that SIRPα-v Exosomes accumulated in the brain and other organs, with a particularly notable presence in the brain 8 hours post-injection. This confirmed the ability of the exosomes to cross the blood-brain barrier and contribute to hematoma clearance.
期刊: Biomater Res. 2022 Nov 26;26:67.

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Folate receptor-targeted near-infrared photodynamic therapy for folate receptor-overexpressing tumors 2022

标题: Folate receptor-targeted near-infrared photodynamic therapy for folate receptor-overexpressing tumors
说明: "Photodynamic therapy (PDT) is a promising minimally invasive cancer treatment that relies on photosensitizers (PS) and light exposure. Developing novel photosensitizers with optimal properties can significantly enhance PDT's effectiveness. Folate receptors (FR), which are overexpressed in about 40% of human cancers, are ideal targets for tumor-specific therapies. By conjugating photosensitizers with folate, specific targeting to FR can be achieved. The folate-linked near-infrared (NIR) probe, FolateSiR-1, has been developed to improve PDT by utilizing NIR light for targeted cancer treatment.
VISQUE InVivo Smart fluorescence imaging and analysis system was used to determine the fluorescence signal intensity (FI) of FilateSiR-1 in tumor-being mice, utilizing a HyperRed light filter set (Ex/Em = 630-680/690-740 nm). This system confirmed high fluorescence in KB tumors but not in OVCAR-3 or A4 tumors, indicating specific binding to folate receptors. Two hours post-injection, the fluorescence intensity peaked in KB tumors and diminished over time. In vivo and ex vivo imaging revealed significant tumor accumulation of FolateSiR-1 and reduced fluorescence in tumors subjected to photodynamic therapy (PDT), compared to untreated tumors. These results support the use of FolateSiR-1 as a promising photosensitizer for targeted PDT with minimal side effects."
期刊: World J Clin Oncol. 2022 Nov 24; 13(11): 880–895.

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ICAM1-Targeting Theranostic Nanoparticles for Magnetic Resonance Imaging and Therapy of Triple-Negative Breast Cancer 2022

标题: ICAM1-Targeting Theranostic Nanoparticles for Magnetic Resonance Imaging and Therapy of Triple-Negative Breast Cancer
说明: The study aims to design and evaluate ICAM1-targeting theranostic nanoparticles for enhanced magnetic resonance imaging (MRI) and treatment of triple-negative breast cancer (TNBC). The researchers focus on developing nanoparticles that can specifically target TNBC for both imaging and therapeutic purposes. VISQUE InVivo Smart-LF is used to monitor the biodistribution of Cy5.5-labeled nanoparticles in TNBC-bearing mice at various time points (1, 2, 4, and 24 hours post-injection). The imaging showed that the ICAM1-targeting nanoparticles exhibited efficient tumor accumulation compared to the control group. The fluorescence imaging demonstrated prolonged circulation time and enhanced targeting of the nanoparticles to TNBC tumors, confirming their potential for both diagnostic imaging and therapeutic efficacy.
期刊: Int J Nanomedicine. 2022 Nov 22;17:5605–5619.

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Multiplexed Imaging Reveals the Spatial Relationship of the Extracellular Acidity-Targeting pHLIP with Necrosis, Hypoxia, and the Integrin-Targeting cRGD Peptide 2022

标题: Multiplexed Imaging Reveals the Spatial Relationship of the Extracellular Acidity-Targeting pHLIP with Necrosis, Hypoxia, and the Integrin-Targeting cRGD Peptide
说明: "The study aimed to investigate the intratumoral distribution (ITD) of pH (low) insertion peptides (pHLIPs) and their spatial relationship with necrosis, hypoxia, and the integrin-targeting cRGD peptide in tumor models.
The VISQUE InVivo Smart-LF was used for in vivo and ex vivo near-infrared fluorescence (NIRF) imaging of tumor-bearing mice. The system helped visualize the distribution of IR800-pHLIP in the tumors at various time points post-injection. The imaging results showed that the ITD of pHLIPs was heterogeneous and had a high degree of colocalization with necrosis. The pHLIPs accumulated in necrotic regions and were spatially contiguous with hypoxia, providing insights into the potential theranostic applications of pHLIPs."
期刊: Cells. 2022 Nov 4;11(21):3499.

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NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma 2022

标题: NRBF2-mediated autophagy contributes to metabolite replenishment and radioresistance in glioblastoma
说明: The study aims to explore the role of NRBF2-mediated autophagy in glioblastoma (GBM) cells and how it contributes to metabolite replenishment and radioresistance. The researchers are trying to identify mechanisms through which autophagy can increase GBM cell survival during radiotherapy. With VISQUE Invivo Smart-LF, they perform bioluminescence imaging of tumor growth in GBM-bearing mice models. The researchers monitored tumor growth and assessed the effects of radiation on tumor development. The imaging showed that radiation treatment reduced tumor size, but tumors in the irradiated group exhibited higher expression of NRBF2 and proliferation markers, confirming its role in promoting radioresistance and tumor progression.
期刊: Exp Mol Med. 2022 Nov 4;54(11):1872–1885.

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Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment 2022

标题: Biomimetic nanoparticles drive the mechanism understanding of shear-wave elasticity stiffness in triple negative breast cancers to predict clinical treatment
说明: "The study aims to investigate the role of shear-wave elasticity (SWE) imaging stiffness in predicting treatment responses for triple-negative breast cancer (TNBC), focusing on how cancer-associated fibroblasts (CAFs) influence tumor elasticity and the effectiveness of therapy.
The Vieworks VISQUE Smart-LF in vivo imaging system was employed to capture SWE images of TNBC tumors, assessing their stiffness as part of the non-invasive imaging technique used to correlate tumor elasticity with clinical outcomes. Imaging with the Vieworks device showed that TNBC tumors had higher SWE stiffness, linked to increased CAF activity, fibrosis, and tumor hypoxia, factors that could potentially predict a tumor's response to neoadjuvant therapies."
期刊: Bioact Mater. 2022 Nov 3;22:567–587.

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HA-DOPE-Modified Honokiol-Loaded Liposomes Targeted Therapy for Osteosarcoma 2022

标题: HA-DOPE-Modified Honokiol-Loaded Liposomes Targeted Therapy for Osteosarcoma
说明: The paper aims to develop HA-DOPE-modified honokiol-loaded liposomes for targeted osteosarcoma therapy. The researchers seek to enhance the antitumor effects of honokiol (HNK) through improved delivery and targeting of osteosarcoma cells via interaction with the CD44 receptor. Using a VISQUE system, they observed the biodistribution of DiD-labeled liposomes in tumor-bearing mice. The fluorescence images showed that HA-DOPE-modified liposomes had significantly higher accumulation in the tumor compared to non-modified liposomes, demonstrating the effective targeting capability of the liposomal system to osteosarcoma cells via the CD44 receptor.
期刊: Int J Nanomedicine. 2022 Nov 1;17:5137–5151.

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Small molecule-assisted assembly of multifunctional ceria nanozymes for synergistic treatment of atherosclerosis 2022

标题: Small molecule-assisted assembly of multifunctional ceria nanozymes for synergistic treatment of atherosclerosis
说明: "The primary objective of the paper is to investigate the potential of multifunctional ceria nanozymes, combined with small molecules, to treat atherosclerosis by simultaneously targeting reactive oxygen species (ROS) and inflammation within atherosclerotic lesions.
The study utilized the Vieworks VISQUE Elit/Smart in vivo imaging system to perform fluorescence imaging, helping visualize the distribution of the nanozymes within atherosclerotic plaques in mice. The result confirmed the effective accumulation of the platelet membrane-coated ceria nanozyme complexes within inflamed atherosclerotic areas, highlighting their targeted delivery and therapeutic potential."
期刊: Nat Commun. 2022 Nov 1;13:6528.

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Lipid Profiles Obtained from MALDI Mass Spectrometric Imaging in Liver Cancer Metastasis Model 2022

标题: Lipid Profiles Obtained from MALDI Mass Spectrometric Imaging in Liver Cancer Metastasis Model
说明: The paper investigates lipid profiles in liver cancer metastasis using matrix-assisted laser desorption/ionization mass spectrometric imaging (MALDI-MSI) to identify potential biomarkers for metastasis, thereby improving diagnosis and treatment strategies. VISQUE in vivo Elite is used to monitor liver cancer metastasis progression in mice via tracking the localization of SK-Hep1_Luc cells in mouse liver tissues. The imaging results confirmed the accumulation of metastatic lesions in the liver. This allowed researchers to monitor tumor progression and validate the experimental metastasis model.
期刊: Int J Anal Chem. 2022 Oct 27;2022:6007158

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Exosomal Plasminogen Activator Inhibitor-1 Induces Ionizing Radiation-Adaptive Glioblastoma Cachexia 2022

标题: Exosomal Plasminogen Activator Inhibitor-1 Induces Ionizing Radiation-Adaptive Glioblastoma Cachexia
说明: "The study aims to elucidate the role of exosomal plasminogen activator inhibitor-1 (PAI-1) in inducing ionizing radiation-adaptive glioblastoma (GBM) cachexia and to explore the potential of targeting PAI-1 as a therapeutic strategy to manage GBM cachexia following radiotherapy.
VISQUE in vivo imaging system Smart-LF was used to monitor xenograft growth by bioluminescent imaging in the orthotopic xenograft mouse model. Results indicated that the total body weight and muscle fiber size of mice decreased after irradiation, indicating the development of cachexia in the irradiated GBM xenograft group."
期刊: Cells. 2022 Oct 1;11(19):3102.

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Huc-MSC-derived exosomes modified with the targeting peptide of aHSCs for liver fibrosis therapy 2022

标题: Huc-MSC-derived exosomes modified with the targeting peptide of aHSCs for liver fibrosis therapy
说明: The study aims to develop Huc-MSC-derived exosomes modified with a targeting peptide (HSTP1) to specifically target activated hepatic stellate cells (aHSCs) for the treatment of liver fibrosis. The goal is to enhance the delivery efficiency and therapeutic effects of exosomes on reversing aHSC activation and mitigating liver fibrosis. VISQUE in vivo Smart is used to track and analyze the biodistribution of DiR-labeled exosomes in rats. The fluorescence signals in the abdominal liver region are monitored at various time points post-injection. The imaging results showed that the HSTP1-modified exosomes (HSTP1-Exos) exhibited stronger fluorescence signals in the liver, indicating higher targeting efficiency to the aHSC region compared to unmodified exosomes.
期刊: J Nanobiotechnology. 2022 Oct 1;20:432.

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Effect of Pinoresinol and Vanillic Acid Isolated from Catalpa bignonioides on Mouse Myoblast Proliferation via the Akt/mTOR Signaling Pathway 2022

标题: Effect of Pinoresinol and Vanillic Acid Isolated from Catalpa bignonioides on Mouse Myoblast Proliferation via the Akt/mTOR Signaling Pathway
说明: This study investigates the effects of pinoresinol and vanillic acid, isolated from Catalpa bignonioides, on mouse myoblast proliferation, aiming to understand their potential in stimulating muscle growth via the Akt/mTOR signaling pathway. VISQUE InVivo Smart-LF was used to capture images of protein expression in Western blot analysis, allowing visualization of signaling pathway activation. Results showed that treatment with pinoresinol and vanillic acid notably increased protein phosphorylation within the Akt/mTOR pathway, supporting muscle cell proliferation and differentiation.
期刊: Molecules. 2022 Aug 24;27(17):5397

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Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma 2022

标题: Lenvatinib for effectively treating antiangiogenic drug-resistant nasopharyngeal carcinoma
说明: The study investigates lenvatinib, a multi-kinase inhibitor, as a treatment option for nasopharyngeal carcinoma (NPC) that has developed resistance to antiangiogenic drugs (AADs). It aims to understand the mechanisms of AAD resistance and evaluate lenvatinib’s effectiveness in overcoming this resistance by targeting both VEGFR2 and FGFR1 pathways. VISQUE Invivo Elite is used to detect GFP-positive metastatic nodules in the lungs of NPC-bearing mice, providing detailed insights into tumor growth and metastasis. The imaging showed significant suppression of metastasis in NPC-bearing mice treated with lenvatinib compared to anti-VEGF treatments, confirming lenvatinib's effectiveness in targeting the tumor vasculature and reducing metastasis
期刊: Cell Death Dis. 2022 Aug 19;13(8):724.

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Reactive oxygen species‐responsive dual‐targeted nanosystem promoted immunogenic cell death against breast cancer 2022

标题: Reactive oxygen species‐responsive dual‐targeted nanosystem promoted immunogenic cell death against breast cancer
说明: "The study aimed to develop a reactive oxygen species (ROS)-responsive dual-targeted nanosystem to promote immunogenic cell death (ICD) and enhance the therapeutic outcome against breast cancer.
The VISQUE InVivo imaging system was used to evaluate the tumor retention and targeting ability of the nanoparticles in a mouse xenograft model. The imaging results demonstrated that the nanoparticles displayed longer retention in the tumor zone and effectively accumulated in the tumor tissue, indicating their potential for targeted cancer therapy."
期刊: Bioeng Transl Med. 2022 Aug 3;8(5):e10379.

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Cardiomyocytes induced from hiPSCs by well-defined compounds have therapeutic potential in heart failure by secreting PDGF-BB 2022

标题: Cardiomyocytes induced from hiPSCs by well-defined compounds have therapeutic potential in heart failure by secreting PDGF-BB
说明: The study explores the therapeutic potential of cardiomyocytes induced from human-induced pluripotent stem cells (hiPSCs) using well-defined compounds, focusing on their ability to secrete PDGF-BB and promote myocardial repair in heart failure. VISQUE InVivo Smart-LF was used to perform fluorescence imaging in mice to monitor the biodistribution and homing of transplanted hiPS-CMs over 20 days. The imaging demonstrated that the hiPS-CMs accumulated at the site of injury in the heart, with a longer duration of fluorescent signal observed for the compound-induced hiPS-CMs compared to the standard group, supporting their enhanced therapeutic efficacy.
期刊: Signal Transduct Target Ther. 2022 Jul 29;7:253.

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Exosomal miR-17-5p from adipose-derived mesenchymal stem cells inhibits abdominal aortic aneurysm by suppressing TXNIP-NLRP3 inflammasome 2022

标题: Exosomal miR-17-5p from adipose-derived mesenchymal stem cells inhibits abdominal aortic aneurysm by suppressing TXNIP-NLRP3 inflammasome
说明: "The study aimed to investigate the role of exosomal miR-17-5p from adipose-derived mesenchymal stem cells (ADSCs) in inhibiting abdominal aortic aneurysm (AAA) progression by suppressing the TXNIP-NLRP3 inflammasome.
The VISQUE™ InVivo Smart-LF imaging system from Vieworks was used for in vivo fluorescence imaging to track the distribution of DR-labeled ADSC-derived exosomes in mice. Imaging results indicated that the exosomes were primarily localized in the liver, with notable accumulation also seen in the kidneys, lungs, and spleen."
期刊: Stem Cell Res Ther. 2022 Jul 26;13:349.

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Molecular imaging on ACE2‐dependent transocular infection of coronavirus 2022

标题: Molecular imaging on ACE2‐dependent transocular infection of coronavirus
说明: The study investigates the ACE2-dependent transocular infection pathway of SARS-CoV-2, utilizing molecular imaging to provide dynamic, quantitative insights into how the virus invades and spreads within the body through the eye. VISQUE Smart-LF was employed for ex vivo fluorescence imaging to observe enhanced green fluorescent protein (EGFP) expression, which verified the presence of SARS-CoV-2 in the eyes and brain of treated mice. The imaging demonstrated significant EGFP expression in the eyes and brain of both humanized ACE2 (hACE2) and ACE2 knockout (ACE2-KO) mice, confirming transocular infection.
期刊: J Med Virol. 2022 Jul 5;94(10):4878–4889.

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Insulin-incubated palladium clusters promote recovery after brain injury 2022

标题: Insulin-incubated palladium clusters promote recovery after brain injury
说明: The study aimed to synthesize insulin-incubated ultrasmall palladium clusters (Pd@insulin) and evaluate their therapeutic effects on traumatic brain injury (TBI) by scavenging reactive oxygen species (ROS). The VISQUE InVivo Smart-LF was used to trace the distribution of DR-labeled Pd@insulin clusters in mice. The imaging results indicated that Pd@insulin clusters were rapidly distributed throughout the body, with significant accumulation in the liver, kidney, spleen, and brain.
期刊: Biomacromolecules. 2022 Apr 22;23(8):3130–3141.

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High-Intensity Aerobic Exercise Suppresses Cancer Growth by Regulating Skeletal Muscle-Derived Oncogenes and Tumor Suppressors 2022

标题: High-Intensity Aerobic Exercise Suppresses Cancer Growth by Regulating Skeletal Muscle-Derived Oncogenes and Tumor Suppressors
说明: The study examines how high-intensity aerobic exercise suppresses cancer growth by regulating skeletal muscle-derived oncogenes and tumor suppressors. It explores the molecular mechanisms behind exercise's anticancer effects using a colorectal cancer mouse model. VISQUE InVivo Smart was used to monitor cancer cell metastasis in mice injected with colorectal cancer cells, enabling the tracking of cancer progression during the study. The in vivo imaging revealed significant tumor growth reduction in mice subjected to high-intensity aerobic exercise, confirming exercise's inhibitory effect on metastasis.
期刊: Front Mol Biosci. 2022 Jun 21;9:818470.

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Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway 2022

标题: Metformin Ameliorates Senescence of Adipose-Derived Mesenchymal Stem Cells and Attenuates Osteoarthritis Progression via the AMPK-Dependent Autophagy Pathway
说明: "The main goal of the paper is to investigate whether metformin can counteract the senescence of adipose-derived stem cells (ADSCs) and improve outcomes in osteoarthritis (OA) by reducing inflammation and cartilage degradation.
The Vieworks VISQUE in vivo imaging system was used to track the labeled ADSCs injected into the mice to assess their survival and localization in the knee joints at different time points (1, 3, and 7 days post-injection)(Oxidative Medicine and …). The imaging results showed that the fluorescence signal from the DiR-labeled ADSCs persisted up to 7 days, indicating that the cells survived and were active within the joint area during this period(Oxidative Medicine and …)."
期刊: Oxid Med Cell Longev. 2022 Jun 3;2022:4620254.

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Therapeutic strategies targeting uPAR potentiate anti–PD-1 efficacy in diffuse-type gastric cancer 2022

标题: Therapeutic strategies targeting uPAR potentiate anti–PD-1 efficacy in diffuse-type gastric cancer
说明: This study investigates the potential of targeting the urokinase-type plasminogen activator receptor (uPAR) as a therapeutic approach to enhance anti-PD-1 efficacy in treating diffuse-type gastric cancer (DGC). Smart-LF was utilized to perform bioluminescent imaging on mouse models, allowing researchers to monitor tumor growth and evaluate the effects of the uPAR-targeted therapy. The imaging demonstrated that the combination of anti-uPAR and anti-PD-1 therapies significantly suppressed tumor growth and improved survival rates in the treated mouse models.
期刊: Sci Adv. 2022 May 25;8(21):eabn3774.

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Quantitative Biodistribution and Pharmacokinetics Study of GMP-Grade Exosomes Labeled with 89Zr Radioisotope in Mice and Rats 2022

标题: Quantitative Biodistribution and Pharmacokinetics Study of GMP-Grade Exosomes Labeled with 89Zr Radioisotope in Mice and Rats
说明: "The study aimed to assess the quantitative biodistribution and pharmacokinetics of GMP-grade therapeutic exosomes labeled with zirconium-89 (89 Zr) after systemic intravenous administration in mice and rats.
The VISQUE InVivo Smart-LF imaging system was used for in vivo fluorescence imaging to track the distribution of DR-labeled exosomes in mice. The imaging results indicated that the exosomes were mainly distributed in the liver, with significant accumulation also observed in the kidney, lung, and spleen."
期刊: Pharmaceutics. 2022 May 24;14(6):1118.

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FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis 2022

标题: FGF-2 signaling in nasopharyngeal carcinoma modulates pericyte-macrophage crosstalk and metastasis
说明: The study investigates the impact of FGF-2 signaling on nasopharyngeal carcinoma (NPC) metastasis, focusing on its role in promoting pericyte-macrophage crosstalk to drive tumor spread. VISQUE Invivo Elite was utilized for imaging GFP+ metastatic nodules in a mouse model to assess tumor spread following FGF-2 manipulation. Imaging revealed increased metastatic nodules in FGF-2–overexpressing tumors, suggesting that FGF-2 amplifies tumor metastasis by facilitating pericyte-driven macrophage recruitment
期刊: JCI Insight. 2022 May 23;7(10):e157874.

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Fluorescence-Shadowing Nanoparticle Clusters for Real-Time Monitoring of Tumor Progression 2022

标题: Fluorescence-Shadowing Nanoparticle Clusters for Real-Time Monitoring of Tumor Progression
说明: "The study aims to develop a real-time tumor monitoring system using MMP-responsive gold nanoparticle (AuNP) clusters and quantum dots (QD) to detect tumor microenvironments, facilitating better therapeutic strategies.
The VISQUE InVivo Smart-LF system was utilized to visualize fluorescence changes in vivo, confirming the FRET-based quenching of QDs when the AuNPs and QDs formed clusters in response to MMP activity at tumor sites. The imaging results demonstrated significant fluorescence attenuation at tumor sites, which correlated with tumor size and enzyme levels, validating the system’s potential for tumor progression monitoring"
期刊: Biomacromolecules. 2022 Apr 22;23(8):3130–3141.

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Immunotherapeutic effects of recombinant Bacillus Calmette–Guérin containing sic gene in ex vivo and in vivo bladder cancer models 2022

标题: Immunotherapeutic effects of recombinant Bacillus Calmette–Guérin containing sic gene in ex vivo and in vivo bladder cancer models
说明: The study aims to assess the efficacy of a recombinant Bacillus Calmette-Guérin (rBCG-sic) with enhanced immunotherapeutic effects on bladder cancer, targeting improved response rates in non-muscle invasive bladder cancer. VISQUE In Vivo Smart-LF was utilized to perform bioluminescence imaging to monitor bladder cancer progression in an orthotopic mouse model after rBCG-sic treatment. Imaging results showed that rBCG-sic significantly reduced tumor volume and slowed cancer progression compared to standard BCG, supporting its potential for greater anticancer efficacy.
期刊: Investig Clin Urol. 2022 Feb 23;63(2):228–237.

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A micellized bone morphogenetic protein-7 prodrug ameliorates liver fibrosis by suppressing transforming growth factor-β signaling 2022

标题: A micellized bone morphogenetic protein-7 prodrug ameliorates liver fibrosis by suppressing transforming growth factor-β signaling
说明: "The main goal of the paper is to investigate the ability of a micellized bone morphogenetic protein-7 (BMP-7) prodrug to ameliorate liver fibrosis by suppressing transforming growth factor-β (TGF-β) signaling.
The Vieworks VISQUE in vivo Smart imaging system was used to acquire fluorescence images of mice administered with indocyanine green-labeled mPTD-BMP-7 to analyze its distribution in the liver. The imaging results showed that mPTD-BMP-7 was mainly delivered to the liver, indicating efficient hepatic delivery."
期刊: Am J Cancer Res. 2022 Feb 15;12(2):763–778.

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A novel dual-labeled small peptide as a multimodal imaging agent for targeting wild-type EGFR in tumors 2022

标题: A novel dual-labeled small peptide as a multimodal imaging agent for targeting wild-type EGFR in tumors
说明: The study aims to develop and evaluate a dual-labeled imaging agent, Tc-99m SYPIPDT-ECG-TAMRA, for targeted imaging of tumors that overexpress wild-type epidermal growth factor receptor (wtEGFR). VISQUE In Vivo Smart-LF was employed for ex vivo fluorescence imaging to assess TAMRA-labeled agent uptake in excised tumor and organ tissues from mice. Imaging confirmed higher uptake of the agent in wtEGFR-positive tumors compared to wtEGFR-negative ones, demonstrating the agent's specificity for wtEGFR-positive cells.
期刊: PLoS One. 2022 Feb 4;17(2):e0263474

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A salicylaldehyde benzoyl hydrazone based near-infrared probe for copper(ii) and its bioimaging applications 2022

标题: A salicylaldehyde benzoyl hydrazone based near-infrared probe for copper(ii) and its bioimaging applications
说明: This study develops a near-infrared (NIR) fluorescent probe, CySBH, for selective detection and imaging of copper (Cu²⁺) ions, aiming to aid in diagnosing copper-related diseases. VISQUE In Vivo Elite was employed for in vivo imaging to track the probe’s fluorescence response to Cu²⁺ in mice, visualizing its distribution and quenching in real-time. Imaging showed that the probe exhibited strong NIR fluorescence, which decreased with increased Cu²⁺ concentration, confirming the probe’s effective and selective response to Cu²⁺ ions in living subjects.
期刊: RSC Adv. 2022 Jan 24;12(5):3073–3080.

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Korean Red Ginseng Enhances Immunotherapeutic Effects of NK Cells via Eosinophils in Metastatic Liver Cancer Model 2022

标题: Korean Red Ginseng Enhances Immunotherapeutic Effects of NK Cells via Eosinophils in Metastatic Liver Cancer Model
说明: This study explores how Korean Red Ginseng (KRG) combined with natural killer (NK) cell therapy enhances the immune response to suppress metastasis in a metastatic liver cancer model. VISQUE In Vivo Elite was used for bioluminescent imaging to track metastasis progression in vivo within the metastatic liver cancer model. The imaging revealed that the combination of KRG and NK cells significantly inhibited metastatic progression, reducing bioluminescence intensity compared to other treatment groups, thereby indicating an enhanced anti-cancer effect.
期刊: Nutrients. 2021 Dec 28;14(1):134.

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Real-Time Longitudinal Evaluation of Tumor Blood Vessels Using a Compact Preclinical Fluorescence Imaging System 2021

标题: Real-Time Longitudinal Evaluation of Tumor Blood Vessels Using a Compact Preclinical Fluorescence Imaging System
说明: This study aims to use a compact preclinical fluorescence imaging system to monitor tumor blood vessel dynamics over time, focusing on evaluating the effects of angiogenesis inhibitors in real-time. VISQUE In Vivo Smart-LF was used for time-lapse imaging of tumor vasculature in mice injected with indocyanine green (ICG), allowing for detailed visualization of blood flow and vessel volume changes as the tumor progressed. The imaging revealed that tumor blood flow and vessel volume increased with tumor growth but decreased following angiogenesis inhibitor treatment, demonstrating the system’s utility for monitoring vascular responses to therapy.
期刊: Biosensors (Basel). 2021 Nov 23;11(12):471

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Local drug delivery using poly(lactic-co-glycolic acid) nanoparticles in thermosensitive gels for inner ear disease treatment 2021

标题: Local drug delivery using poly(lactic-co-glycolic acid) nanoparticles in thermosensitive gels for inner ear disease treatment
说明: This study aims to develop a localized drug delivery system using poly(lactic-co-glycolic acid) (PLGA) nanoparticles in a thermosensitive gel for treating inner ear diseases, ensuring sustained release of dexamethasone (DEX) directly to the inner ear. VISQUE In Vivo Smart was utilized for in vivo fluorescence imaging to observe the biodistribution and retention of the coumarin-6-loaded PLGA nanoparticles in the inner ear after administration. Imaging revealed that the PLGA nanoparticle-gel formulation remained longer in the middle ear, allowing for a sustained release to the inner ear, as evidenced by prolonged fluorescence intensity up to 48 hours post-administration.
期刊: Drug Deliv. 2021 Oct 20;28(1):2268–2277

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BEX1 and BEX4 Induce GBM Progression through Regulation of Actin Polymerization and Activation of YAP/TAZ Signaling 2021

标题: BEX1 and BEX4 Induce GBM Progression through Regulation of Actin Polymerization and Activation of YAP/TAZ Signaling
说明: This study investigates how BEX1 and BEX4 proteins influence glioblastoma (GBM) progression by promoting actin polymerization and activating YAP/TAZ signaling, especially following radiotherapy. VISQUE In Vivo Smart-LF was utilized for bioluminescent imaging to monitor tumor growth and progression in GBM-bearing mouse models following treatments. Imaging demonstrated significant tumor growth inhibition when radiotherapy was combined with Latrunculin B, an actin polymerization inhibitor, indicating potential suppression of GBM aggressiveness.
期刊: Int J Mol Sci. 2021 Sep 11;22(18):9845

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Downregulated CLIP3 induces radioresistance by enhancing stemness and glycolytic flux in glioblastoma 2021

标题: Downregulated CLIP3 induces radioresistance by enhancing stemness and glycolytic flux in glioblastoma
说明: The paper aims to explore how downregulation of the protein CLIP3 contributes to radioresistance in glioblastoma by increasing stemness and glycolytic activity, and it investigates the potential of glimepiride, a diabetes drug, as a radiosensitizer to counteract this effect. Smart-LF was used to monitor tumor growth in mice models with orthotopic glioblastoma xenografts after treatment with radiation and glimepiride. The imaging revealed significant inhibition of tumor growth in glioblastoma models treated with a combination of glimepiride and radiation, suggesting an enhanced therapeutic effect compared to radiation alone.
期刊: J Exp Clin Cancer Res. 2021 Sep 6;40:282.

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Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen 2021

标题: Development of combination adjuvant for efficient T cell and antibody response induction against protein antigen
说明: The study aims to develop an adjuvant formulation combining DOTAP, CpG D35, and aluminum salt to enhance both T cell and antibody responses to protein antigens, thereby improving vaccine efficacy for infections and cancer. VISQUE in vivo Smart-LF was used to track antigen distribution in vivo, particularly focusing on how the adjuvant formulation influences antigen retention at the injection site and migration to lymph nodes. The system showed that the DOTAP-containing formulation retained antigens at the injection site and increased antigen presence in draining lymph nodes over time, suggesting improved delivery and immune response initiation.
期刊: PLoS One. 2021 Aug 2;16(8):e0254628

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CKAP4 Antibody-Conjugated Si Quantum Dot Micelles for Targeted Imaging of Lung Cancer 2021

标题: CKAP4 Antibody-Conjugated Si Quantum Dot Micelles for Targeted Imaging of Lung Cancer
说明: The paper investigates the use of CKAP4 antibody-conjugated silicon quantum dot micelles (Si QD micelles-CKAP4) as a targeted imaging agent for lung cancer, aiming to improve fluorescence-guided surgery. Smart-LF was utilized for in vivo fluorescence imaging to track the biodistribution and targeting efficacy of Si QD micelles-CKAP4 in tumor-bearing mice. The imaging results showed strong fluorescence in lung cancer tissues compared to healthy tissues, confirming the Si QD micelles-CKAP4’s potential for specific targeting in lung cancer imaging.
期刊: Nanoscale Res Lett. 2021 Jul 31;16:124.

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Enzyme-Loaded pH-Sensitive Photothermal Hydrogels for Mild-temperature-mediated Combinational Cancer Therapy 2021

标题: Enzyme-Loaded pH-Sensitive Photothermal Hydrogels for Mild-temperature-mediated Combinational Cancer Therapy
说明: The study aims to create a glucose oxidase-loaded, pH-sensitive photothermal hydrogel that can combine mild-temperature photothermal therapy (PTT) and starvation therapy to treat solid tumors effectively and safely. VISQUE in vivo Smart-LF was utilized to capture bioluminescence images, assessing the anti-metastasis effects of the hydrogels by measuring bioluminescence intensity in lung tissues. Imaging with the system revealed significantly reduced lung bioluminescence signals in mice treated with the hydrogel under laser irradiation, indicating effective inhibition of tumor metastasis to the lungs.
期刊: Front Chem. 2021 Jul 29;9:736468.

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Artificially engineered antiferromagnetic nanoprobes for ultra-sensitive histopathological level magnetic resonance imaging 2021

标题: Artificially engineered antiferromagnetic nanoprobes for ultra-sensitive histopathological level magnetic resonance imaging
说明: The study aims to develop ultra-sensitive antiferromagnetic nanoparticle probes for high-resolution, non-invasive magnetic resonance imaging (MRI) at the histopathological level, with applications for detecting microscopic tumors and micrometastases. VISQUE InVivo Elite was used to capture fluorescence images of liver tissues in mice post-MRI to validate tumor targeting and distribution of the nanoprobe. Imaging with the VISQUE system confirmed specific targeting and strong fluorescence signals within microscopic tumors, corroborating the MRI findings and illustrating the probes' effectiveness in detecting small-scale tumors.
期刊: Nat Commun. 2021 Jun 22;12:3840

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Marriage of Virus‐Mimic Surface Topology and Microbubble‐Assisted Ultrasound for Enhanced Intratumor Accumulation and Improved Cancer Theranostics 2021

标题: Marriage of Virus‐Mimic Surface Topology and Microbubble‐Assisted Ultrasound for Enhanced Intratumor Accumulation and Improved Cancer Theranostics
说明: The study aims to improve intratumor accumulation and therapeutic efficacy of nanoparticles for cancer treatment by combining virus-mimic surface topology with microbubble-assisted ultrasound. VISQUE in vivo Elite was used to conduct in vivo fluorescence imaging, selecting the ICG channel to assess nanoparticle accumulation in the tumor. The system successfully detected enhanced fluorescence intensity in tumors after treatment, demonstrating increased intratumor accumulation due to the combined nanoparticle design and ultrasound strategy.
期刊: Adv Sci (Weinh). 2021 May 14;8(13):2004670.

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In Vitro Photodynamic Effects of the Inclusion Nanocomplexes of Glucan and Chlorin e6 on Atherogenic Foam Cells 2021

标题: In Vitro Photodynamic Effects of the Inclusion Nanocomplexes of Glucan and Chlorin e6 on Atherogenic Foam Cells
说明: The study investigated the in vitro photodynamic effects of Glucan/Chlorin e6 (Glu/Ce6) nanocomplexes on atherogenic foam cells as a potential treatment for atherosclerosis. The researchers used VISQUE in vivo Smart-LF to capture fluorescence images of the Glu/Ce6 nanocomplexes and their singlet oxygen generation under laser irradiation. The imaging showed successful internalization of the Glu/Ce6 nanocomplexes in foam cells, confirming efficient delivery of the photosensitizer Ce6 to targeted cells and demonstrating high singlet oxygen generation upon laser exposure.
期刊: Int J Mol Sci. 2020 Dec 26;22(1):177

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Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer 2021

标题: Hyperactivation of HER2-SHCBP1-PLK1 axis promotes tumor cell mitosis and impairs trastuzumab sensitivity to gastric cancer
说明: 曲妥珠单抗是 HER2 靶向治疗胃癌的主要成分，但由于细胞敏感性和耐药性不足，导致对患者治疗效果不佳。在这篇文章中，研究发现HER2通过过度激活关键的HER2-SHCBP1-PLK1轴，参与促进肿瘤发生的细胞有丝分裂和患者对曲妥珠单抗治疗的反应不良的过程。茶黄素-3,3'-双没食子酸（Theaflavine-3, 3’-digallate）是SHCBP1-PLK1相互作用的抑制剂，它是一种潜在的曲妥珠单抗增敏剂，与曲妥珠单抗联合使用，在抑制HER2阳性胃癌生长方面非常有效。在对 NCI-N87/luc 肿瘤异种移植模型小鼠进行曲妥珠单抗敲低 SHCBP1 或 TFBG 治疗后，使用 Visque InVivo Smart-LF 进行体内生物发光成像，观察 NCI-N87 肿瘤对曲妥珠单抗的纵向反应。
期刊: Nature Communications 2021 May 14;12(1):2812

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Lung-selective 25-hydroxycholesterol nanotherapeutics as a suppressor of COVID-19-associated cytokine storm 2021

标题: Lung-selective 25-hydroxycholesterol nanotherapeutics as a suppressor of COVID-19-associated cytokine storm
说明: 25-羟基胆固醇（25-hydroxycholesterol，25-HC）的降低和细胞因子水平的升高被认为在COVID-19的发病机制中起着关键作用，会诱发急性呼吸窘迫综合征 (Acute Respiratory Distress Syndrome，ARDS) 和败血症。在这篇文章中，研究者研发了25-HC 和双十二烷基二甲基溴化铵 (DDAB) 纳米囊泡 (25-HC@DDAB) 作为 COVID-19的候选药物，用于重症SARS-CoV-2 患者恢复细胞内胆固醇水平和抑制细胞因子风暴。25-HC@DDAB能够选择性地积在肺组织积聚，并有效降低COVID-19患者源性PBMCs中的炎性细胞因子水平。研究人员通过尾静脉将25-HC@DDAB注射到小鼠体内，并在特定时间点使用Visque InVivo Smart对离体组织进行体外荧光成像，证实了25-HC@DDAB在肺部的积聚。
期刊: Nano Today 2021 Jun; 38: 101149 2021 Jan 11;

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RNA interference-mediated suppression of TNF-α converting enzyme as an alternative anti-TNF-α therapy for rheumatoid arthritis 2021

标题: RNA interference-mediated suppression of TNF-α converting enzyme as an alternative anti-TNF-α therapy for rheumatoid arthritis
说明: 对于大约90%的类风湿关节炎（Rheumatoid Arthritis，RA）患者来说，抗TNF-α治疗被作为一线免疫治疗，因为过量的肿瘤坏死因子-α（TNF-α）与RA的发病机制有关。在这篇文章中，研究者提出通过抑制TNF-α转换酶（TNF-α Converting Enzyme，TACE）来下调TNF-α水平。在本文中，研究人员提出通过抑制TNF-α转化酶（TACE）作为一种新的方法来下调TNF-α水平。他们通过抑制 TACE 的 shRNA (shTACE) 和骨吸收位点特异性肽载体之间形成的非病毒复合物，研发了一种疾病位点靶向 RNA 干扰系统。在胶原诱导的关节炎 (Collagen Induced Arthritis，CIA) 模型实验中，shTACE/肽载体复合物通过增强的抗炎和抗破骨细胞作用减轻了关节炎症状。并且，从 RA 患者的组织和滑液中进行的人源细胞实验也获得了类似的结果。将 shTACE/肽载体复合物通过静脉注射到 CIA 模型小鼠体内后，使用 Visque InVivo Smart 进行荧光成像，观察 shTACE/肽载体复合物在小鼠体内的生物分布。
期刊: Journal of Controlled Release 2021 Feb 10;330:1300-1312

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Fenton-like reaction, glutathione reduction, and photothermal ablation-built-in hydrogels crosslinked by cupric sulfate for loco-regional cancer therapy 2021

标题: Fenton-like reaction, glutathione reduction, and photothermal ablation-built-in hydrogels crosslinked by cupric sulfate for loco-regional cancer therapy
说明: 在这篇文章中，研究者使用交联水凝胶系统研发了 HC/Cu/ICG 水凝胶，以将类芬顿反应相关化学动力学疗法（chemodynamic therapy，CDT）和热疗诱导光热疗法（photothermal therapy，PTT) 应用于局部区域癌症治疗。HC/Cu/ICG 水凝胶是通过结合硫酸铜 (Cu)、儿茶酚官能化透明质酸 (HC) 和吲哚菁绿 (ICG) 制备的。在体内实验中，发现HC/Cu/ICG水凝胶在肿瘤局部区域CDT和PTT中均有治疗效果，同时没有诱发任何严重的全身毒性反应。将 HC/Cu/ICG 水凝胶皮下注射到小鼠体内的第0、1、2、7、14和21天，使用Visque InVivo Smart进行体内荧光成像，研究 HC/Cu/ICG 水凝胶的生物降解性。
期刊: Biomaterials Science 2021 Feb 7;9(3):847-860

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Dissecting the impact of target-binding kinetics of protein binders on tumor localization 2021

标题: Dissecting the impact of target-binding kinetics of protein binders on tumor localization
说明: 为了提高蛋白质疗法的治疗效果，对其体内行为进行了系统的考虑和控制。在该研究中，通过在癌症模型小鼠上使用四种对EGFR具有不同亲和力的多重复结构的类抗体支架蛋白（repebodies）来研究蛋白质的结合亲和力对肿瘤定位的影响。研究发现通过平衡靶标结合和分离的最佳亲和力，可以克服结合位点屏障效应，促进蛋白质结合物在肿瘤中的深度渗透和积累。将不同的多重复结构的类抗体支架蛋白静脉注射到A431肿瘤异种移植模型小鼠体内后，在特定时间点使用Visque InVivo Smart LF进行体内荧光成像，观察多重复结构的类抗体支架蛋白的体内生物分布同时进行量化分析。
期刊: iScience 2021 Feb 19; 24(2): 102104.

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Antibreast Cancer Activity of Aspirin-Conjugated Chalcone Polymeric Micelles 2021

标题: Antibreast Cancer Activity of Aspirin-Conjugated Chalcone Polymeric Micelles
说明: 在这篇文章中，研究者使用 mPEG-PLA 共聚物开发了新型负载阿司匹林偶联查耳酮衍生物的纳米颗粒（AS-DK143-loaded NP）。AS-DK143 NP在体外细胞实验中显示4T1细胞的细胞活力降低，并且在4T1-luc细胞荷瘤小鼠中，具有选择性靶向抗癌和抑制肿瘤生长的疗效。将AS-DK143 NP皮下注射到4T1异种移植小鼠体内后，使用Visque InVivo Smart对在特定时间点解剖的离体组织进行荧光成像，确定其在体内的生物分布。
期刊: Macromolecular Research 2021 Jan 28;29:105–110

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Meso-pyridinium substituted BODIPY dyes as mitochondria-targeted probes for the detection of cysteine in living cells and in vivo 2021

标题: Meso-pyridinium substituted BODIPY dyes as mitochondria-targeted probes for the detection of cysteine in living cells and in vivo
说明: 半胱氨酸（Cys）是一种生物硫醇，是一种抗氧化剂，可以对抗引起线粒体氧化损伤的ROS。研究人员开发了BDP-S-o-Py+作为荧光探针，取代了BODIPY的内消旋吡啶，BODIPY 是一种用于 Cys 特异性检测的线粒体靶向探针。BDP-S-o-Py+可检测体外活细胞线粒体Cys和体内内源性Cys。通过皮下注射将BDP-S-o-Py+和Cys注射到小鼠体内，使用Visque InVivo Smart进行荧光成像，BDP-S-o-Py+显示出令人满意的体内Cys成像能力。
期刊: Dyes and Pigments 2021 Mar;187:109089

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Characterization of adipose-derived stromal/stem cell spheroids versus single-cell suspension in cell survival and arrest of osteoarthritis progression 2021

标题: Characterization of adipose-derived stromal/stem cell spheroids versus single-cell suspension in cell survival and arrest of osteoarthritis progression
说明: 在本文中，研究人员在大鼠模型中测试了球形脂肪源性的基质/干细胞（Adipose-derived Stromal/stem Cells，ASCs）用于治疗手术诱发的骨关节炎（Osteoarthritis，OA）。与单细胞悬浮液中的ASC相比，在3D中培养的ASC球体具有更好的体内外细胞存活率和软骨形成潜力，从而可以更好的再生和阻止手术诱导的OA的发生。在关节内注射 ASCs 后的特定时间点，通过 Visque InVivo Smart 荧光体内成像跟踪手术诱导的 OA 大鼠膝关节中的 ASCs。
期刊: Journal of Biomedical Materials Research Part A 2021 Jun;109(6):869-878

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Optically activatable photosynthetic bacteria-based highly tumor specific immunotheranostics 2020

标题: Optically activatable photosynthetic bacteria-based highly tumor specific immunotheranostics
说明: 在本文中，研究了非致病性天然紫色光合细菌（Purple Photosynthetic Bacteria，PPSB）作为肿瘤细菌疗法中细菌的特性。PPSB 表现出强烈的 NIR-I 到 NIR-II 荧光特性、强大的光热转换能力、出色的活性氧生成能力和对比鲜明的光声效应，具有高度靶向的肿瘤消除和精确标记肿瘤位置的能力。短期禁食可显著提高细菌治疗的光学效果。将PPSB静脉注射到Colon26荷瘤小鼠体内后，在特定时间点，使用Visque InVivo Smart LF分别进行活体、离体器官荧光成像，通过PPSB的生物分布研究PPSB的肿瘤靶向性。
期刊: Nano Today 2020 Apr;37:101100

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Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta–Mediated Epithelial–Mesenchymal Transition 2020

标题: Micellized Protein Transduction Domain-Bone Morphogenetic Protein-7 Efficiently Blocks Renal Fibrosis Via Inhibition of Transforming Growth Factor-Beta–Mediated Epithelial–Mesenchymal Transition
说明: 胶束中融合 BMP-7 的蛋白质转导结构域 (Protein Transduction Domain，PTD) (mPTD-BMP-7) 是近年来发展起来一种治疗肾纤维化的有效疗法。mPTD-BMP-7 在体外和体内单侧输尿管梗阻模型中成功抑制了 TGF-β 介导的上皮间质转化过程。研究人员通过在猪的肾动脉中开发和应用mPTD-BMP-7动脉内给药，确定了mPTD-BMP-7的临床相关性。mPTD-BMP-7 通过肾动脉介入有效地输送到鲍氏囊并抑制猪单侧输尿管阻塞引起的肾纤维化。mPTD-BMP-7 通过肾动脉进行动脉内给药，使用 Visque InVivo Smart 在给药后的特定时间点对离体肾脏进行荧光成像，观察 mPTD-BMP-7 在肾脏中的生物分布。
期刊: Frontiers in Pharmacology 2020 Nov 19;11:591275

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Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer 2020

标题: Prodrug-Loaded Zirconium Carbide Nanosheets as a Novel Biophotonic Nanoplatform for Effective Treatment of Cancer
说明: 在这篇文章中，研究者将碳化锆（Zirconium Carbide，ZrC）纳米片作为深度PTT光敏剂与按需求设计的抗癌前药SN38 Nif相结合，开发了一种生物光子学纳米平台。合成的ZrC@prodrug生物光子纳米平台在体外和体内实验中显示出强大的抗癌作用。在小鼠模型中，ZrC@prodrug 系统显著抑制肿瘤的复发、转移、炎症和血管生成。在小鼠模型治疗后的特定时间点，使用 Visque InVivo Elite 对离体的肝脏肿瘤进行荧光成像，证实了 ZrC@prodrug 对肿瘤生长的协同抑制作用。
期刊: Advanced Science 2020 Nov 5;7(24):2001191

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Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis 2020

标题: Newly designed Protein Transduction Domain (PTD)-mediated BMP-7 is a potential therapeutic for peritoneal fibrosis
说明: 骨形态发生蛋白-7（BMP-7）是一种治疗性生长因子，可逆转许多纤维化疾病，包括腹膜透析（Peritoneal Dialysis，PD）引起的腹膜纤维化。研究人员开发了一种利用蛋白质转导结构域（Protein Transduction Domains，PTD）的新型药物递送系统，并将其应用于BMP-7上制备了PTD-BMP-7。在大鼠的体内研究中，PTD-BMP-7 通过恢复腹膜的变化，并抑制已建立的 PD 纤维化的发展，显示出对上皮间充质转化 (Epithelial-Mesenchymal Transition，EMT) 相关纤维化的治疗作用。研究者在小鼠腹腔注射PTD-BMP-7后的特定时间点，通过Visque InVivo Elite进行荧光活体成像，通过Visque InVivo Smart进行荧光体外成像，研究了PTD-BMP-7的生物分布和半衰期。
期刊: Journal of Cellular and Molecular Medicine 2020 Nov;24(22):13507-13522

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“Star” miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer 2020

标题: “Star” miR-34a and CXCR4 antagonist based nanoplex for binary cooperative migration treatment against metastatic breast cancer
说明: 这篇文章通过糊精修饰1.8 k PEI与CM末端（Dextrin-PEI- cm, DPC）和细胞粘附分子CD44抑制剂Star miR-34a的自组装制备了DPC/miR-34a纳米复合物。DPC/miR-34a纳米复合物在体外细胞侵袭实验和体内抗转移模型中显示出良好的抑制癌细胞侵袭的能力。并且，DPC/miR-34a 在肺转移模型和原位 MDA-MB-231 肿瘤模型中均表现出优异的抗肿瘤和抗转移功效。通过小鼠尾静脉将 DPC/miR-34a 注射到 MDA-MB-231 肿瘤模型小鼠体内，在特定时间点通过 Visque InVivo Elite 的荧光体内和体外成像观察DPC/miR-34a 的生物分布。
期刊: Journal of Controlled Release 2020 Oct 10;326:615-627

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Ferritin Nanocage-Based Methyltransferase SETD6 for COVID-19 Therapy 2020

标题: Ferritin Nanocage-Based Methyltransferase SETD6 for COVID-19 Therapy
说明: 在这篇文章中，基于铁蛋白纳米笼的肽递送系统用于递送细胞穿透肽（cell-penetrating peptide）和带有 SETD6 的核定位 TAT-NBD 肽。TAT NBD-short ferritin-SETD6 (TFS) 增强 SETD6 水平并降低nuclear factor-κB (NF-κB) 信号。在小鼠体内静脉注射TFS后的特定时间点，使用Visque InVivo Smart对离体器官进行荧光成像，观察TFS在细胞因子风暴模型小鼠体内的生物分布。
期刊: Advanced Functional Materials 2020 Nov 25;30(48):2006110

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A brain tumor-homing tetra-peptide delivers a nano-therapeutic for more effective treatment of a mouse model of glioblastoma 2020

标题: A brain tumor-homing tetra-peptide delivers a nano-therapeutic for more effective treatment of a mouse model of glioblastoma
说明: 器官特异性细胞穿透肽（Organ-specific cell-penetrating peptides，CPPs）是一种非常有效地递送药物的分子，目前在癌症治疗中受到广泛关注。在本文中，研究人员开发了氨基酸序列丝氨酸-异亮氨酸-酪氨酸-缬氨酸（Serine-isoleucine-tyrosine-valine，SIWV）作为靶向多形性胶质母细胞瘤（Glioblastoma Multiforme，GBM）脑肿瘤组织的新CPPs。体外和体内实验表明，SIWV的体内靶向性增强。将与抗癌药物SN-38连接的SIWV注射到GBM异种移植小鼠体内后，在特定时间点使用Visque InVivo Smart LF进行体内和体外荧光成像，以确认其生物分布和作为靶向剂的能力。
期刊: Nanoscale Horizons 2020 Jul 27;5(8):1213-1225

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Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature 2020

标题: Jet-Lagged Nanoparticles Enhanced Immunotherapy Efficiency through Synergistic Reconstruction of Tumor Microenvironment and Normalized Tumor Vasculature
说明: 在该研究中，研究人员开发了两种时差纳米粒（ jet-lagged nanoparticles），一种是用于血管正常化治疗的apatinib (APA)-loaded TEC-targeting nanodrug（APA/MCP），另一种是用于肿瘤细胞代谢治疗的 lonidamine (LND)-loaded tumor cell-targeting nanodrug（LND/MCA）。APA/MCP可阻断VEGF/VEGFR2抑制肿瘤内皮细胞（ Tumor Endothelial Cells，TECs）增殖，LND/MCA可抑制乳酸（Lactic Acid，LA）外流以重塑肿瘤酸代谢。此外，体内药效学研究结果表明，带有时差纳米粒的程序性死亡1（PD-1）药物的治疗效果比PD-1组提高了3倍。将时差纳米粒注射到B16F 10荷瘤小鼠体内后，在特定时间点，使用Visque InVivo Elite进行荧光活体成像和离体器官成像，确定时差纳米粒的肿瘤靶向性和生物分布。
期刊: Advanced Healthcare Materials 2020 Jun;9(12):e2000075

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Dual functional nanoparticles containing SOX duo and ANGPT4 shRNA for osteoarthritis treatment 2020

标题: Dual functional nanoparticles containing SOX duo and ANGPT4 shRNA for osteoarthritis treatment
说明: 在该研究中，研究人员设计了包含地塞米松偶联聚乙烯亚胺（DEXPEI）与含有SOX duo（SOX-9，-6）和ANGPTL4小发夹RNA（shANG）[MCSOX9/6/shANG]的小环质粒（Minicircle plasmid，MC）复合物的纳米颗粒，以增强干细胞的软骨生成并抑制OA的炎症。转染 MCSOX9/6/shANG (MCSOX9/6/shANG-tADSCs) 的脂肪源性干细胞 (ADSCs)，在体外和体内实验中均可增强软骨的形成并抑制骨关节炎 (OA) 的发展。将荧光标记的MCSOX9/6/shANG-tADSCs注射到手术诱发OA的大鼠右膝关节后，在特定时间点，使用Visque InVivo Elite进行活体荧光成像，对MCSOX9/6/shANG-tADSCs进行追踪定位。
期刊: Journal of Biomedical Materials Research Part B: Applied Biomaterials 2020 Jan;108(1):234-242

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Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery 2020

标题: Neutrophil-like Cell-Membrane-Coated Nanozyme Therapy for Ischemic Brain Damage and Long-Term Neurological Functional Recovery
说明: 在该文中，研究人员提出了一种中性粒细胞样细胞膜包裹的介孔普鲁士蓝纳米酶（MPBzyme @ NCM），作为缺血性中风的非侵入性主动靶向疗法。纳米酶具有强大的抗炎和抗氧化应激特性，但通过非侵入性给药在缺血脑中积累不足。该研究研发了一种MPBzyme @ NCM，通过改善纳米酶对受损大脑的药物递送，从而对缺血性中风进行无创主动靶向治疗。MPBzyme @ NCM在递送到受损大脑后显示出对缺血性中风的长期体内治疗功效。通过Visque InVivo Elite的体内荧光成像证实了MPBzyme @ NCM在受损大脑中的积累。
期刊: ACS Nano

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Interrogation of Folic Acid-Functionalized Nanomedicines: The Regulatory Roles of Plasma Proteins Reexamined 2020

标题: Interrogation of Folic Acid-Functionalized Nanomedicines: The Regulatory Roles of Plasma Proteins Reexamined
说明: 叶酸受体-α（FR-α）在许多人类癌症中均过度表达，因此叶酸（FA）已被广泛用于促进纳米药物向肿瘤的靶向递送。该研究发现FA官能化可在脂质体表面诱导大量天然IgM的吸收，阻止FA与受体结合并促进体内补体激活。此外，FA官能化的聚合物纳米颗粒也容易受到天然IgM吸收的影响。使用Visque InVivo Elite的体内荧光成像技术，在尾静脉注射后的特定时间点观察FA官能化脂质体（FA-sLip）的生物分布。
期刊: ACS Nano

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Supramolecular Container-Mediated Surface Engineering Approach for Regulating the Biological Targeting Effect of Nanoparticles 2020

标题: Supramolecular Container-Mediated Surface Engineering Approach for Regulating the Biological Targeting Effect of Nanoparticles
说明: 在该文中，研究人员提出了一种基于超分子容器的表面工程方法，来构建一个化学桥，将纳米粒子和靶向配体结合在一起。研究人员使用无环葫芦素（aCB）分子容器作为超分子容器，通过双向主客体络合将纳米颗粒和靶向配体结合，实现更好的水分散性和增强的生物靶向性。aCB修饰的纳米颗粒在体内外实验中显示出增强的生物靶向性和靶向成像性能。通过静脉注射异硫氰酸罗丹明B（RITC）标记的纳米颗粒，在注射后的特定时间点使用Visque InVivo Elite对荷瘤小鼠进行荧光成像，证明了增强的靶向性和成像性能。
期刊: Nano Letters

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Enhancement of Nanozyme Permeation by Endovascular Interventional Treatment to Prevent Vascular Restenosis via Macrophage Polarization Modulation 2020

标题: Enhancement of Nanozyme Permeation by Endovascular Interventional Treatment to Prevent Vascular Restenosis via Macrophage Polarization Modulation
说明: 在该文中，研究人员检验了纳米酶（Nanozymes）作为血管内介入治疗后血管再狭窄的有效治疗策略。ROS是加重血管再狭窄的主要因素，纳米酶可清除ROS，但不能有效地通过血管内皮细胞屏障到达血管内膜。研究发现通过血管内介入治疗内皮细胞脱落增强了纳米颗粒的血管渗透，并通过巨噬细胞的摄取减轻了体内长期的血管再狭窄。该研究使用Visque InVivo Elite，在普鲁士蓝纳米酶（PBzyme）的作用下，对小鼠左颈总动脉进行体内荧光成像，确定血管球囊损伤（VBI）后血管再狭窄得以缓解。
期刊: Advanced Functional Materials

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A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues 2020

标题: A molecular approach to rationally constructing specific fluorogenic substrates for the detection of acetylcholinesterase activity in live cells, mice brains and tissues
说明: 在该文中，研究人员将二甲基氨基甲酸胆碱（dimethylcarbamate choline）和自分解支架（例如， 4-hydroxybenzyl alcohol）相结合，开发了用于乙酰胆碱脂酶（AChE）检测的新特异性底物。由于丁酰胆碱酯酶（BChE）和羧酸酯酶（CE）的干扰，与神经系统疾病密切相关的乙酰胆碱酯酶（AChE）活性的成像非常困难。具有代表性的底物P10能有效地消除CE和BChE的干扰，并在体外实验中通过AChE活性成像显示出较高的特异性。该研究使用Visque InVivo Elite进行在体和离体的荧光成像实验，成功地对正常小鼠大脑中的AChE活性进行成像。
期刊: Chemical Science

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Molecular Design of Conjugated Small Molecule Nanoparticles for Synergistically Enhanced PTT/PDT 2020

标题: Molecular Design of Conjugated Small Molecule Nanoparticles for Synergistically Enhanced PTT/PDT
说明: 在该文中，IID-ThTPA纳米粒子（IID-ThTPA NPs）被开发为同时进行光热疗法（PTT）和光动力疗法（PDT）的新型光热疗剂。研究人员设计了一种基于供体-受体-供体（D–A–D）的共轭小分子（IID-ThTPA）型光热敏剂，其中异靛蓝（IID）作为选择性受体，三苯胺（TPA）作为供体。这种IID-ThTPA在体外和体内显示出优异的肿瘤协同清除能力，并具有较高的PTT / PDT性能。该研究使用Visque InVivo Elite，在静脉注射后的特定时间点对提取的组织进行离体荧光成像，确定了IID-ThTPA纳米粒子在体内的生物分布。
期刊: Nano-Micro Letters

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In Vivo Imaging of Senescent Vascular Cells in Atherosclerotic Mice Using a β-Galactosidase-Activatable Nanoprobe 2020

标题: In Vivo Imaging of Senescent Vascular Cells in Atherosclerotic Mice Using a β-Galactosidase-Activatable Nanoprobe
说明: 检测衰老相关β-半乳糖苷酶（SA-β-Gal）可作为动脉粥样硬化等衰老相关疾病的早期诊断和治疗方法。
研究人员开发了一种可激活β-半乳糖苷酶的纳米探针BOD-L-βGal NPs来检测衰老细胞中SA-β-Gal的过度表达。纳米探针BOD-L-βGal-NPs在动脉中有良好的蓄积，因此在尾静脉注射后荧光显像成功地显示了动脉粥样硬化小鼠衰老细胞和血管系统。
小鼠尾静脉注射BOD-L-βGal纳米粒后，用Visque-InVivo-Smart进行活体荧光显像，观察对照组与衰老组的差异。
Visque-InVivo-Smart体外荧光成像证实了BOD-L-βGal-NPs在动脉中的蓄积。
期刊: Analytical Chemistry

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Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy 2020

标题: Discovery of a Monoiodo Aza-BODIPY Near-Infrared Photosensitizer: in vitro and in vivo Evaluation for Photodynamic Therapy
说明: Monoiodo Aza-BODIPY是一种新型的用于光动力治疗(PDT)的近红外光敏剂(PS)，是肿瘤治疗方法的一个新兴平台，其抗肿瘤疗效在体内和体外都得到了验证。
BODIPYs具有理想光敏剂（ps）特性的许多优点，其中aza-BODIPYs具有较大的吸收系数和近红外吸收，可以适应治疗窗(650 - 900nm)。研究人员对aza-BODIPY进行了多种替代变化，发现aza-BODIPY的单碘衍生物具有各种优点，是一种理想的光敏剂（PS），具有良好的光稳定性、化学稳定性和光细胞毒性。Aza-BODIPY在体内外均有较好的抗肿瘤作用。
用Visque活体智能检测小鼠体内和体外荧光成像技术，对尾静脉注射后特定时间点单碘虫Aza-BODIPY的生物分布进行了鉴定。
期刊: Journal of Medicinal Chemistry
2020 Sep 10;63(17):9950-9964

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Detecting Cysteine in Bioimaging with a Near‐Infrared Probe Based on a Novel Fluorescence Quenching Mechanism 2020

标题: Detecting Cysteine in Bioimaging with a Near‐Infrared Probe Based on a Novel Fluorescence Quenching Mechanism
说明: 研制了一种高选择性、高灵敏度的近红外开启荧光探针（BDP-NIR），用于检测生物系统中的半胱氨酸（Cys）。
当采用近红外光谱（NIR-105）检测大位移时。BDP-NIR在0～500μM范围内具有较高的线性动态范围，可用于Cys的定量检测。研究人员用Visque InVivo Elite对小鼠进行荧光成像，以确定在体内感知内源性Cys的能力。”
期刊: ChemBioChem

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Heme Oxygenase 1-Targeted Hybrid Nanoparticle for Chemo- and Immuno-Combination Therapy in Acute Myelogenous Leukemia 2020

标题: Heme Oxygenase 1-Targeted Hybrid Nanoparticle for Chemo- and Immuno-Combination Therapy in Acute Myelogenous Leukemia
说明: 这种新的杂交纳米颗粒被开发成一种治疗急性髓性白血病的新药物，并在人类AML原位小鼠模型中验证了其潜力。
脂-聚合物杂化纳米颗粒(hNP)装有锡中卟啉(SnMP)，一种血红素加氧酶1的抑制剂
(HO1)是一种抗氧化和细胞保护酶，可诱导化疗耐AML，也是一种白血病细胞靶向传递的工程抗体。抑制ho1的T-hNP (T-hNP/SnMP)增强了人类白血病细胞的化学敏感性。T-hNP/SnMP增强柔红霉素的化疗效果，通过重编程骨髓骨髓细胞增强免疫应答。
在离体研究中，研究者通过Visque活体智能小鼠荧光成像，确定T-hNP/SnMP在静脉注射后特定时间点的器官分布，并确认T-hNP/SnMP在骨髓中的存在。
期刊: Advanced Science
2020 Jul; 7(13): 2000487

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Penta-fluorophenol: A Smiles rearrangement-inspired cysteine-selective fluorescent probe for imaging of human glioblastoma 2020

标题: Penta-fluorophenol: A Smiles rearrangement-inspired cysteine-selective fluorescent probe for imaging of human glioblastoma
说明: 以缺电子五氟苯酚（NPO-B）为传感荧光探针，测定胶质母细胞瘤（GBM）的生物标志物半胱氨酸（Cys）水平。该探针具有选择性高、灵敏度高、响应时间快等优点，具有显著的荧光响应。研究者发现，该探针在小鼠模型和人体组织样本基础上，可通过Visque-in vivo-Elite的体外荧光成像，对GBM进行相对简单的诊断和示踪。
期刊: Chemical Science
2020 May 11;11(22):5658-5668

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Selenium and dopamine-crosslinked hyaluronic acid hydrogel for chemophotothermal cancer therapy 2020

标题: Selenium and dopamine-crosslinked hyaluronic acid hydrogel for chemophotothermal cancer therapy
说明: 以透明质酸（HA）-多巴胺（HD）为基础的亚硒酸钠（Se）定向交联水凝胶，包括吲哚青绿（ICG），被开发用于局部癌症治疗。在近红外激光照射下，HD/Se/ICG凝胶具有注射性好、生物降解性慢、光热效率高的特点，在不引起严重全身毒性的情况下具有良好的抑瘤作用。小鼠瘤内注射后用Visque-in vivo-Smart在特定时间点获取的活体荧光成像，检测凝胶在体内的生物降解性。
期刊: Journal of Controlled Release
2020 Apr 15;S0168-3659(20)30237-6.

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Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy 2020

标题: Surface-Initiated Atom Transfer Polymerized Anionic Corona on Gold Nanoparticles for Anti-Cancer Therapy
说明: 金纳米粒子（AuNPs）与siRNA形成层状结构，用于抗癌治疗。体外抗肿瘤作用证实AuNP具有较高的细胞凋亡率，并呈siRNA剂量依赖性地抑制癌基因表达。体内研究表明，用c-Myc-siRNA结合AuNPs治疗的小鼠肿瘤明显缩小。研究者利用Visque-in vivo-Smart荧光活体成像技术，观察c-Myc-siRNA结合AuNPs在小鼠体内的生物分布。
期刊: Pharmaceutics
2020 Mar; 12(3): 261.

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Multifunctional Dendrimer-Entrapped Gold Nanoparticles for Labeling and Tracking T Cells Via Dual-Modal Computed Tomography and Fluorescence Imaging 2020

标题: Multifunctional Dendrimer-Entrapped Gold Nanoparticles for Labeling and Tracking T Cells Via Dual-Modal Computed Tomography and Fluorescence Imagingy
说明: 将聚氨基胺（PAMAM）树枝状大分子包埋的金纳米粒子（Au-DENPs）与Fluo-4偶联，研制成一种高效的T细胞纳米探针。研究人员通过皮下注射T细胞后用Visque-in vivo-Smart拍摄小鼠体内的荧光图像，确定了这种纳米探针对T细胞的标记效率。
期刊: Biomacromolecules
2020 Apr 13;21(4):1587-1595.

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Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design 2019

标题: Functional Fragments of AIMP1-Derived Peptide (AdP) and Optimized Hydrosol for Their Topical Deposition by Box-Behnken Design
说明: 氨基酰-tRNA合成酶复合物相互作用多功能蛋白1（AIMP1）衍生肽（AdP），由于其成纤维细胞激活（Fibroblast-activating，FA）和黑素细胞抑制（Melanocyte-inhibiting，MI）功能，已被开发为皮肤抗衰老的药妆成分。然而，由于AdP的低渗透特性，需要一个合适的局部给药方法。本文研究人员开发了一种基于水溶胶（ hydrosol）的AdP片段局部给药系统，该系统可作用于成纤维细胞和黑素细胞。优化的水溶胶在 STM 中的肽沉积比游离 FA-AdP 高 127 倍。使用 Visque InVivo Smart 近红外荧光成像技术对 Strat-M membrane(STM) 中荧光染料标记的 FA-AdP 进行定量分析。
期刊: Molecules 2019 May 22;24(10):1967

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Smart Microcapsules with Molecular Polarity- and Temperature-Dependent Permeability 2019

标题: Smart Microcapsules with Molecular Polarity- and Temperature-Dependent Permeability
说明: 具有分子选择性渗透的微胶囊作为微反应器、胶囊型传感器、药物和细胞载体以及人造细胞等方面具有广泛的应用前景。在本文中，研究人员使用毛细管微流控装置从两种单体和十二醇中制备了water-in-oil-in-water （W/O/W)）双乳液滴。油壳（oil shell）在高于癸醇熔点时具有选择性渗透性，而在低于癸醇熔点时其选择渗透性则急剧降低。将载有 ICG 的油壳（oil shell）皮下注射到小鼠大腿后，在特定时间点使用 Visque InVivo Smart 通过体内荧光成像检测油壳（oil shell）的释放。
期刊: Small (Nano Micro Small) 2019 May;15(21):e1900434

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Oral Delivery of Honokiol Microparticles for Nonrapid Eye Movement Sleep 2019

标题: Oral Delivery of Honokiol Microparticles for Nonrapid Eye Movement Sleep
说明: Honokiol (HNK) 是一种从厚朴中提取的小分子木质素，可促进非快速眼动 (Nonrapid Eye Movement，NREM) 睡眠。然而，使用HNK治疗失眠受到其极低的口服生物利用率的限制。在该文中，研究人员通过将和厚朴酚负载到聚丙交酯-乙交酯酸微粒 (HNK-MP) 中制备了和厚朴酚微粒。在体内实验中，HNK-MP 的口服生物利用率增强，导致 AUC0-12h 和最大血药浓度（Cmax）增加，最终增加 NREM 睡眠。研究人员在体内测试了HNK-MP的生物安全性，并确定其对胃肠道没有损伤。在给大鼠口服HNK-MP后，使用Visque InVivo Elite对胃肠道进行体外荧光成像，以确定其在胃肠道内的滞留情况。
期刊: Molecular Pharmaceutics 2019 Feb 4;16(2):737-743

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Biodegradable Mesoporous Silica Achieved via Carbon Nanodots-Incorporated Framework Swelling for Debris-Mediated Photothermal Synergistic Immunotherapy 2019

标题: Biodegradable Mesoporous Silica Achieved via Carbon Nanodots-Incorporated Framework Swelling for Debris-Mediated Photothermal Synergistic Immunotherapy
说明: 均匀包覆CDs的介孔二氧化硅纳米颗粒有序骨架(CD@MSN)可用于光热疗法（PTT）和癌症免疫治疗。获得的CD@MSN在体内外均表现出较强的光热效应和靶向性积累。CD@MSN介导的PTT还显示了对肿瘤转移的免疫抑制作用，通过天然杀伤细胞和巨噬细胞。研究者通过Visque-InVivo-Smart对小鼠体内注射后特定时间点的离体器官进行荧光成像，来观察CD@MSN的分布。
期刊: Nano Letters
2019 Dec 11;19(12):8409-8417.

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Osteogenesis and angiogenesis are simultaneously enhanced in BMP2-/VEGF-transfected adipose stem cells through activation of the YAP/TAZ signaling pathway. 2019

标题: Osteogenesis and angiogenesis are simultaneously enhanced in BMP2-/VEGF-transfected adipose stem cells through activation of the YAP/TAZ signaling pathway.
说明: 在临界大小的颅骨缺损和长骨节段缺损的免疫抑制大鼠中，BMP2- / VEGF-转染的脂肪干细胞的成骨和血管生成作用得到了同时增强。作者使用Visque InVivo Elite系统进行成像发现，植入的干细胞到第56天仍未迁移出植入部位。于是他们提出了设想，在不适合生存的无血管环境中，可以使用基因细胞疗法快速的诱导血管和成骨生成。
期刊: Biomaterials Science
2019 Nov 1;7(11):4588-4602.

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Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance 2019

标题: Targeted delivery of CRISPR interference system against Fabp4 to white adipocytes ameliorates obesity, inflammation, hepatic steatosis, and insulin resistance
说明: 本文使用Visque InVivo Smart系统及其分析软件 Clevue，对体外实验中，CRISPR靶向干扰白色脂肪细胞中Fabp4的性能，进行了成像和定量研究。作者建议，通过使用CRISPR干扰系统，靶向干扰白色脂肪细胞中的Fabp4，可以为消退和治疗肥胖症和肥胖症引起的代谢综合征，提供了一种简单安全的方法。
期刊: Genome Research
2019 Sep;29(9):1442-1452.

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Conjugation of prostate cancer-specific aptamers to polyethylene glycol-grafted polyethylenimine for enhanced gene delivery to prostate cancer cells 2019

标题: Conjugation of prostate cancer-specific aptamers to polyethylene glycol-grafted polyethylenimine for enhanced gene delivery to prostate cancer cells
说明: 本文使用Visque InVivo Smart成像系统及其分析软件Clevue，对基因传递载体的生物分布进行了体外成像和定量。作者认为，PEI-PEG-Wy5a可能对前列腺癌特异性基因的传递有影响。
期刊: Journal of Industrial and Engineering Chemistry
2019 May 25;73:182-191

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A nanoscale photothermal agent based on a metal-organic coordination polymer as a drug-loading framework for effective combination therapy 2019

标题: A nanoscale photothermal agent based on a metal-organic coordination polymer as a drug-loading framework for effective combination therapy
说明: 在光热剂生物分布实验中，采用了本文所述的 Visque InVivo Elite 及其观测软件 Clevue 进行定量成像。
期刊: Acta Biomaterialia
2019 Aug;94:435-446.

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pH-Responsible Fluorescent Carbon Nanoparticles for Tumor Selective Theranostics via pH-turn on/off Fluorescence and Photothermal Effect In Vivo and In Vitro 2018

标题: pH-Responsible Fluorescent Carbon Nanoparticles for Tumor Selective Theranostics via pH-turn on/off Fluorescence and Photothermal Effect In Vivo and In Vitro
说明: 新的纳米颗粒是通过包含光热染料（IR825）负载的碳化两性离子聚合物[FNP-I]研发的。该纳米颗粒可作为 pH 响应荧光探针，感应细胞内癌细胞和通过到达癌细胞环境后激活的近红外 (Near Infrared，NIR) 可控光热疗法 (Photothermal Therapy，PTT)。在体外和体内实验中，该纳米颗粒显示出良好的细胞吸收和生物相容性，有望作为成像引导的光热治疗纳米工具用于癌症治疗。在这项研究中，研究人员通过静脉注射纳米颗粒后，使用Visque InVivo Elite对整个小鼠进行活体成像，确定了其在特定时间点的体内生物分布情况，并通过对处死后的离体器官成像，证实了纳米颗粒在肿瘤组织中的积聚。
期刊: Nanoscale 2018 Feb 1;10(5):2512-2523

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Age-related changes in pial arterial structure and blood flow in mice 2016

标题: Age-related changes in pial arterial structure and blood flow in mice
说明: 利用 Vieworks 观测软件 Clevue 的实时荧光成像技术和特征图分析技术研究了小鼠脑血流量和脉管系统的年龄相关的变化。
期刊: Neurobiology of Aging.
2016 Jan;37:161-170

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Exendin-4 protects hindlimb ischemic injury by inducing angiogenesis 2015

标题: Exendin-4 protects hindlimb ischemic injury by inducing angiogenesis
说明: 利用 Vieworks 观测软件 Clevue 的实时荧光成像和特征图分析两项技术对 exendin-4 在小鼠后肢缺血模型中的血管生成作用进行了分析。
期刊: Biochemical and Biophysical Research Communications.
2015 Oct 2;465(4):758-63.

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Use of indocyanine green for optical analysis of cortical infarcts in photothrombotic ischemic brains 2015

标题: Use of indocyanine green for optical analysis of cortical infarcts in photothrombotic ischemic brains
说明: 利用实时成像技术以及庆熙大学和 Vieworks 合作研究开发的 FluAngio 和 DyAngio 算法对小鼠脑部光化学诱导缺血性损伤进行了分析和诊断。CleVue 可以实现本文所述的等效分析。
期刊: Journal of Neuroscience Methods.
2015 Jun 15;248:46-50

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Optical measurement of mouse strain differences in cerebral blood flow using indocyanine green 2015

标题: Optical measurement of mouse strain differences in cerebral blood flow using indocyanine green
说明: 利用庆熙大学和 Vieworks 合作研究开发的特征图分析技术对两种小鼠的脑血流量差异进行了分析和比较。CleVue 可以实现本文所述的等效分析。
期刊: Journal of Cerebral Blood Flow & Metabolism.
2015 Jun;35(6):912-6

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Noninvasive Optical Measurement of Cerebral Blood Flow in Mice Using Molecular Dynamics Analysis of Indocyanine Green 2012

标题: Noninvasive Optical Measurement of Cerebral Blood Flow in Mice Using Molecular Dynamics Analysis of Indocyanine Green
说明: 利用 ICG 实时成像技术和荧光动力学分析技术研究了小鼠的脑血流量。CleVue 可以实现本文所述的等效分析。
期刊: PLOS ONE.
2012;7(10):e48383.

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Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature 2011

标题: Dynamic fluorescence imaging for multiparametric measurement of tumor vasculature
说明: 利用 ICG 实时荧光成像技术和分析算法对肿瘤脉管系统进行了成像和分析。CleVue 可以实现本文所述的等效分析。
期刊: Journal of Biomedical Optics
2011 Apr;16(4):046008.

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Efficient differentiation of human pluripotent stem cells into functional CD34 progenitor cells by combined modulation of the MEK/ERK and BMP4 signaling pathways 2010

标题: Efficient differentiation of human pluripotent stem cells into functional CD34 progenitor cells by combined modulation of the MEK/ERK and BMP4 signaling pathways
说明: 通过分析缺血组织的血流研究了干细胞疗法对小鼠缺血组织的血管生成作用。
期刊: BLOOD.
2010 Dec16;116(25):5762-72

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Unsorted human adipose tissue-derived stem cells promote angiogenesis and myogenesis in murine ischemic hindlimb model 2010

标题: Unsorted human adipose tissue-derived stem cells promote angiogenesis and myogenesis in murine ischemic hindlimb model
说明: 通过分析组织的荧光图像确认了干细胞治疗后对小鼠缺血后肢模型中血管生成和肌细胞生成的促进作用。
期刊: Microvascular Research.
2010 Dec;80(3):310-6.

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Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics 2009

标题: Quantitative Analysis of Peripheral Tissue Perfusion Using Spatiotemporal Molecular Dynamics
说明: 通过小鼠缺血后肢模型中的实时荧光成像和分析，研究了灌注速率与梗死的关系。
期刊: PLOS One. 2009;4(1):e4275.

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